LOCUS       sequence-196758-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Live cell reader for MS2 loop RNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pmKate2-MS2coatProtein
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
       61 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      121 accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct
      181 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
      241 tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata cgaagacggg
      301 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      361 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
      421 tgggaggcct ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac
      481 atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga
      541 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg
      601 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
      661 agatactgcg acctccctag caaactgggg cacaga
//
