LOCUS       sequence-196687-         513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  'Localizer' construct that marks tetO arrays and is targeted by a 
            PHR-tagged effector upon illumination with blue light; can be 
            visualized without triggering PHR recruitment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pCIBN-TetR-tagRFP-T
FEATURES             Location/Qualifiers
     source          1..513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..510
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 atgaatggag ctataggagg tgaccttttg ctcaattttc ctgacatgtc ggtcctagag
       61 cgccaaaggg ctcacctcaa gtacctcaat cccacctttg attctcctct cgccggcttc
      121 tttgccgatt cttcaatgat taccggcggc gagatggaca gctatctttc gactgccggt
      181 ttgaatcttc cgatgatgta cggtgagacg acggtggaag gtgattcaag actctcaatt
      241 tcgccggaaa cgacgcttgg gactggaaat ttcaaggcag cgaagtttga tacagagact
      301 aaggattgta atgaggcggc gaagaagatg acgatgaaca gagatgacct agtagaagaa
      361 ggagaagaag agaagtcgaa aataacagag caaaacaatg ggagcacaaa aagcatcaag
      421 aagatgaaac acaaagccaa gaaagaagag aacaatttct ctaatgattc atctaaagtg
      481 acgaaggaat tggagaaaac ggattatatt cat
//
