LOCUS       sequence-19687-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Mizuuchi K, Mizuuchi M, O'Dea MH, Gellert M
  TITLE     Cloning and simplified purification of Escherichia coli DNA gyrase A
            and B proteins.
  JOURNAL   J Biol Chem. 1984 Jul 25. 259(14):9199-201.
  PUBMED    6086626
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1984 Jul 25. 259(14):9199-201."
COMMENT     Sequence Label: pMK90
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(129..233)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     301..319
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
       61 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      241 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
      301 aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac agcttatcat
      361 cgataagctt cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac
      421 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
      481 atctggacaa gggaaaacgc aagcgcanag agaaagcagg tagcttgcag tgggcttaca
      541 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
      601 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgccn
      661 aggatctgat ggcgcagggg atcaagatcc cgtgatgacc tcattaaaaa cacgctgcaa
      721 tccctcctca tctttgcagg caagtccgat ttttttg
//
