LOCUS       sequence-196595-        9019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a sgRNA and a Cas9 EQR variant that recognizes ""NGAG"" 
            PAM motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Lambrus BG, Moyer TC, Holland AJ
  TITLE     Applying the auxin-inducible degradation system for rapid protein 
            depletion in mammalian cells.
  JOURNAL   Methods Cell Biol. 2018;144:107-135. doi: 
            10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5.
  PUBMED    29804665
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Cell Biol."; date: "2018"; volume: "144:107-135. doi"; pages: " 
            10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5"
COMMENT     Sequence Label: px459 EQR
FEATURES             Location/Qualifiers
     source          1..9019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          8..235
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      247..256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             256..321
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             328..348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             373..2088
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             4474..4521
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4537..4590
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4591..5190
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4591..4610)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5087..5107
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5218..5235)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5224..5431
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5440..5580
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5440..5569
                     /label=AAV2 ITR
     rep_origin      5655..6110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5742..5761)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5952..5973
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6127..6146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6246..6268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6306..6324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6392..6496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6497..6565,6566..7357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6715..6734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7528..8116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8017..8036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8178..8426
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8178..8198
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8349..8368
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8445..8520
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8617..8902
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
       61 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      121 ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat ggttggttgg
      181 tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt ttcaggttgg
      241 accggtgcca ccatggacta taaggaccac gacggagact acaaggatca tgatattgat
      301 tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga
      361 gtcccagcag ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc
      421 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
      481 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
      541 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
      601 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
      661 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
      721 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
      781 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
      841 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
      901 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
      961 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     1021 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     1081 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     1141 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     1201 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     1261 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     1321 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     1381 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     1441 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     1501 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     1561 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     1621 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     1681 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     1741 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     1801 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     1861 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     1921 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     1981 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     2041 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     2101 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     2161 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     2221 gatatagtgt tgactcttac cctctttgaa gatcgggaaa tgattgagga aagactaaaa
     2281 acatacgctc acctgttcga cgataaggtt atgaaacagt taaagaggcg tcgctatacg
     2341 ggctggggac gattgtcgcg gaaacttatc aacgggataa gagacaagca aagtggtaaa
     2401 actattctcg attttctaaa gagcgacggc ttcgccaata ggaactttat gcagctgatc
     2461 catgatgact ctttaacctt caaagaggat atacaaaagg cacaggtttc cggacaaggg
     2521 gactcattgc acgaacatat tgcgaatctt gctggttcgc cagccatcaa aaagggcata
     2581 ctccagacag tcaaagtagt ggatgagcta gttaaggtca tgggacgtca caaaccggaa
     2641 aacattgtaa tcgagatggc acgcgaaaat caaacgactc agaaggggca aaaaaacagt
     2701 cgagagcgga tgaagagaat agaagagggt attaaagaac tgggcagcca gatcttaaag
     2761 gagcatcctg tggaaaatac ccaattgcag aacgagaaac tttacctcta ttacctacaa
     2821 aatggaaggg acatgtatgt tgatcaggaa ctggacataa accgtttatc tgattacgac
     2881 gtcgatcaca ttgtacccca atcctttttg aaggacgatt caatcgacaa taaagtgctt
     2941 acacgctcgg ataagaaccg agggaaaagt gacaatgttc caagcgagga agtcgtaaag
     3001 aaaatgaaga actattggcg gcagctccta aatgcgaaac tgataacgca aagaaagttc
     3061 gataacttaa ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc cggatttatt
     3121 aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat actagattcc
     3181 cgaatgaata cgaaatacga cgagaacgat aagctgattc gggaagtcaa agtaatcact
     3241 ttaaagtcaa aattggtgtc ggacttcaga aaggattttc aattctataa agttagggag
     3301 ataaataact accaccatgc gcacgacgct tatcttaatg ccgtcgtagg gaccgcactc
     3361 attaagaaat acccgaagct agaaagtgag tttgtgtatg gtgattacaa agtttatgac
     3421 gtccgtaaga tgatcgcgaa aagcgaacag gagataggca aggctacagc caaatacttc
     3481 ttttattcta acattatgaa tttctttaag acggaaatca ctctggcaaa cggagagata
     3541 cgcaaacgac ctttaattga aaccaatggg gagacaggtg aaatcgtatg ggataagggc
     3601 cgggacttcg cgacggtgag aaaagttttg tccatgcccc aagtcaacat agtaaagaaa
     3661 actgaggtgc agaccggagg gttttcaaag gaatcgattc ttccaaaaag gaatagtgat
     3721 aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt cgagagccct
     3781 acagttgcct attctgtcct agtagtggca aaagttgaga agggaaaatc caagaaactg
     3841 aagtcagtca aagaattatt ggggataacg attatggagc gctcgtcttt tgaaaagaac
     3901 cccatcgact tccttgaggc gaaaggttac aaggaagtaa aaaaggatct cataattaaa
     3961 ctaccaaagt atagtctgtt tgagttagaa aatggccgaa aacggatgtt ggctagcgcc
     4021 ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat
     4081 ttagcgtccc attacgagaa gttgaaaggt tcacctgaag ataacgaaca gaagcaactt
     4141 tttgttgagc agcacaaaca ttatctcgac gaaatcatag agcaaatttc ggaattcagt
     4201 aagagagtca tcctagctga tgccaatctg gacaaagtat taagcgcata caacaagcac
     4261 agggataaac ccatacgtga gcaggcggaa aatattatcc atttgtttac tcttaccaac
     4321 ctcggcgctc cagccgcatt caagtatttt gacacaacga tagatcgcaa acagtacaga
     4381 tctaccaagg aggtgctaga cgcgacactg attcaccaat ccatcacggg attatatgaa
     4441 actcggatag atttgtcaca gcttgggggt gacaaaaggc cggcggccac gaaaaaggcc
     4501 ggccaggcaa aaaagaaaaa ggaattcggc agtggagagg gcagaggaag tctgctaaca
     4561 tgcggtgacg tcgaggagaa tcctggccca atgaccgagt acaagcccac ggtgcgcctc
     4621 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4681 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     4741 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4801 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4861 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4921 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     4981 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5041 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5101 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5161 tggtgcatga cccgcaagcc cggtgcctga gaattctaac tagagctcgc tgatcagcct
     5221 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5281 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5341 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5401 attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt
     5461 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     5521 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     5581 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     5641 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5701 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     5761 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5821 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     5881 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5941 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     6001 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     6061 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     6121 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     6181 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6241 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     6301 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     6361 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6421 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6481 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6541 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6601 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6661 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6721 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6781 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6841 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6901 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6961 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7021 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7081 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7141 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7201 cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7261 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7321 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7381 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7441 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8161 gccttttgct cacatgtgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     8221 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     8281 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     8341 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     8401 atatcttgtg gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa
     8461 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     8521 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
     8581 gcgccaattc tgcagacaaa tggctctaga ggtacccgtt acataactta cggtaaatgg
     8641 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg
     8701 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     8761 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     8821 ctggcattgt gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     8881 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     8941 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     9001 gatgggggcg ggggggggg
//
