LOCUS       sequence-196629-        8754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of Tmeff2 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M
  TITLE     Tmeff2 is expressed in differentiating oligodendrocytes but 
            dispensable for their differentiation in vivo.
  JOURNAL   Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1.
  PUBMED    28336932
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-23"; volume: "7(1):337. doi"; pages: " 
            10.1038/s41598-017-00407-1"
COMMENT     Sequence Label: pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
FEATURES             Location/Qualifiers
     source          1..8754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             2423..2431
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3419..3448
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3449..3478
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3520..3731
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3744..4012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4051..4060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4081..4743
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    4833..5421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4886..4906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5304..5315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5495..5728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5800..5921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5837..5856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5891..5910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5961..6096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6129..6145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6129..6145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6142..6164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6153..6169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6177..6183,6184..6201,6202..6207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6222..6243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6360..6377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6531..7119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7290..8081,8082..8150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7913..7932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8151..8255)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8323..8341
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8379..8401)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8501..8520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8714..8736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8728..8745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8729..8745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gccaccatgg tgctgtggga gtccccgcgg cagtgcagca gctggacact
     2341 ttgcgagggc ttctgctggc tgctgctgct gcccgtgacg ctgcttatca tagcccgccc
     2401 ggtaaaactc gctgctttcc ctacctcctt aagtgactgc caaacgccca ccggctggaa
     2461 ctgctccggt tacgatgaca gagaaaatga tctcttcctg tgtgacacca acacctgtaa
     2521 atttgacggg gaatgtttaa ggattggaga cactgtgact tgcgtctgtc agttcaagtg
     2581 taacagtgac tacgtgcctg tgtgtggctc caatggagag agctaccaga atgagtgcta
     2641 cctgaggcaa gctgcctgca aacagcagag tgagatactt gtggtctcag aaggatcctg
     2701 tgctacagat acaggatcgg gatctggaga tggagtccat gaaggctctg gcgagaccag
     2761 tcagaaggag acatccacct gtgacatttg ccagtttggg gcagagtgtg atgaagatgc
     2821 tgaggatgtc tggtgtgtgt gcaacataga ctgttctcaa accaacttca atcccctctg
     2881 tgcttctgat gggaaatctt atgataatgc atgtcaaatc aaagaagcgt catgtcagaa
     2941 acaggagaaa attgaagtca tgtctctggg tcgatgtcaa gataacacaa ctacaaccac
     3001 taaatctgaa gatgggcatt atgcaagaac agattatgca gagaatgcta acaaattaga
     3061 agaaagtgcc agagaacacc acataccttg cccagaacat tataatggtt tctgcatgca
     3121 tgggaagtgt gaacactcca tcaatatgca ggagccatct tgcagatgcg atgctggtta
     3181 cactggtcaa cactgtgaaa agaaggacta cagtgttctc tacgttgttc ctggccctgt
     3241 ccggttccag tacgtcttga ttgcagctgt gattggaaca attcagattg ctgtcatctg
     3301 cgtggtggtc ctctgcatca caaggaaatg ccccagaagc aacagaattc acagacagaa
     3361 gcaaaataca gggcactaca gttcagacaa cacaacaaga gcatccacac ggttaatcga
     3421 acagaaactg atctctgaag aagacctgga gcaaaagctc atatcggagg aggatctcta
     3481 aggtacgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3541 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     3601 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3661 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3721 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     3781 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     3841 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     3901 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     3961 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     4021 gaccggcgcc tacgctagac gccacctagg gccaccatgg agagcgacga gagcggcctg
     4081 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4141 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     4201 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     4261 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     4321 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     4381 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     4441 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     4501 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     4561 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     4621 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     4681 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc cattgccttc
     4741 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     4801 accggatctc gctaatgtac aagtgagtcg acaatcaacc tctggattac aaaatttgtg
     4861 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4921 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4981 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5041 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5101 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5161 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5221 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5281 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5341 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5401 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     5461 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5521 agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5581 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5641 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5701 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5761 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5821 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5881 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     5941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6061 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc
     6121 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8281 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8341 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8401 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8461 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8521 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8581 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8641 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8701 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
