LOCUS       sequence-196912-        1927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector for second step assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe4522 HC_Kan_conn_BsaI-3_p1
FEATURES             Location/Qualifiers
     source          1..1927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      207..238
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        239..341
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             342..1157
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(415..434)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1178..1205
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1217..1804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1297..1316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1826..1855)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ctagtcaccg tctcgtagga gagcatggag aatgggagag aaagaaggga tgaaacaacg
       61 caacgaacga aaaggtctca atcccagctg ttaactagcg agtcagacga gatggataga
      121 tttagatgca tggcgagacg ctaacgaatt ctctagatat cgctcaatac tgaccattta
      181 aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg
      241 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      301 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg
      361 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
      421 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
      481 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
      541 atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
      601 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat
      661 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
      721 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat
      781 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg
      841 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
      901 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
      961 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1021 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1081 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc
     1141 tcgatgagtt tttctgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt
     1201 ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat
     1261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1621 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1681 tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1741 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1801 tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgaaggt gagccagtga
     1861 gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata tcaagcttga
     1921 attcgtt
//
