LOCUS       sequence-196864-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression plasmid with gRNA 
            targetting ADE2 from Ogataea (para)polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP013
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            61..1638
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             409..1437
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             409..1437
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             2016..2039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2043..2063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2064..6164
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6168..6188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        7107..7773
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7643..7666
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           7780..7816
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        7837..7912
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           7917..7984
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(8026..8044)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8478..9269,9270..9338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9101..9120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9339..9443)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9546..10134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10035..10054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac
       61 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
      121 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      181 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
      241 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      301 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      361 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gggtaaaaag
      421 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc
      481 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
      541 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
      601 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt
      661 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
      721 cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct
      781 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
      841 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
      901 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
      961 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     1021 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     1081 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     1141 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     1201 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     1261 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     1321 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     1381 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataactc
     1441 gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     1501 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     1561 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     1621 atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga cgatgcccgt
     1681 gtctaaatga ttcgaccagc ctaagaatgt tcaacaatct ataatcagtc catagtcaac
     1741 aagagccctc aaagttgata tcgtttagtg atctccctcg agatgagcgg aggatggttg
     1801 ctttttgggg agccctaagt ggtacgtgcg gcgagcggga tgtttcccca ttaggcaaag
     1861 gacgaccggg taacccctcg agaaaaaaaa tttttcgccg ctaatctggt gttatataaa
     1921 gctccccctg ctcctggaat ttttttcctt gtcaactcac accggaaatc aaggcatttc
     1981 attctgagta gttcttaaaa acataatcaa caatggatta taaagatgac gatgacaaac
     2041 ctccaaaaaa gaagagaaag gtcgataaga aatactcaat aggcttagat atcggcacaa
     2101 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
     2161 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
     2221 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
     2281 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
     2341 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
     2401 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
     2461 ctatctatca tctgcgaaaa aaattggtag attctactta taaagcggat ttgcgcttaa
     2521 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt
     2581 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc
     2641 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg
     2701 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga
     2761 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat
     2821 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg
     2881 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta
     2941 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta
     3001 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc
     3061 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc
     3121 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata
     3181 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa
     3241 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt acccatcaaa
     3301 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa
     3361 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc
     3421 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta
     3481 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac
     3541 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc
     3601 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa
     3661 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca
     3721 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat
     3781 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct
     3841 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag
     3901 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg
     3961 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc
     4021 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc
     4081 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta
     4141 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt
     4201 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta
     4261 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc
     4321 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc
     4381 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc
     4441 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct
     4501 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa
     4561 gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca
     4621 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag
     4681 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc
     4741 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag
     4801 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa
     4861 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta
     4921 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata
     4981 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg
     5041 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata
     5101 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg
     5161 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa
     5221 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct
     5281 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata
     5341 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa
     5401 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt
     5461 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat
     5521 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct
     5581 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact
     5641 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc
     5701 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga
     5761 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac
     5821 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca
     5881 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat
     5941 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta
     6001 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta
     6061 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg
     6121 gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccctcca aaaaagaaga
     6181 gaaaggtctg agcggttttg tataactaaa taatattgga aactaaatac gaatacccaa
     6241 attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga aacaggtaaa
     6301 tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt attagtcgcc
     6361 gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg tataagggaa
     6421 actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg aaactacggc aaaggattgg
     6481 tcagatcgct tcatacaggg aaagttcggc atcaacatct ttggataata tcagaatgag
     6541 aaagaacaga tacgcagtac gttttttggt gagctctttg cacttcttta gttctttcca
     6601 tcaatatcag ttttttaaac ttttaggact aaaagtgatg tttaacttca aaatgtttaa
     6661 aattttgttc ttcccgacgt tcattaagaa tactaataca ctttaataat tattttaata
     6721 ttttgttcta aataatgact tttaattaaa aaagataaaa tataaaaaca tcataataac
     6781 tcaccagagg ttaagaacaa aaaaacaaat tagatatctg ctaatccaat atagttaaat
     6841 caatctttcc ttggtataat gggtatatta catatatttc aaggaccgac actcctacca
     6901 aatatctaaa atttaccata ttaacataac atgtatataa acgtcaaatc ataatcagca
     6961 ctaacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc gaggacacgc
     7021 tagggccaag gcaaaaagat tccttgatta cgtaagggag ttagaatcat tttgaataaa
     7081 aaacacgctt tttcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta
     7141 aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc
     7201 tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta
     7261 ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta
     7321 agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca
     7381 tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa
     7441 aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc
     7501 aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct
     7561 ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac
     7621 ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt
     7681 aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac
     7741 ttagtttcga ataaacacac ataaacaaac aaattccagc tgatgagtcc gtgaggacga
     7801 aacgagtaag ctcgtcctgg aattgatctg cttggcgttt tagagctaga aatagcaagt
     7861 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttggcc
     7921 ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg
     7981 ggacacaggc cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc
     8041 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     8101 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
     8161 ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct
     8221 tgagaaggtt ttgggacaag cttacgtctc acggatcgta tatgccgtag cgacaatcta
     8281 agaactatgc gaggacacgc tagcagctgt attcacgtag acggataggt atagccagac
     8341 atcagcagca tacttcggga accgtaggcc accgagcggc cgcgattatc aaaaaggatc
     8401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8581 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
     8641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa
     9481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10201 cctgcgttat cccctgattc tgtggataac cgtgcgg
//
