LOCUS       sequence-196872-        6117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Associated Kinase, Excitation: 554, Emission:
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6117)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-FYN-N-10
FEATURES             Location/Qualifiers
     source          1..6117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2013..2022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2019..2720
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2152..2170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2420..2439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2614..2637
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2845..2966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2882..2901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2936..2955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2973..3428)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3110..3131)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3322..3341
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3455..3559
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3561..3918
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3561..3752
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3584..3604)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3769..3904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3953..4747
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3953..4747
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4007..4026)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4617..4636
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4935..4954)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4979..5026
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5355..5943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5844..5863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgggctg tgtgcaatgt aaggataaag aagcaacaaa actgacggag gagagggacg
      601 gcagcctgaa ccagagctct gggtaccgct atggcacaga ccccacccct cagcactacc
      661 ccagcttcgg tgtgacctcc atccccaact acaacaactt ccacgcagcc gggggccaag
      721 gactcaccgt ctttggaggt gtgaactctt cgtctcatac ggggaccttg cgtacgagag
      781 gaggaacagg agtgacactc tttgtggccc tttatgacta tgaagcacgg acagaagatg
      841 acctgagttt tcacaaagga gaaaaatttc aaatattgaa cagctcggaa ggagattggt
      901 gggaagcccg ctccttgaca actggagaga caggttacat tcccagcaat tatgtggctc
      961 cagttgactc tatccaggca gaagagtggt actttggaaa acttggccga aaagatgctg
     1021 agcgacagct attgtccttt ggaaacccaa gaggtacctt tcttatccgc gagagtgaaa
     1081 ccaccaaagg tgcctattca ctttctatcc gtgattggga tgatatgaaa ggagaccatg
     1141 tcaaacatta taaaattcgc aaacttgaca atggtggata ctacattacc acccgggccc
     1201 agtttgaaac acttcagcag cttgtacaac attactcagg tacctggaat ggaaacacaa
     1261 aagtagccat aaagactctt aaaccaggca caatgtcccc cgaatcattc cttgaggaag
     1321 cgcagatcat gaagaagctg aagcacgaca agctggtcca gctctatgca gtggtgtctg
     1381 aggagcccat ctacatcgtc accgagtata tgaacaaagg aagtttactg gatttcttaa
     1441 aagatggaga aggaagagct ctgaaattac caaatcttgt ggacatggca gcacaggtgg
     1501 ctgcaggaat ggcttacatc gagcgcatga attatatcca tagagatctg cgatcagcaa
     1561 acattctagt ggggaatgga ctcatatgca agattgctga cttcggattg gcccgattga
     1621 tagaagacaa tgagtacaca gcaagacaag gtgcaaagtt ccccatcaag tggacggccc
     1681 ccgaggcagc cctgtacggg aggttcacaa tcaagtctga cgtgtggtct tttggaatct
     1741 tactcacaga gctggtcacc aaaggaagag tgccataccc aggcatgaac aaccgggagg
     1801 tgctggagca ggtggagcga ggctacagga tgccctgccc gcaggactgc cccatctctc
     1861 tgcatgagct catgatccac tgctggaaaa aggaccctga agaacgcccc acttttgagt
     1921 acttgcagag cttcctggaa gactacttta ccgcgacaga gccccagtac caacctggtg
     1981 aaaacctggc atccagcggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
     2041 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     2101 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     2161 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     2221 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     2281 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     2341 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     2401 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     2461 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     2521 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     2581 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     2641 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     2701 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     2761 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2821 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2941 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3001 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3061 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3121 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3181 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3241 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3301 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3361 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3421 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3541 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3601 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3661 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3721 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3781 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3841 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3901 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3961 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4021 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4081 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4141 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4201 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4261 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4321 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4381 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4441 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4501 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4561 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4621 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4681 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4741 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4921 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4981 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5041 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5101 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5161 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5221 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5281 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5341 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5401 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5461 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5521 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5581 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5641 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5701 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5761 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5821 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5881 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5941 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6001 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag
     6061 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
