LOCUS       sequence-196648-        9331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNAP47 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan JC, Nunez 
            M, Moncion T, Loew D, Formstecher E, Galli T, Proux-Gillardeaux V
  TITLE     The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein 
            Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected 
            Vesicle-associated Membrane Proteins (VAMPs).
  JOURNAL   J Biol Chem. 2015 Nov 20;290(47):28056-69. doi: 
            10.1074/jbc.M115.666362. Epub 2015 Sep 10.
  PUBMED    26359495
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.666362"; journalName: "J Biol Chem"; date: 
            "2015-11-20- 20"; volume: "290"; issue: "47"; pages: "28056-69"
COMMENT     Sequence Label: pEGFP SNAP47
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
ORIGIN
        1 aaaagacaga aaaactacaa gtaccggtag aagcaaagat aaaaacaaat actctgaaag
       61 aaaaccaaac agtaaaaaag ggcgaagagt tagtcacttt tgaaacaacc cctttgcaaa
      121 acgaaaaaaa acaattagag caagaaaaca caacgattga ggaacaaaaa aaagcggcac
      181 aaacttttat tgacagccta acaaatgaac aaaatttatt tgagacagaa gattccttcg
      241 gctatagcaa tcaagtaaag agttttctgg ctgaaaaaga ggcaacgcgc tatgcctcaa
      301 agcaaagtga aacaaacaca caaaaggagc aagaagcgta caacaaaacc aaagaacaac
      361 tagaaaaaca actaactacg cgtcaaaaag aacaaaagga atgggagcag gtacgcacgg
      421 catggatcaa tgaacaatcc ctagagggat tttcaactga aatcacatcc aggtatcagg
      481 cgtggcaatc tcaattaacc gacgtaccag aagaacaaaa gaaacaaatt aaagcaacga
      541 cattggctac aatcgacgaa caaatcagtc aggtaaaaaa agaaatcgag caacttcaaa
      601 gcgaacaatc aaagctagta gcgccaacca cgtctgataa tgaagtaagt agtcaaacag
      661 aaaaaacaaa gcaaaccaaa gaactagcct tggcaacagc gaaacaaaaa gtggtagaat
      721 taaccgagac ccaaaagaaa aatgagacag cactgaaaac aatcaacgaa caactcgcac
      781 aaagtacgtt gaaagcacct atcacaggaa ctgttcacgt aaatgaggaa gtcaaaaacc
      841 aattggaagt cccgaaaggc acaacgttgg cagaaattta tccgaaatca aaagacccac
      901 aacttacgtt taccgcactt attcctacga gtgaaagcat gagaatcaaa cctggaatgg
      961 caatacattt caaactggat aagaaaggag tagcaactaa aacaatcgac ggaacgctaa
     1021 cggaaatttc agaaaacagt acgacaactg aacaaggagc tttttataca ataaaaggca
     1081 cactaaaacc tccaaaagag tttaccggtc gctatggtct tacaggggag ctttctctca
     1141 ttgttggaaa gaaaacctat tggcaacaaa tcaaagatat gttattaaat caagaataga
     1201 aaaagagtga gggattatca tgaatgagtt agaaaaacta caaatccaag aacaaaaact
     1261 acgagaaaag aaaaagcaac tactcgcaaa ggaatacagc cgtttaggac gtgcctttta
     1321 caaaaaagcg aaagtgaaat cctttgacga agcccaacaa gccgtcacca attatcaacc
     1381 aggaacactc ggcataacaa acgatcaagt agaaaaatta aaacagtttg ctaataacct
     1441 aacctggaat gaatcaggca actattggta tacgaatgag gtcaaagaat taacacaatt
     1501 tttggcacaa ttccgcgagg aagtgtaagt cgattgaacg ggtttagttg cttcgataat
     1561 ccgaagcaac ttttcctcca tttatggagg aaaaaagggg cgttttcgaa cagaaaaccg
     1621 accctgaaaa cgttggagaa aaatcgccgt acaggcgacc tttttcaagc aacgttcggg
     1681 ggattggggg atacccccaa caagctattt gtagtacgaa gtgctagcaa ataaactgct
     1741 tgccaaaacc tcaggatggg cacataatga ggttgcctgt tttgttggta agtggtgact
