LOCUS       sequence-19690-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recombination substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Hesse JE, Lieber MR, Mizuuchi K, Gellert M
  TITLE     V(D)J recombination: a functional definition of the joining signals.
  JOURNAL   Genes Dev. 1989 Jul . 3(7):1053-61.
  PUBMED    2777075
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1989 Jul . 3(7):1053-61."
COMMENT     Sequence Label: pJH299
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(206..240)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(451..467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(451..467)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(464..486)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    475..491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(499..505,506..523,524..529))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    544..565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(682..699)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgccaa gctgatcccc ggggatcccg agggtttttg tacagccaga cagtggagta
      121 ctaccactgt gggatcctct catcgatgag aggatcgacg acgacatggc tcgattggcg
      181 cgacaagttg ctgcgattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      241 caaatccaga tggagttctg aggtcattac tggagacctg ccgagtggtt cagcaggtgg
      301 aagagggact ggattccaaa gttctcaatg ctgcttgctg ttcttgaatg gggggtcgtt
      361 gatcccccat cgatgagagt cgactgcagg gtttttgttc cagtctgtag cactgtggtc
      421 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      481 tccgctcaca attccaacca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      541 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      601 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggnttgcg
      661 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      721 gcgagcggta tcagc
//
