LOCUS       sequence-196730-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Soluble BLInCR transcription activator (transactivation domain of 
            viral VP16 protein) that is recruited to 'localizer' sites upon blue
            light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-iRFP713-NLS-VP16
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..945
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVME"
ORIGIN
        1 atggctgaag gatccgtcgc caggcagcct gacctcttga cctgcgacga tgagccgatc
       61 catatccccg gtgccatcca accgcatgga ctgctgctcg ccctcgccgc cgacatgacg
      121 atcgttgccg gcagcgacaa ccttcccgaa ctcaccggac tggcgatcgg cgccctgatc
      181 ggccgctctg cggccgatgt cttcgactcg gagacgcaca accgtctgac gatcgccttg
      241 gccgagcccg gggcggccgt cggagcaccg atcactgtcg gcttcacgat gcgaaaggac
      301 gcaggcttca tcggctcctg gcatcgccat gatcagctca tcttcctcga gctcgagcct
      361 ccccagcggg acgtcgccga gccgcaggcg ttcttccgcc gcaccaacag cgccatccgc
      421 cgcctgcagg ccgccgaaac cttggaaagc gcctgcgccg ccgcggcgca agaggtgcgg
      481 aagattaccg gcttcgatcg ggtgatgatc tatcgcttcg cctccgactt cagcggcgaa
      541 gtgatcgcag aggatcggtg cgccgaggtc gagtcaaaac taggcctgca ctatcctgcc
      601 tcaaccgtgc cggcgcaggc ccgtcggctc tataccatca acccggtacg gatcattccc
      661 gatatcaatt atcggccggt gccggtcacc ccagacctca atccggtcac cgggcggccg
      721 attgatctta gcttcgccat cctgcgcagc gtctcgcccg tccatctgga attcatgcgc
      781 aacataggca tgcacggcac gatgtcgatc tcgattttgc gcggcgagcg actgtgggga
      841 ttgatcgttt gccatcaccg aacgccgtac tacgtcgatc tcgatggccg ccaagcctgc
      901 gagctagtcg cccaggttct ggcctggcag atcggcgtga tggaa
//
