LOCUS       sequence-196628-       10934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of PDGFRa and CopGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M
  TITLE     Tmeff2 is expressed in differentiating oligodendrocytes but 
            dispensable for their differentiation in vivo.
  JOURNAL   Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1.
  PUBMED    28336932
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-23"; volume: "7(1):337. doi"; pages: " 
            10.1038/s41598-017-00407-1"
COMMENT     Sequence Label: pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2630..2641
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5570..5635
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        5677..5888
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5901..6169
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6216..6236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6261..6923
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    7013..7601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7066..7086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7484..7495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7675..7908
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7980..8101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8017..8036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8071..8090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8141..8276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8309..8325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8309..8325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8322..8344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8333..8349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8357..8363,8364..8381,8382..8387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8402..8423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8540..8557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8711..9299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9470..10261,10262..10330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10093..10112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10331..10435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10503..10521
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10559..10581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10681..10700
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10894..10916
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10908..10925
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10909..10925
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gccaccatgg ggacctccca ccaggtcttt ctggtcctca gctgtctcct
     2341 cacagggccg ggcctcatct cctgccagct cttattaccc tctatcctcc caaacgagaa
     2401 tgagaagatt gtgcagctga attcgtcctt ttctctgaga tgcgttgggg agagtgaagt
     2461 gagctggcag caccccatgt ctgaagaaga cgaccccaac gtggaaatca gaagtgagga
     2521 gaacaacagt ggcctctttg tcacagtgct ggaagtggtt aacgcctccg cagcccacac
     2581 cggatggtac acttgctact acaaccacac tcagacggat gagagtgaga tcgaaggcag
     2641 gcacatttac atctatgtac cagacccaga catggccttt gtgcctctcg ggatgaccga
     2701 ttccttagtc atcgtggaag aggatgactc tgccatcata ccttgccgca ccacagatcc
     2761 ggagactcaa gtaaccttgc acaataacgg gaggctggtg cctgcctcct atgacagcag
     2821 gcagggcttc aacggaacct tcagcgtggg gccttacatc tgtgaggcca ccgtcaaagg
     2881 gaggacgttc aagaccagcg agtttaatgt ttatgccttg aaagcaacgt cagaactgaa
     2941 tctggagatg gacgcacgcc agactgtgta taaggcagga gaaacgatcg tggtgacctg
     3001 tgccgtcttt aacaacgagg tcgttgacct gcagtggact taccctggag aagtgagaaa
     3061 caaaggtatc accatgctgg aggagatcaa actcccatcc atcaaactgg tgtacacttt
     3121 gaccgtcccc aaggccacgg tgaaggacag tggagagtat gaatgtgctg cccgtcaggc
     3181 cactaaagag gtcaaggaaa tgaagagagt caccatttct gtccacgaga aaggcttcgt
     3241 cgagattgag cccacctttg gccagctgga agctgtcaac ttgcacgaag tcagagagtt
     3301 cgtggtggag gtgcaggcct acccgacgcc caggatatcg tggctgaagg acaacttgac
     3361 tctgatagag aatctcaccg agatcaccac tgatgtacag aagagtcagg aaacaaggta
     3421 tcagagcaaa ttaaagctga tccgggctaa ggaagaagac agtggccatt acaccattat
     3481 agttcaaaat gaggatgacg tgaagagtta cacgtttgag ctgtcaaccc tagttcctgc
     3541 atccattttg gatctcgtag atgaccacca tggctctggc gggggacaga ctgtgaggtg
     3601 tacagccgaa ggcacccctc ttccagaaat cgactggatg atctgcaagc atattaagaa
     3661 atgtaataat gacacttcgt ggacagtttt ggccagcaat gtctcaaata ttatcacaga
     3721 gctccctcgc cgtggcagga gtaccgtgga gggacgagtg tccttcgcca aagtggaaga
     3781 gaccatcgca gttcgatgcc tggcaaagaa caacctcagc gttgtggccc gtgagctgaa
     3841 gctggtggct cccactctgc gatctgaact cacagtggcg gccgcagtgt tggtgctgtt
     3901 ggtgattgtc attgtctctc tcatcgtcct ggtggtcatt tggaagcaga aaccacggta
     3961 tgaaattcgc tggagggtta tcgagtcaat cagccccgat ggacatgagt atatctatgt
     4021 ggaccccatg cagttgcctt acgactccag atgggaattc ccgagagatg gcctcgtgct
     4081 tggtcggatt ttgggatccg gtgcatttgg gaaagtggtc gaaggtacag cttatggatt
     4141 aagccggtcc caacctgtaa tgaaggtggc tgtgaagatg ctcaaaccca cagccaggtc
     4201 tagtgaaaag caggctctca tgtctgagct gaagataatg actcacctgg ggccacattt
     4261 gaacattgtg aacttgctgg gagcctgcac caagtcaggt cccatttaca tcatcacaga
     4321 atactgcttc tatggggatt tggtcaacta cttgcataag aacagagata gcttcatgag
     4381 ccaacaccca gagaagccga agaaagacct ggacatcttt ggattgaatc ctgcagacga
     4441 gagcacaaga agttatgtga ttttgtcttt tgaaaataac ggcgactaca tggacatgaa
     4501 gcaagctgat accacacagt atgtccccat gcttgaaagg aaagaggttt ctaaatactc
     4561 tgacatccag agatcgctgt acgatcggcc agcctcctac aagaagaaat ccatgctaga
     4621 ctcagaagtc aaaaacctcc tttcggacga tgactccgag ggtctgactt tgctggatct
