LOCUS       sequence-196910-        1940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector L-R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1920 HC_Kan_conn_L-R
FEATURES             Location/Qualifiers
     source          1..1940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      285..316
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        317..419
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             420..1235
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(493..512)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1256..1283
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1295..1882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1375..1394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1904..1933)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttgcat ccacgatcta gtcaccgtct cacgtctatg atgaggatgt tggtggagag
      121 catgtggagg aagtggatag ggaaggttgt agagtagatc cggttgaagt gatgaggata
      181 ggaggaggcg gtcgagacgc tactcaccct cgagcataac gacgaattct ctagatatcg
      241 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga
      301 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
      361 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      421 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      481 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      541 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      601 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc
      661 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      721 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      781 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      841 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      901 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      961 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1021 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     1081 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     1141 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     1201 tgcagtttca cttgatgctc gatgagtttt tctgagggcc caaatgtaat cacctggctc
     1261 accttcgggt gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1321 agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1741 tatttggtat ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg
     1801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1861 gcgcagaaaa aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc
     1921 gtgaaggtga gccagtgagt
//
