LOCUS       sequence-196612-        7435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of Slac1-YFPn-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM-35s-Slac1-YFPn-1
FEATURES             Location/Qualifiers
     source          1..7435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      30..618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     519..538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    930..954
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1048..1300)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1320..1871)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1913..2092)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2561..2905
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2849..2869
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     2907..2923
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2908..2924
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2940..2964
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4636..4660)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4668..4670,4671..4673,4674..5132)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(4713..4734)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4974..4993)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5424..5448
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5560..6269
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6342..6433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6434..6502,6503..7294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6652..6671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgga ctctagctag aggatcacag gcagcaacgc tctgtcatcg
      721 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
      781 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
      841 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
      901 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
      961 acgcttagac aacttaataa cacattgcgg acgtttttaa tgatcgaata ctaacgtctc
     1021 taccagatat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
     1081 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
     1141 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
     1201 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
     1261 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat
     1321 cagatttcgg tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc
     1381 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
     1441 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
     1501 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
     1561 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag
     1621 tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc
     1681 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
     1741 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
     1801 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
     1861 tctgggctca tggtagatcc cctcgatcga gttgagagtg aatatgagac tctaattgga
     1921 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
     1981 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     2041 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
     2101 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     2161 tctccgctca tgatcagatt gtcgtttccc gccttcggtt tgggcgcgcc cggttcagaa
     2221 gaccagaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     2281 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaaga tggcttctac
     2341 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt
     2401 cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg
     2461 tcttcaaagc aagtggattg atgtgataca tggtggagca cgacactctc gtctactcca
     2521 agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt caacaaaggg
     2581 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     2641 cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga aaggctatcg
     2701 ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg
     2761 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     2821 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     2881 gaagttcatt tcatttggag aggacctcga ggtcgacggt atcgataagc ttcccatcaa
     2941 caagtttgta caaaaaagca ggcttgatgg agaggaaaca gtcaaatgct cattccactt
     3001 tcgccgacat caatgaagtt gaagatgaag cagagcaaga actgcaacag caagagaaca
     3061 acaacaacaa gagattctct ggaaacagag gaccaaaccg agggaaacaa agaccattca
     3121 gaggttttag cagacaggtt tcgctagaga caggtttctc agtgctcaac agagaatcta
     3181 gagaaagaga tgacaaaaag agtttaccga ggagtggtcg tagctttggc gggtttgaat
     3241 caggtggaat catcaacggt ggagatggtc ggaaaacaga ttttagtatg tttaggacta
     3301 aatcaacgct cagcaaacaa aagtctttat tgccttctat aataagggag agagacattg
     3361 agaattcttt aagaactgaa gatggtgaga ccaaagatga ttctataaat gaaaatgttt
     3421 ctgcaggaag atactttgct gctcttagag gacctgaatt ggatgaagtc aaggacaatg
     3481 aagatattct gcttccaaaa gaggagcaat ggccgtttct cctaaggttc ccaattggat
     3541 gcttcggtat ctgtttggga cttagcagtc aagctgtctt gtggcttgcc ctagcgaaaa
     3601 gtcctgcaac aaacttttta cacatcacac cattgattaa cttagttgta tggctatttt
     3661 cactggtagt tttagtctct gtgtccttca cttacatact caaatgcatc ttctactttg
     3721 aagctgtcaa aagagagtat tttcatcctg ttcgagtcaa cttcttcttc gctccttggg
     3781 tggtttgcat gttcctagcc attagcgtac ctcccatgtt ctcaccaaac cgaaaatacc
     3841 tccatcccgc catttggtgt gttttcatgg gtccttattt cttcctcgag ctaaagatct
     3901 acgggcaatg gctttcggga gggaagaggc gactatgcaa agtcgcaaac ccatcttctc
     3961 atctttctgt tgtagggaat ttcgtcgggg ccatcttagc ttcaaaagtt ggatgggatg
     4021 aagtagctaa gtttctatgg gcagtaggtt ttgcacatta cttggttgtg tttgtaacac
     4081 tttatcagag acttcccacc agtgaagctc ttcctaaaga gcttcatcct gtttactcta
     4141 tgttcattgc tgccccatca gcagcaagta ttgcttggaa cacgatatat ggccaattcg
     4201 acggatgttc aagaacttgc ttcttcattg cactcttcct atacatatcc ctagttgctc
     4261 ggatcaattt cttcactgga tttaagttct cagtggcgtg gtggtcatat acttttccta
     4321 tgacaacagc atccgtagca acaataaagt atgcagaggc agtacctggt tacccgagcc
     4381 gggctctagc actcactctt tccttcattt ccacagcgat ggtctgcgtt ttgtttgtct
     4441 ccacgcttct ccacgccttt gtctggcaaa cgttgttccc aaacgatctt gcaattgcca
     4501 ttacaaagag gaaacttacc agggagaaga agccttttaa aagagcttat gacttgaagc
     4561 gctggaccaa gcaggctctg gcgaagaaaa tatctgcaga aaaagatttt gaagcagagg
     4621 aagagtcgca tcactaccca gctttcttgt acaaagtggt tgatgggatg gtgagcaagg
     4681 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4741 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4801 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4861 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     4921 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4981 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5041 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5101 actacaacag ccacaacgtc tatatcatgg cctaatctag agtccgcaaa aatcaccagt
     5161 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca
     5221 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     5281 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     5341 aaatccagtg accgggcggc cgccaccgcg gtggaggggg atcagattgt cgtttcccgc
     5401 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     5461 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     5521 tttgtatgtg tacatcaccg acgagcaagg caagaccgag cgcctttccg acgctcaccg
     5581 ggctggttgc cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa
     5641 cgccgtcgaa gccgtgtgcg agacaccggc cgccggcgtt gtggatacct cgcggaaaac
     5701 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     5761 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     5821 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     5881 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     5941 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     6001 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     6061 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     6121 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     6181 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
     6241 cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc cttgccattg ccgggatcgg
     6301 ggcagtaacg ggatgggcga tcagcccgaa agctacccct atttgtttat ttttctaaat
     6361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtca
//
