LOCUS       sequence-196691-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  'Localizer' construct that marks lacO arrays and is targeted by a 
            PHR-tagged effector upon illumination with blue light; can be 
            visualized without triggering PHR recruitment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pCIBN-LacI-tagRFP-T
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..42)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atggtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
       61 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
      121 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
      181 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
      241 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
      301 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
      361 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
      421 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
      481 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
      541 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
      601 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
      661 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
      721 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
      781 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
      841 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
      901 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
      961 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     1021 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccga
//
