LOCUS       sequence-196743-        8690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble BLInCR effector that is recruited to 'localizer' sites upon 
            blue light illumination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pPHR-EYFP-hGCN5mut
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             625..2118
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             join(2128..2130,2131..2133,2134..2844)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2173..2194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2434..2453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2781..2802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5478..5599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5515..5534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5569..5588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5606..6061)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5743..5764)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5955..5974
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6088..6192
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6194..6551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6194..6385
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6217..6237)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6402..6537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6586..7380
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6586..7380
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6640..6659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7250..7269
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7568..7587)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7612..7659
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7988..8576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8477..8496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgaag atggacaaaa agactatagt ttggtttaga
      661 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
      721 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
      781 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
      841 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
      901 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
      961 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
     1021 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
     1081 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
     1141 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
     1201 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
     1261 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
     1321 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
     1381 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
     1441 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
     1501 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
     1561 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
     1621 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
     1681 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
     1741 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
     1801 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
     1861 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
     1921 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
     1981 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
     2041 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
     2101 atcatgatcg gagcagcagc ccgggtcatg gtgagcaagg gcgaggagct gttcaccggg
     2161 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2221 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2281 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     2341 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2401 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2461 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2521 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2581 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2641 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2701 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     2761 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2821 ctcggcatgg acgagctgta caagagcggc cgcatggcgg aaccttccca ggccccgacc
     2881 ccggccccgg ctgcgcagcc ccggcccctt cagtccccag cccctgcccc aactccgact
     2941 cctgcaccca gcccggcttc agccccgatt ccgactccca ccccggcacc agcccctgcc
     3001 ccagctgcag ccccagccgg cagcacaggg actggggggc ccggggtagg aagtgggggg
     3061 gccgggagcg ggggggatcc ggctcgacct ggcctgagcc agcagcagcg cgccagtcag
     3121 aggaaggcgc aagtccgggg gctgccgcgc gccaagaagc ttgagaagct aggggtcttc
     3181 tcggcttgca aggccaatga aacctgtaag tgtaatggct ggaaaaaccc caagcccccc
     3241 actgcacccc gcatggatct gcagcagcca gctgccaacc tgagtgagct gtgccgcagt
     3301 tgtgagcacc ccttggctga ccacgtatcc cacttggaga atgtgtcaga ggatgagata
     3361 aaccgactgc tggggatggt ggtggatgtg gagaatctct tcatgtctgt tcacaaggga
     3421 gaggacacag acaccaatca ggtctatttc tacctcttca agctactgcg gaaatgcatc
     3481 ctgcagatga cccggcctgt ggtggagggg tccctgggca gccctccatt tgagaaacct
     3541 aatattgagc agggtgtgct gaactttgtg cagtacaaat ttagtcacct ggctccccgg
     3601 gagcggcaga cgatgttcga gctctcaaag atgttcttgc tctgccttaa ctactggaag
     3661 cttgagacac ctgcccagtt tcggcagagg tctcaggctg aggacgtggc tacctacaag
     3721 gtcaattaca ccagatggct ctgttactgc cacgtgcccc agagctgtga tagcctcccc
     3781 cgctacgaaa ccactcatgt ctttgggcga agccttctcc ggtccatttt caccgttacc
     3841 cgccggcagc tgctggaaaa gttccgagtg gagaaggaca aattggtgcc cgagaagagg
     3901 accctcatcc tcactcactt ccccaaattc ctgtccatgc tggaggagga gatctatggg
     3961 gcaaactctc caatctggga gtcaggcttc accatgccac cctcagaggg gacacagctg
     4021 gttccccggc cagcttcagt cagtgcagcg gttgttccca gcacccccat cttcagcccc
     4081 agcatgggtg ggggcagcaa cagctccctg agtctggatt ctgcaggggc cgagcctatg
     4141 ccaggcgaga agaggacgct cccagagaac ctgaccctgg aggatgccaa gcggctccgt
     4201 gtgatgggtg acatccccat ggagctggtc aatgaggtca tgctgaccat cactgaccct
     4261 gctgccatgc tggggcctga gacgagcctg ctttcggcca atgcggcccg ggatgagaca
     4321 gcccgcctgg aggagcgccg cggcatcatc gagttccatg tcatcggcaa ctcactgacg
     4381 cccaaggcca accggcgggt gttgctgtgg ctcgtggggc tgcagaatgt cttttcccac
     4441 cagctgccgc gcatgcctaa ggagtatatc gcccgcctcg tctttgaccc gaagcacaag
     4501 actctggcct tgatcaagga tgggcgggtc atcggtggca tctgcttccg catgtttccc
     4561 acccagggct tcacggagat tgtcttctgt gctgtcacct cgaatgagca ggtcaagggt
     4621 tatgggaccc acctgatgaa ccacctgaag gagtatcaca tcaagcacaa cattctctac
     4681 ttcctcacct acgccgacga gtacgccatc ggcgcagcaa aaaagcaggg tttctccaag
     4741 gacatcaagg tgcccaagag ccgctacctg ggctacatca aggactacga gggagcgacg
     4801 ctgatggagt gtgagctgaa tccccgcatc ccctacacgg agctgtccca catcatcaag
     4861 aagcagaaag agatcatcaa gaagctgatt gagcgcaaac aggcccagat ccgcaaggtc
     4921 tacccggggc tcagctgctt caaggagggc gtgaggcaga tccctgtgga gagcgttcct
     4981 ggcattcgag agacaggctg gaagccattg gggaaggaga aggggaagga gctgaaggac
     5041 cccgaccagc tctacacaac cctcaaaaac ctgctggccc aaatcaagtc tcaccccagt
     5101 gcctggccct tcatggagcc tgtgaagaag tcggaggccc ctgactacta cgaggtcatc
     5161 cgcttcccca ttgacctgaa gaccatgact gagcggctgc gaagccgcta ctacgtgacc
     5221 cggaagctct ttgtggccga cctgcagcgg gtcatcgcca actgtcgcga gtacaacccc
     5281 ccggacagcg agtactgccg ctgtgccagc gccctggaga agttcttcta cttcaagctc
     5341 aaggagggag gcctcattga caagtagtct agatcataat cagccatacc acatttgtag
     5401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5461 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5581 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5641 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5701 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5761 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5821 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5881 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5941 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     6001 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     6061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6181 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     6241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6541 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     7021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     7081 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     7141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7381 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7441 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7501 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7561 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7621 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7681 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7741 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7801 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8101 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     8161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