     1801 gactaaggaa caacgaacct acaaaactta gaggaaaggt gtaaccgaat agggcacaac
     1861 ttgacgtttt ctcttgtgag agttgtgagt gacaaacgaa caattggcac tagagaaagg
     1921 ctaaagaaaa ttgaccgaat agggaatttt tctggcgcga tcttttgatt ttttttcgta
     1981 gccgtaggca cgaaaacaga caaagggaaa cgactgaaga aggagttttc tctgtctgct
     2041 tggggtgaga ttgtcgaaca gacaactcgc aacccaagtg acggaacaaa tcggcgaaca
     2101 gccgactttg agaaggaaag gagcgaacac aatgacagaa gaaaaagagc aaggagtgaa
     2161 ccggaaatcc cccaaaaagc gccactatac gcaagtgaat tttcgtttaa ctgaagccga
     2221 gaaagaacgc tggcaaaaac aagccagtag cctgaacatg accttaccaa agttctcaaa
     2281 acacttgatt aatttattct ttgattctgg gacaatcaag caaccaaaaa ttgacaaaca
     2341 acaaggaacg gaaattgccc gccacttagc caaactaggc ggaaatgtga atcaaatcgc
     2401 caagtggtgc aatacccatc aacacgacat ttcagacgtt caggctaaac ggttagccga
     2461 gaacttagca cacgtgcaaa aggagttgaa acaggtatgg caacaattaa actagcgcgt
     2521 accaccagtt gcaatcgggc cattagctat gcggaaaagc gcgccgtgga aaaagacggc
     2581 gtcaactgcg aaatcaccaa cgccaagcaa gaaatggcca cgattcgcca actcttctca
     2641 aaaaccgata aaacacaagc ccatttgtgt atccaatcct ttagcccaca agagagccaa
     2701 caacttgggc caaagaaaat caatcagtta ggcatagaat tggccgagaa aatggccccc
     2761 aaccatcaag tagcggtcta tacccacgcc gacacagacc atatccataa ccatctggtg
     2821 atcaatgcgg tgaatttaga aaccggcaac aaatacagcc accataacga ctttcagcgc
     2881 gtatctgccc tccatgacca gctattgcag gaacaccagt tacagatcgt cgaaaaacaa
     2941 gcggtggaac gtcggaccat ggccgaacga aaactcgccg aaaaagggca atgggtttgg
     3001 aaagaccaaa tcaggcagac aatcgacgcc ttgatgaagg atagctctat aagctcatat
     3061 aaggcgttta gagagggttt ggatcaaaga ggtataaaat tacatgaacg aggtcaaaac
     3121 gtcgtatacg agcttttaga ggggaataag agggttcgcg gagcgaagct aggaacagat
     3181 tatgaaaagg acgtgattaa acatgagttg gaccaccgag aacaaacacg aacaattgaa
     3241 acacaagcaa gaacgcttgc aaatggactt agaacgagcg attttagtcg aggaagtgac
     3301 gaaattacgc acgatccaag aagagattcc ccaattgatt tcagaatggg aacagcaatt
     3361 tcagacctta agcaccaact tcgacaagca acagaaacac catcagaacg aactgagcga
     3421 aaaaatcgag aacgagttga agcacttaaa cagacggata caagaccaat tagacacact
     3481 catcagccag aaccagatct tacaccacga caatcaggtc cttctctcgg actatgaaaa
     3541 ctacctggac aaacatgtca aagaaatcaa tcaagaatac cgcctacttg tcgaacacca
     3601 agcccttgca caggtgagcc atttgaaaga actgacagca aacctggaaa aagcacaaga
     3661 agaagtgcaa gaagcgatgg gtatgttcgc tagaaaacag ttagcccagg caaaaacagt
     3721 gaataacatc aaagacgtgg ccatgagtgc cgtttttgtc ttgattacac tcgctgttct
     3781 ttggacagtg ggctatctat taggcacgtt tttccagttg gacgaatttt atcggtggtt
     3841 agttgcctca accattggca aaggcgttct ctgggggcta gggcttgcac tcctagttgg
     3901 atcaattggt agtgtggttt attcggtaaa gaactaatgg tctttccagg gtcgttaggg
     3961 cttgccctga ccgtctgaaa gacgcaaaca aggaaaacta tcaaggaatg cttaactcgt
     4021 cttagaaagg ctgacagtgg cagttaggac gatcccacag gtctccttgt tgtgggtttg
     4081 cttttagcga gtgaccgaaa gggaacgctg caagtacaat gtgggagcaa agcgagcact
     4141 ccttttagcg aacaacgtga gcgaagcatt tccagtttat tgattacgcg aaccagtttt
     4201 tagaaaaaag aggtcttttt taggcgggaa tttttgcccc cctcttttat aaactctttt
     4261 taaacctctt ttaaacctct ttaccgtcgg ctgtactcca cgccctcaaa ggagtttatc
