LOCUS       sequence-196906-        2938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PTHI11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pTHI11
FEATURES             Location/Qualifiers
     source          1..2938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1234..1250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1234..1250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1298..1329
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1330..1432
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1433..2248
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1506..1525)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2272..2299
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2311..2898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2391..2410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2938,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagatc ttttcagctt catcgtcagt gatatttctc
      181 agcccacaga ccaagtcaac tttggaatct aacaaccttg ttcttacaat gttagaactc
      241 ttaagtcgca tgccatgatc ttcaagctga attttgtgaa ggaggtcaaa ccccacaatg
      301 gcatctagtt gtttagaata catgccttcg acaagtgttt gagtgtccaa aatcaagagc
      361 tcaaaattat tgaatttgtc tgccaataac gccgtaaatt gattagtgtc cagcccacca
      421 acaataggag cacctatagt taatttttca gataaattta agttatcaag gtaaaggagc
      481 tctaagttta ccccttccaa cagggttatt tgagaactca ataaattgtt gaattcaaaa
      541 ccaattgtct ttgaattctc cactggagct tccttgctga aattgatttt gataccattg
      601 gcatcgaata gacccgtatg ataactccat aaaaagggga gatgataggc cttaaattca
      661 tcgttaatct gcaaatttat tcctgacatg tctttgtaaa tagttatagt tcagaaactg
      721 gaattgagct caaaaaactg gaatcgagcg gatatttgaa gattgatgcc ttactcatga
      781 attgattgat aagagctccg tgattcactc tgtcaatgat tacccctctc ctacccgatt
      841 tgggactttt tcttcagtct tggggacttt ttttcatatg acttgacctt gctttcccaa
      901 tagggaagga ctcacccatg gatgattaag tttggattac tcgtttagga aatagtagcc
      961 atgaatcaat ttgaatcata ccatcatgaa atagggttag gctgtaaatg cctcaaaaat
     1021 ggctcttgag gctggatttt tgggtattgg aatgttggta gcaattggta taaaaggcca
     1081 tttgtatttc acttttttgt ccttcatact ttactcttct caactttgga aacttcaata
     1141 aatcatccat ggcgtcttcg aattcgcgcg tgatcttacg gcattatacg tatgatcggt
     1201 ccacgatcag ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata
     1261 ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg
     1321 cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1381 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca
     1441 tattcaacgg gaaacgtctt gcttgaagcc gcgattaaat tccaacatgg atgctgattt
     1501 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     1561 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1621 tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac
     1681 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg
     1741 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1801 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg
     1861 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc
     1921 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1981 taaactcttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2041 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2101 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2161 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2221 tcacttgatg ctcgatgagt ttttctaatg aggacctaaa tgtaatcacc tggctcacct
     2281 tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2341 tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2761 tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc
     2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2881 agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgt
//
