LOCUS       sequence-196873-        3912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full-length human b2 with tension sensor at b2_743.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Nordenfelt P, Elliott HL, Springer TA
  TITLE     Coordinated integrin activation by actin-dependent force during 
            T-cell migration.
  JOURNAL   Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.
  PUBMED    27721490
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-10"; volume: "7:13119. doi"; pages: " 
            10.1038/ncomms13119"
COMMENT     Sequence Label: b2-TS5
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2254..2961
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3082..3084,3085..3087,3088..3798)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3127..3148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3388..3407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3735..3756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcca ccatgctggg cctgcgcccc ccactgctcg ccctggtggg gctgctctcc
       61 ctcgggtgcg tcctctctca ggagtgcacg aagttcaagg tcagcagctg ccgggaatgc
      121 atcgagtcgg ggcccggctg cacctggtgc cagaagctga acttcacagg gccgggggat
      181 cctgactcca ttcgctgcga cacccggcca cagctgctca tgaggggctg tgcggctgac
      241 gacatcatgg accccacaag cctcgctgaa acccaggaag accacaatgg gggccagaag
      301 cagctgtccc cacaaaaagt gacgctttac ctgcgaccag gccaggcagc agcgttcaac
      361 gtgaccttcc ggcgggccaa gggctacccc atcgacctgt actatctgat ggacctctcc
      421 tactccatgc ttgatgacct caggaatgtc aagaagctag gtggcgacct gctccgggcc
      481 ctcaacgaga tcaccgagtc cggccgcatt ggcttcgggt ccttcgtgga caagaccgtg
      541 ctgccgttcg tgaacacgca ccctgataag ctgcgaaacc catgccccaa caaggagaaa
      601 gagtgccagc ccccgtttgc cttcaggcac gtgctgaagc tgaccaacaa ctccaaccag
      661 tttcagaccg aggtcgggaa gcagctgatt tccggaaacc tggatgcacc cgagggtggg
      721 ctggacgcca tgatgcaggt cgccgcctgc ccggaggaaa tcggctggcg caacgtcacg
      781 cggctgctgg tgtttgccac tgatgacggc ttccatttcg cgggcgacgg aaagctgggc
      841 gccatcctga cccccaacga cggccgctgt cacctggagg acaacttgta caagaggagc
      901 aacgaattcg actacccatc ggtgggccag ctggcgcaca agctggctga aaacaacatc
      961 cagcccatct tcgcggtgac cagtaggatg gtgaagacct acgagaaact caccgagatc
     1021 atccccaagt cagccgtggg ggagctgtct gaggactcca gcaatgtggt ccatctcatt
     1081 aagaatgctt acaataaact ctcctccagg gtcttcctgg atcacaacgc cctccccgac
     1141 accctgaaag tcacctacga ctccttctgc agcaatggag tgacgcacag gaaccagccc
     1201 agaggtgact gtgatggcgt gcagatcaat gtcccgatca ccttccaggt gaaggtcacg
     1261 gccacagagt gcatccagga gcagtcgttt gtcatccggg cgctgggctt cacggacata
     1321 gtgaccgtgc aggttcttcc ccagtgtgag tgccggtgcc gggaccagag cagagaccgc
     1381 agcctctgcc atggcaaggg cttcttggag tgcggcatct gcaggtgtga cactggctac
     1441 attgggaaaa actgtgagtg ccagacacag ggccggagca gccaggagct ggaaggaagc
     1501 tgccggaagg acaacaactc catcatctgc tcagggctgg gggactgtgt ctgcgggcag
     1561 tgcctgtgcc acaccagcga cgtccccggc aagctgatat acgggcagta ctgcgagtgt
     1621 gacaccatca actgtgagcg ctacaacggc caggtctgcg gcggcccggg gagggggctc
     1681 tgcttctgcg ggaagtgccg ctgccacccg ggctttgagg gctcagcgtg ccagtgcgag
     1741 aggaccactg agggctgcct gaacccgcgg cgtgttgagt gtagtggtcg tggccggtgc
     1801 cgctgcaacg tatgcgagtg ccattcaggc taccagctgc ctctgtgcca ggagtgcccc
     1861 ggctgcccct caccctgtgg caagtacatc tcctgcgccg agtgcctgaa gttcgaaaag
     1921 ggcccctttg ggaagaactg cagcgcggcg tgtccgggcc tgcagctgtc gaacaacccc
     1981 gtgaagggca ggacctgcaa ggagagggac tcagagggct gctgggtggc ctacacgctg
     2041 gagcagcagg acgggatgga ccgctacctc atctatgtgg atgagagccg agagtgtgtg
     2101 gcaggcccca acatcgccgc catcgtcggg ggcaccgtgg caggcatcgt gctgatcggc
     2161 attctcctgc tggtcatctg gaaggctctg atccacctga gcgacctccg ggagtacagg
     2221 cgctttgaga aggagaagct ccaggctcag gcgatggtga gcaagggcga ggagaccaca
     2281 atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc
     2341 cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc
     2401 aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg
     2461 ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag
     2521 cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc
     2581 gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc
     2641 aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac
     2701 gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag
     2761 ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag
     2821 aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac
     2881 gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac
     2941 ggcatggacg agctgtacaa ggggccaggt ggtgcagggc caggtggtgc agggccaggt
     3001 ggtgcagggc caggtggtgc agggcccggt ggtgcaggtc caggtggtgc aggtccaggt
     3061 ggtgcaggtc caggtggtgc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     3121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3181 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     3241 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     3301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3541 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     3601 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3661 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     3721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3781 atggacgagc tgtacaaggc tcaagcacaa aaggagaagc tcaagtccca gtggaacaat
     3841 gataatcccc ttttcaagag cgccaccacg acggtcatga accccaagtt tgctgagagt
     3901 tagtaaaagc tt
//