     4321 gttttatgaa cgtagttttg tctgtttatg aacgtagttt tgtctgttta tgaacgtagt
     4381 tttgtctgtt tatgaacgta gttttgtctg tttagaagtt tctttataaa cgtattacgt
     4441 tttacaaata cgtttcaata tggtagtctt ttcttcaaga ggtgactata tatggcaaac
     4501 gaacttgtta agtatcattc agaattaaat accattcctt tgaggaaatt tactcctgta
     4561 gaaatgaact tgttcttctc tattgtttct agaatgcgtg aaaaaggcga caagacagtt
     4621 cgttttactt ttgaccaatt aaaagaatta agcaattata aagcgactgc taatatgcgt
     4681 ttcattgatg atttagaaga aacatataaa aaaattttag agttgcgatt cggcaggaga
     4741 agtcaaagtg gcttaactag agaatttttt gtgatgttta ctaaattcaa aattaacggt
     4801 gatactgacg aaccttatgt agatattcaa attttcgaag atgcacttcc tctgttaaat
     4861 gacttagatt cttgggttcg ctactcatta caacaattca atgaactaca aagtagttat
     4921 tctaaaacca tgtttcgttt attgaaacaa tttcgtacca aaggatatgc gtatttctct
     4981 aaagaagact ttcaagaatt gttagatatt ccaaaaagct ataaacaagg agatattgat
     5041 agaactgttt tacagcctat taaacaagaa cttactgcta tcttcaaagg tttatcaatt
     5101 aaaaagaaat atggaagagg tcgcggtaaa cccgttattg gttatcaatt cacgtttaaa
     5161 cccgagatca aaaatgctaa tgatttcaat aaacatgccc ctactaaaat cgaacagagt
     5221 caggcgttat ctcaggtaaa cgggcaaaaa aattatgaag aacaactaac aactttggac
     5281 gataagatta aagaacaaga gcgtagatta gacttattaa aacaactcaa agaattagag
     5341 caaaataaaa aagacgagaa aagcttgcaa atacagaccg agaataaaga tagatttact
     5401 ctttcaaaaa gggataaaaa agatatactt agcggtttac aaaatttcaa taaattatag
     5461 taagaagcac atagtttagc agcaatataa atatattaaa ccaactatat caaatatcta
     5521 ttgaatacct taaataaatt tattttcaat actttacttt tggagggatt gctatgttat
     5581 ttttactaaa atttattacg ctattaagca ttatttctta tttatatcta tgtattatgt
     5641 atatgaaaaa tatggacaaa tataaattat tagcattaat acttataggg ggctgcattt
     5701 tattatctat cgttctaagt atatttaact tatcgtttat cttaattaga ctgtctctat
     5761 tacttggatt atctggcctt ttactattgc ttaaaccagc cattaaaaat acatttaaag
     5821 atgatttttc atttttagaa ggtatagcat taagtgctat aacatcaagt tacttagtaa
     5881 gcttaatttc attatttata cggtcttaat tattattgtg cgtttttcta aaaggtaata
     5941 caaatataat aaacctgtgg tgctagttga tttttcttaa aatcaactag caccacaggt
     6001 ttattattac gttaaaggaa agagcccata atatattaat ttacttaatt aaatagttca
     6061 gtacaatgta tatacattga gtaaaaaaga ataacacaaa cattgtttga tttttttttg
     6121 ttaaaactga tttctttttt aaaattatat ataaatatat aatagtaatt atgctcaaca
     6181 attctaacaa attagttaac agacttaaat tgaaaggtac tatcatagta aaaaccatta
     6241 gaatgaatac gctaaagaaa aatgtataag ctttttttat tgcatgatct gaataattga
     6301 tatcaattcc aattaaaatg tataaaaatg ttataaaatt aaaaaaggtt atgatgctta
     6361 taaaaatatc catatttatc tccttaaaaa taataaaaac atatggcgtt aacgccatat
     6421 gtttttatta tactctttaa ttatacattc ttccacttat ttttatttcc ttcttttcca
     6481 attccttttg caagacctgt aacaaaatca tatgcgggtt gtagccatgg taaccatttt
     6541 ccaggtcctc caccagtaat tgctttcaac tcattatctt caattacttt tatattactt
     6601 gcattcttaa tgtttttcat attagcatat ctcctctatt tgatttcgat tcttaatgtt
     6661 tctttctatt aattctatca gcttggttta catcactcat attacctaat ccttttaaaa
     6721 tccaataagc agctggacct acagcattac ctattttact aaaaaccgac tctcccccaa
     6781 tagttttttt catttctttt tcattcaata ctttatattg cttcatttat cgttccccct
