LOCUS       sequence-196740-        8711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hGCN5-NLS
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             625..2118
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2128..2130,2131..2133,2134..2844)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2173..2194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2434..2453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2781..2802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5365..5385
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5499..5620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5536..5555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5590..5609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5627..6082)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5764..5785)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5976..5995
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6109..6213
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6215..6572
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6215..6406
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6238..6258)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6423..6558
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6607..7401
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6607..7401
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6661..6680)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7271..7290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7589..7608)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7633..7680
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8009..8597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8498..8517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgaag atggacaaaa agactatagt ttggtttaga
      661 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
      721 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
      781 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
      841 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
      901 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
      961 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     1021 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     1081 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     1141 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     1201 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     1261 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     1321 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     1381 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     1441 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     1501 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     1561 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     1621 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     1681 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     1741 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     1801 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     1861 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     1921 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     1981 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     2041 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
     2101 atcatgatcg gagcagcagc ccgggtcatg gtgagcaagg gcgaggagct gttcaccggg
     2161 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2221 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2281 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     2341 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2401 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2461 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2521 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2581 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2641 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2701 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     2761 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2821 ctcggcatgg acgagctgta caagagcggc cgcatggcgg aaccttccca ggccccgacc
     2881 ccggccccgg ctgcgcagcc ccggcccctt cagtccccag cccctgcccc aactccgact
     2941 cctgcaccca gcccggcttc agccccgatt ccgactccca ccccggcacc agcccctgcc
     3001 ccagctgcag ccccagccgg cagcacaggg actggggggc ccggggtagg aagtgggggg
     3061 gccgggagcg ggggggatcc ggctcgacct ggcctgagcc agcagcagcg cgccagtcag
     3121 aggaaggcgc aagtccgggg gctgccgcgc gccaagaagc ttgagaagct aggggtcttc
     3181 tcggcttgca aggccaatga aacctgtaag tgtaatggct ggaaaaaccc caagcccccc
     3241 actgcacccc gcatggatct gcagcagcca gctgccaacc tgagtgagct gtgccgcagt
     3301 tgtgagcacc ccttggctga ccacgtatcc cacttggaga atgtgtcaga ggatgagata
     3361 aaccgactgc tggggatggt ggtggatgtg gagaatctct tcatgtctgt tcacaaggga
     3421 gaggacacag acaccaatca ggtctatttc tacctcttca agctactgcg gaaatgcatc
     3481 ctgcagatga cccggcctgt ggtggagggg tccctgggca gccctccatt tgagaaacct
     3541 aatattgagc agggtgtgct gaactttgtg cagtacaaat ttagtcacct ggctccccgg
     3601 gagcggcaga cgatgttcga gctctcaaag atgttcttgc tctgccttaa ctactggaag
     3661 cttgagacac ctgcccagtt tcggcagagg tctcaggctg aggacgtggc tacctacaag
     3721 gtcaattaca ccagatggct ctgttactgc cacgtgcccc agagctgtga tagcctcccc
     3781 cgctacgaaa ccactcatgt ctttgggcga agccttctcc ggtccatttt caccgttacc
     3841 cgccggcagc tgctggaaaa gttccgagtg gagaaggaca aattggtgcc cgagaagagg
     3901 accctcatcc tcactcactt ccccaaattc ctgtccatgc tggaggagga gatctatggg
     3961 gcaaactctc caatctggga gtcaggcttc accatgccac cctcagaggg gacacagctg
     4021 gttccccggc cagcttcagt cagtgcagcg gttgttccca gcacccccat cttcagcccc
     4081 agcatgggtg ggggcagcaa cagctccctg agtctggatt ctgcaggggc cgagcctatg
     4141 ccaggcgaga agaggacgct cccagagaac ctgaccctgg aggatgccaa gcggctccgt
     4201 gtgatgggtg acatccccat ggagctggtc aatgaggtca tgctgaccat cactgaccct
     4261 gctgccatgc tggggcctga gacgagcctg ctttcggcca atgcggcccg ggatgagaca
     4321 gcccgcctgg aggagcgccg cggcatcatc gagttccatg tcatcggcaa ctcactgacg
     4381 cccaaggcca accggcgggt gttgctgtgg ctcgtggggc tgcagaatgt cttttcccac
     4441 cagctgccgc gcatgcctaa ggagtatatc gcccgcctcg tctttgaccc gaagcacaag
     4501 actctggcct tgatcaagga tgggcgggtc atcggtggca tctgcttccg catgtttccc
     4561 acccagggct tcacggagat tgtcttctgt gctgtcacct cgaatgagca ggtcaagggt
     4621 tatgggaccc acctgatgaa ccacctgaag gagtatcaca tcaagcacaa cattctctac
     4681 ttcctcacct acgccgacga gtacgccatc ggctacttca aaaagcaggg tttctccaag
     4741 gacatcaagg tgcccaagag ccgctacctg ggctacatca aggactacga gggagcgacg
     4801 ctgatggagt gtgagctgaa tccccgcatc ccctacacgg agctgtccca catcatcaag
     4861 aagcagaaag agatcatcaa gaagctgatt gagcgcaaac aggcccagat ccgcaaggtc
     4921 tacccggggc tcagctgctt caaggagggc gtgaggcaga tccctgtgga gagcgttcct
     4981 ggcattcgag agacaggctg gaagccattg gggaaggaga aggggaagga gctgaaggac
     5041 cccgaccagc tctacacaac cctcaaaaac ctgctggccc aaatcaagtc tcaccccagt
     5101 gcctggccct tcatggagcc tgtgaagaag tcggaggccc ctgactacta cgaggtcatc
     5161 cgcttcccca ttgacctgaa gaccatgact gagcggctgc gaagccgcta ctacgtgacc
     5221 cggaagctct ttgtggccga cctgcagcgg gtcatcgcca actgtcgcga gtacaacccc
     5281 ccggacagcg agtactgccg ctgtgccagc gccctggaga agttcttcta cttcaagctc
     5341 aaggagggag gcctcattga caagccaaaa aagaagagaa aggtatagtc tagatcataa
     5401 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5461 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5581 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     5641 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5701 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5761 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5821 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5881 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5941 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6001 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6061 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6121 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6181 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     6241 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6361 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6421 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6481 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6541 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     6601 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6661 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6721 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6781 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6841 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6901 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6961 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7021 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7081 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     7141 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7201 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7261 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7321 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7381 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     7441 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7501 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7561 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     7621 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     7681 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     7741 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     7801 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     7861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8641 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8701 ccgccatgca t
//
