LOCUS       sequence-196773-        2033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flourescent marker for lacO arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Rademacher A, Erdel F, Trojanowski J, Schumacher S, Rippe K
  TITLE     Real-time observation of light-controlled transcription in living 
            cells.
  JOURNAL   J Cell Sci. 2017 Dec 15;130(24):4213-4224. doi: 10.1242/jcs.205534. 
            Epub 2017 Nov 9.
  PUBMED    29122982
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.205534"; journalName: "J Cell Sci"; date: "2017-12-15- 
            15"; volume: "130"; issue: "24"; pages: "4213-4224"
COMMENT     Sequence Label: pCFP-LacI-NLS
FEATURES             Location/Qualifiers
     source          1..2033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..200
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      50..186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      219..228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(225..227,228..230,231..941)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(270..291)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(531..550)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     878..899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(979..998)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 catgcatctc aattagtcag caaccatagt cccgccctta actccgccca tcccgcccct
       61 aactccgccc agttccgccc attctccgcc cccatggctg actaattttt tttatttatg
      121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
      181 gaggcctagg cttttgcaaa aagctagcgc taccggtcgc caccatggtg agcaagggcg
      241 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      301 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      361 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      421 cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      481 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      541 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      601 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      661 acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
      721 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      781 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      841 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      901 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatccatgg
      961 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     1021 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     1081 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     1141 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     1201 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     1261 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     1321 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     1381 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     1441 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     1501 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     1561 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     1621 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     1681 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     1741 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     1801 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     1861 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     1921 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     1981 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgaagcagc ctg
//