     6841 aattattttt gtaatataat tctggaatta tattacttaa tttattaaat caaagtcttt
     6901 ttattatgtc aattaactat ataaaaaggt tttcaaaaat attttattat agtaatattg
     6961 ataaatgaat aataacacaa gtgaggagaa actttgaaaa agattattta ccaacaagac
     7021 gaaaaagatt gtggcgtagc ttgtattgcc atgattttaa agcattataa tacagaaata
     7081 cccattcaca agttacgtga gttgtctgga acagatttag agggaacttc cgcttttggt
     7141 ctaaaaagaa ctttagaaaa aattggtttt gattctcctg ctattcaagc tgataatgaa
     7201 gtatggaaag aaaaagaatt accgttacct ttaattgcgc atgtcttaat tgacaataaa
     7261 tacatgcact atgtcgtggt ttatggaata aaaggagata ccttattaat tgctgatcca
     7321 gatccaacag taggaaaagt aaaaaaatca atggaagaat tttccaaaga atggacaggc
     7381 gttttactgt tacctacacc taaagagtct tatactccta ccaaagaaaa agtcgctgga
     7441 ttgagctctt ttttacccat tatttggcgt caaaaaggct tagtttttca cattatttta
     7501 gcgtctcttt ttattacgct atttgggatt gggagctcct actattttca aggaatttta
     7561 gattacttta ttcctaatca agcacgttct actttaaaca ttgtttcgat tggcttgatt
     7621 gctgtctatc tgttccgtgt cctgtttgaa tacagtcgga gttatctctt agttatccta
     7681 ggacaacgca tgagcatggc cgtaatgctt caatacttta aacatgttct tactttacct
     7741 atgaactttt ttgctacccg taaatcaggg gagatcattt ctcgttttct agacgcaaat
     7801 aaaatcattg atgccctcgc cagtgcaacc ttatccgtct ttttagacat tgggatggtc
     7861 ttcttagtag ggattacctt agccattcaa aatggtacat tattcttaat taccttagct
     7921 tcgttaccgt tttattttgt ggccattcta gcctttgtca aaagttatga aaaagccaat
     7981 caagaagaaa tggcggctgg cgctacctta aattcaagta ttatcgagag cctaaaagga
     8041 atcgagacga tcaaggccta cagtggagaa gaaaaagtct ataatcgcgt ggatcgagaa
     8101 tttattcgat taatgaaaaa gtccttccgg actgttacgc tagataatgt gcaacaaggg
     8161 attaaacatg ctattcagtt aatcagtagt gcccttattt tgtggctagg ttctttctat
     8221 gtcatggatg gtacgattag ccttggccaa ttgattactt acaatgcctt gttggtgttt
     8281 ttcacggatc ctttacagaa tattattaat ctacaagtca aaatgcaaac agcgcaagtg
     8341 gccaacaaac gcttaaacga gatttttgcc attgaaccag aacaaacaaa cgattcttcg
     8401 gaacgcgaaa ttccaaatca aacgttccaa caagaaataa gactagaaaa tgtctccttc
     8461 tcctataaca tgaaagcacc taccttaaac aatatttctt gcaccattgc accgaaaagt
     8521 aaaatcgcct tggttggggt aagtggttca gggaaatcaa cattagcaaa attattagtg
     8581 aacttttatg cgccttcaga aggaaccatt cgctacggaa aaatgaatca tttagatatt
     8641 ccgtttcaac aattacgtga acacgtcacc tatgtgccgc aagagtcttt tttctttagc
     8701 gggacaattg tagaaaacct aacgtttggc ttacagaaca tgcctagttt tgaacggatt
     8761 attgaagtgt gtgaagccgt tcaactaaac acattcattg aacaacagcc tttacgctat
     8821 gacatggtac tagaagaagg aggggcgaat ctttcaggag gacaacggca acgattggcg
     8881 attgctcgtg ctcttttgaa agacgcggat gttttaatct tagacgaagc aactagcgga
     8941 ttagacacac tattagaaca tgcgattctt gattatttac taacgttaga agagaaaaca
     9001 ttgcttttta ttgcgcacca tttgcccatt gctaaagcgt gcgatcacat tctagttctt
     9061 catgaagggc aactggttga acaaggggct catgacgacc tccgttataa tcagggggtt
     9121 tatcaacagt tatgggatat ttaggaaagg acagaaaaat tgatgcaaca aaaggattgg
     9181 ctcgatcatt cgagtgttta tagccaacag caccacacct tctatcgatg ggtcttgtat
     9241 cctgtcattc tttttttgtg cttaggggcc ttatttttag cttttgcgaa aaaagaagtc
     9301 gtgattcgaa cacctgcaca actaacggca c
//
