LOCUS       sequence-196762-        3180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription activator (transactivation domain of viral VP16 
            protein) that is constitutively recruited tetO arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: ptagBFP-TetR-NLS-VP16
FEATURES             Location/Qualifiers
     source          1..3180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(452..472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        457..786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      637..772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             831..1529
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1536..2159
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1612..1631)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             2181..2201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2584..2705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2621..2640)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2675..2694
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2712..3166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2849..2870)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3060..3079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
       61 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      421 cgtattaccg ccatgcatta gttattactg tggaatgtgt gtcagttagg gtgtggaaag
      481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      721 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      781 tgcaaaaagc tagcgctacc ggggccgctc tagcgctacc ggtcgccacc atgagcgagc
      841 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact
      901 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
      961 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct
     1021 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct
     1081 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     1141 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     1201 tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca
     1261 ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc
     1321 tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc aagaaacccg
     1381 ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg
     1441 aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc
     1501 tccctagcaa actggggcac aagcttaatt ccggaatggt gtctagatta gataaaagta
     1561 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     1621 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     1681 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     1741 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     1801 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     1861 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     1921 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     1981 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     2041 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     2101 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctg
     2161 accgtcgact gggtacccct cctaagaaga agaggaaggt tgcccccacc atggataaaa
     2221 agaagagaaa ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg
     2281 aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg
     2341 acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg
     2401 atatggccga cttcgagttt gagcagatgt ttaccgatgc ccctctagac ggggggatcc
     2461 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     2521 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2581 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2641 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2701 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2761 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2821 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2881 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2941 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aagcactaaa tcggaaccct
     3001 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3061 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3121 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
//
