LOCUS       sequence-196878-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-host-range Cas9/gRNA co-expression plasmid with gRNA 
            targetting ADE2 from Kluyveromyces marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, 
            Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
  TITLE     Genome editing in Kluyveromyces and Ogataea yeasts using a 
            broad-host-range Cas9/gRNA co-expression plasmid.
  JOURNAL   FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 
            10.1093/femsyr/foy012.
  PUBMED    29438517
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
            " 10.1093/femsyr/foy012"
COMMENT     Sequence Label: pUDP082
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1029
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1..1029
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             1608..1631
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1635..1655
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1656..5756
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5760..5780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        6699..7365
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7235..7258
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     ncRNA           7372..7408
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        7429..7504
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           7509..7576
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(7618..7636)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8070..8861,8862..8930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8693..8712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8931..9035)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9138..9726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9627..9646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            join(9890..10237,1..1230)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
ORIGIN
        1 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
       61 gacagcgtgt ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      121 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      181 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
      241 attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
      301 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg
      361 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
      421 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
      481 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
      541 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
      601 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
      661 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
      721 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
      781 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
      841 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
      901 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
      961 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     1021 aaggaataac tcgaggacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1081 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1141 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1201 aatcgtatgt gaatgctggt cgctatactg gagctctcga gaaccctcac ctttcgagag
     1261 gacgatgccc gtgtctaaat gattcgacca gcctaagaat gttcaacaat ctataatcag
     1321 tccatagtca acaagagccc tcaaagttga tatcgtttag tgatctccct cgagatgagc
     1381 ggaggatggt tgctttttgg ggagccctaa gtggtacgtg cggcgagcgg gatgtttccc
     1441 cattaggcaa aggacgaccg ggtaacccct cgagaaaaaa aatttttcgc cgctaatctg
     1501 gtgttatata aagctccccc tgctcctgga atttttttcc ttgtcaactc acaccggaaa
     1561 tcaaggcatt tcattctgag tagttcttaa aaacataatc aacaatggat tataaagatg
     1621 acgatgacaa acctccaaaa aagaagagaa aggtcgataa gaaatactca ataggcttag
     1681 atatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta
     1741 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     1801 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta
     1861 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga
     1921 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     1981 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     2041 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact tataaagcgg
     2101 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     2161 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     2221 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     2281 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     2341 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     2401 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     2461 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     2521 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     2581 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     2641 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     2701 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     2761 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     2821 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     2881 ttacccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     2941 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt
     3001 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     3061 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     3121 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa
     3181 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     3241 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     3301 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     3361 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     3421 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     3481 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     3541 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     3601 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta
     3661 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     3721 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     3781 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     3841 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     3901 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     3961 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     4021 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     4081 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     4141 ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg
     4201 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     4261 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     4321 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     4381 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     4441 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     4501 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     4561 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     4621 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     4681 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     4741 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     4801 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     4861 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     4921 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa
     4981 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     5041 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     5101 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg
     5161 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     5221 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     5281 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     5341 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     5401 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     5461 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     5521 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     5581 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     5641 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     5701 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgaccctc
     5761 caaaaaagaa gagaaaggtc tgagcggttt tgtataacta aataatattg gaaactaaat
     5821 acgaataccc aaatttttta tctaaatttt gccgaaagat taaaatctgc agagatatcc
     5881 gaaacaggta aatggatgtt tcaatccctg tagtcagtca ggaacccata ttatattaca
     5941 gtattagtcg ccgcttaggc acgcctttaa ttagcaaaat caaaccttaa gtgcatatgc
     6001 cgtataaggg aaactcaaag aactggcatc gcaaaaatga aaacatacgt tgaaactacg
     6061 gcaaaggatt ggtcagatcg cttcatacag ggaaagttcg gcatcaacat ctttggataa
     6121 tatcagaatg agaaagaaca gatacgcagt acgttttttg gtgagctctt tgcacttctt
     6181 tagttctttc catcaatatc agttttttaa acttttagga ctaaaagtga tgtttaactt
     6241 caaaatgttt aaaattttgt tcttcccgac gttcattaag aatactaata cactttaata
     6301 attattttaa tattttgttc taaataatga cttttaatta aaaaagataa aatataaaaa
     6361 catcataata actcaccaga ggttaagaac aaaaaaacaa attagatatc tgctaatcca
     6421 atatagttaa atcaatcttt ccttggtata atgggtatat tacatatatt tcaaggaccg
     6481 acactcctac caaatatcta aaatttacca tattaacata acatgtatat aaacgtcaaa
     6541 tcataatcag cactaacgtc tcacggatcg tatatgccgt agcgacaatc taagaactat
     6601 gcgaggacac gctagggcca aggcaaaaag attccttgat tacgtaaggg agttagaatc
     6661 attttgaata aaaaacacgc tttttcagtt cgagtttatc attatcaata ctgccatttc
     6721 aaagaatacg taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta
     6781 gccttttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata
     6841 tataacatcg taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga
     6901 gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt
     6961 cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca
     7021 caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg
     7081 atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac
     7141 cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa
     7201 ttattcccct acttgactaa taagtatata aagacggtag gtattgattg taattctgta
     7261 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
     7321 aacaccaaga acttagtttc gaataaacac acataaacaa acaaagtcaa gctgatgagt
     7381 ccgtgaggac gaaacgagta agctcgtcgc ccatttttct gcgtataggt tttagagcta
     7441 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     7501 gtgcttttgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg
     7561 gcatggcgaa tgggacacag gccccttttc ctttgtcgat atcatgtaat tagttatgtc
     7621 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     7681 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     7741 tatatttcaa atttttcttt tttttctgta caaacgcgtg tacgcatgta acattatact
     7801 gaaaaccttg cttgagaagg ttttgggaca agcttacgtc tcacggatcg tatatgccgt
     7861 agcgacaatc taagaactat gcgaggacac gctagcagct gtattcacgt agacggatag
     7921 gtatagccag acatcagcag catacttcgg gaaccgtagg ccaccgagcg gccgcgatta
     7981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8041 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc
     8221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9061 gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9781 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtgcgga ctatatgtga
     9841 aggcatggct atggcacggc agacattccg ccagatcatc aataggcacg acatggaggc
     9901 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
     9961 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
    10021 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
    10081 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
    10141 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
    10201 cagttctcac atcacatccg aacataaaca aggatcc
//
