LOCUS       sequence-19660-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Wang W, Budhu A, Forgues M, Wang XW
  TITLE     Temporal and spatial control of nucleophosmin by the Ran-Crm1 
            complex in centrosome duplication.
  JOURNAL   Nat Cell Biol. 2005 Aug . 7(8):823-30.
  PUBMED    16041368
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2005 Aug . 7(8):823-30."
COMMENT     Sequence Label: GFP-NPM NESM-NLS1D
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cncgggncnc nggcanggac gagctgtcaa gtccggactc agatctcgag ctcaagcttc
       61 gaattctgca gtcgacggta ccgcgggccc gggatccgcc gccaccatgg actacaagga
      121 tgacgacgac aagcatatgg aagattcgat ggacatggac atgagccccc tgaggcccca
      181 gaactatctt ttcggttgtg aactaaaggc cgacaaagat tatcacttta aggtggataa
      241 tgatgaaaat gagcaccagt tatctttaag aacggtcagt ttaggggctg gtgcaaagga
      301 tgagttgcac attgttgaag cagaggcaat gaattacgaa ggcagtccaa ttaaagtaac
      361 actggcaact ttgaaaatgt ctgtacagcc aacggtttcc cttgggggct ttgaaataac
      421 accaccagtg gtcgcaaggg cgaagtgtgg ttcagggcca gtgcatatta gtggacagca
      481 cttagtagct gtggaggaag atgcagagtc agaagatgaa gaggaggagg atgtgaagct
      541 ttctgcccct ggaggtggta gcaaggttcc acagaaaaaa gtaaaacttg ctgctgatga
      601 agatgatgac gatgatgatg aagaggatga tgatgaagat gatgatgatg atgattttga
      661 tgatgaggaa gctgaagaaa aagcgccagt gaagaaatct atnnnatatt ccccaccaat
      721 gntnnnnata attnctccaa atggaaanac tcaancctct cnccccatat caaaggacag
      781 atcntcagaa cagaaaactc taacacaaga ctagtcntaa gactaaccaa tcacattaaa
      841 aggngtcttc nannc
//
