LOCUS       sequence-196608-        7096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of PBL27-YFPc-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7096)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM-35s-PBL27-YFPc-1
FEATURES             Location/Qualifiers
     source          1..7096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    921..945
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1039..1291)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1311..1862)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1904..2083)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2552..2896
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2840..2860
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2898..2914
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2899..2915
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2931..2955
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4498..4522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4533..4784
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4721..4742
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5076..5100
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5212..5921
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5994..6085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6086..6154,6155..6946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6304..6323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgg actctagcta gaggatcaca ggcagcaacg ctctgtcatc gttacaatca
      721 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
      781 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
      841 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
      901 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
      961 caacttaata acacattgcg gacgttttta atgatcgaat actaacgtct ctaccagata
     1021 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
     1081 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
     1141 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
     1201 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
     1261 agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctaggggtca tcagatttcg
     1321 gtgacgggca ggaccggacg gggcggcacc ggcaggctga agtccagctg ccagaaaccc
     1381 acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat
     1441 ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg
     1501 ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt ggagcccagt
     1561 cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca gtcgtaggcg
     1621 ttgcgtgcct tccagggacc cgcgtaggcg atgccggcga cctcgccgtc cacctcggcg
     1681 acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc ctgcggttcc
     1741 tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac gatggtgcag
     1801 accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg ttctgggctc
     1861 atggtagatc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg
     1921 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     1981 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     2041 tccagaaacc cgcggctcag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta
     2101 aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc
     2161 atgatcagat tgtcgtttcc cgccttcggt ttgggcgcgc ccggttcaga agaccagagg
     2221 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     2281 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat
     2341 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat
     2401 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     2461 caagtggatt gatgtgatac atggtggagc acgacactct cgtctactcc aagaatatca
     2521 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
     2581 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     2641 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     2701 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
     2761 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     2821 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     2881 ttcatttgga gaggacctcg aggtcgacgg tatcgataag cttcccatca acaagtttgt
     2941 acaaaaaagc aggcttgatg agtgggtgtt tgccttgttt tggatcttcg gctaaagacg
     3001 ctgcttctaa agattcggtg aagaaggaac tttcagctaa agacggctca gttactcagt
     3061 ctcaccatat cagcttagat aaatcaaagt ctcgacgagg tcctgaacag aagaaggagc
     3121 taactgctcc aaaagaaggg cctactgcgc atattgctgc acaaaccttt actttccgag
     3181 agttagctgc cgccactaaa aactttcgac cggaatgtct tcttggagaa ggaggtttcg
     3241 gacgtgttta caaaggtcgt ctagagacca caggacagat agtagctgtt aaacagcttg
     3301 atcgaaacgg tctacaagga aacagagagt ttcttgtaga ggttcttatg ctgagccttc
     3361 tgcatcatcc caatcttgtg aatttgattg gttattgtgc tgatggggac cagcgtcttc
     3421 ttgtgtatga gtatatgcca ctaggatcat tggaggatca tctacacgat cttccacctg
     3481 ataaagagcc tctagactgg agtactagaa tgacaatagc ggcaggagca gcaaagggac
     3541 tggagtatct gcatgataaa gcgaatccgc ctgtgatcta cagagacctg aaatcatcca
     3601 acattcttct cggtgatggc tatcacccaa agttatctga ttttgggtta gctaagttag
     3661 gtcccgtggg cgataaaaca catgtgtcaa ctcgtgtgat gggcacatat ggttattgtg
     3721 caccggaata tgccatgaca gggcaactca cattgaaatc cgatgtttat agctttgggg
     3781 ttgtgtttct cgagctcatc acgggtcgaa aagctattga taatgctcga gcacccggag
     3841 agcacaacct tgtcgcatgg gctaggccgt tgttcaaaga tcgtagaaag tttccgaaga
     3901 tggcggatcc atcgctgcaa gggcggtatc caatgcgtgg tctatatcaa gcacttgcag
     3961 ttgcagcaat gtgtttacag gaacaagcag cgacaagacc actgattggc gacgtggtga
     4021 cagctctaac atacttagct tcgcaaacgt ttgacccaaa cgcaccaagc ggtcaaaaca
     4081 gtagaagtgg gagtgggcca ccatttatca gaacaaggga tgatcggagg agcttgggag
     4141 atgggagtag cttggatagt cctgcagaga ctcggagtcg gttagggtca ccagccactc
     4201 acaagaactc tcctgattac agaagaaggg atatggtgag ggaagtcaat gcaggatcag
     4261 aaggtgggag cgagacagga ggcgggtcag gtagaaaatg gggattaagc gatttggaag
     4321 ggcaagaatc acagagaggg agcccggcga gtgttgggag atcatcgaga ggcactccga
     4381 ggaaccgaga tttagataga gagagggcgg tggcggaagc aaaggtgtgg ggagagaatt
     4441 ggagggagag gaagagagct accaatggac cgggcagctt tgatagtaca aatgactacc
     4501 cagctttctt gtacaaagtg gttgatggga tggacaagca gaagaacggc atcaaggcca
     4561 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     4621 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     4681 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4741 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatct agagtccgca
     4801 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
     4861 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
     4921 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     4981 ttcctaaaac caaaatccag tgaccgggcg gccgccaccg cggtggaggg ggatcagatt
     5041 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     5101 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     5161 tccgttcgtc catttgtatg tgtacatcac cgacgagcaa ggcaagaccg agcgcctttc
     5221 cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa
     5281 acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg gccgccggcg ttgtggatac
     5341 ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc
     5401 gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg
     5461 agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg
     5521 atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact
     5581 gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg
     5641 cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc
     5701 cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata
     5761 aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt
     5821 gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct
     5881 gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcag tccttgccat
     5941 tgccgggatc ggggcagtaa cgggatgggc gatcagcccg aaagctaccc ctatttgttt
     6001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7081 agcgtcagac cccgta
//