     4681 attgagcttc acctatcaag ttgctcgagg aatggaattt ttggcttcga aaaattgtgt
     4741 ccaccgggac ctggctgccc gcaacgtcct cctggcgcaa ggaaaaattg tgaagatctg
     4801 tgattttggc ctggccagag acatcatgca cgattccaac tacgtgtcaa aaggcagtac
     4861 tttcctgcct gtgaagtgga tggcacccga gagcatcttc gacaacctct acaccacgct
     4921 gagtgacgtc tggtcctatg gcattctgct ctgggaaatc ttttcccttg gtggcacacc
     4981 ctatcctggc atgatggtcg attctacttt ctacaataag atcaagagtg gataccggat
     5041 ggccaaacct gaccatgcca ccagtgaagt ctatgagatc atggtgcagt gctggaacag
     5101 tgagcccgag aagagaccct ccttctacca cctcagcgag atagtggaga acctgttgcc
     5161 gggacaatac aagaagagtt atgaaaagat tcacctggac ttcctaaaga gtgaccatcc
     5221 ggccgtggca cgcatgcggg tggactctga taatgcgtac atcggtgtca cttacaaaaa
     5281 tgaagaggat aagctgaagg actgggaagg tggcctggac gaacagagac tgagcgctga
     5341 cagtggctac atcatccccc tgccagacat tgaccctgtt ccagaggagg aagacctggg
     5401 caagaggaac agacacagct cacagacttc ggaagagagt gccatcgaga caggttccag
     5461 tagttccacc ttcatcaaga gagaggacga gaccatcgag gacatcgaca tgatggatga
     5521 cattggtata gattcctcgg acctggtgga ggacagcttc ctgcgtacgg actacaagga
     5581 ccatgatggt gattataagg atcacgacat cgactacaag gatgacgatg acaagtaagg
     5641 tacgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     5701 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     5761 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     5821 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     5881 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     5941 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     6001 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     6061 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     6121 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     6181 cggcgcctac gctagacgcc acctaggcca ccatgcccaa gaagaagagg aaggtgatgg
     6241 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga
     6301 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga
     6361 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg
     6421 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc
     6481 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg
     6541 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca
     6601 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca
     6661 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg
     6721 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc
     6781 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc
     6841 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct
     6901 tcaagacccc cattgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg
     6961 gcaccgccgg acccggctcc accggatctc gctaatgtac aagtgagtcg acaatcaacc
     7021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7381 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7561 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     7621 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7681 ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt
     7741 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7801 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7861 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7921 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7981 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8041 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8101 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     8281 cttttgcaga gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     8341 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8401 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8461 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8521 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8581 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8641 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8701 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8761 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8821 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8881 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8941 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9001 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9061 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9121 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9481 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9541 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9601 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9661 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9721 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9781 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9841 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9901 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9961 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10021 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10081 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10141 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10201 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10261 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10321 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10381 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10441 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10501 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10561 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10681 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10741 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10801 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10861 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10921 ccagtgccaa gctg
//
