LOCUS       sequence-196916-        7379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  deletes synpcc7942_1377 (spoT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     ppGpp Controls Global Gene Expression in Light and in Darkness in S.
            elongatus.
  JOURNAL   Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 
            10.1016/j.celrep.2017.11.067.
  PUBMED    29241543
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.11"; journalName: "Cell Rep"; date: 
            "2017-12-12- 12"; volume: "21"; issue: "11"; pages: "3155-3165"
COMMENT     Sequence Label: spoT-
FEATURES             Location/Qualifiers
     source          1..7379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1063..1878)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNIDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEKVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1786..1805
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1870..1889
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3104..4294
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3456..3475)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4933..5124
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5118..5140)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5226..5366
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5281..5300
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5381..5398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5552..6140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5632..5651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6311..7102,7103..7171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6934..6953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7172..7276)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7344..7362
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgatat ggatggcggc ataactggcg ctagacatcg
       61 gggctaatcg acagtgtcaa accaaaacgg ttagccagcc ctgaacagcg atcgcttcca
      121 gccagacagg gcagccgttt ccacttctag gcaggtcaac gtccagactg ccgttgattc
      181 gagcttaacg ttcccttacc aagcactggt cagcaagact tccagcactt gattgggttg
      241 aagactcgtc gccggcaagc cactcccaaa ggctgatgca gctccgacag cagccccccc
      301 gactaacccc acgggtcctc caaccactag tcctgccgct gcgcctacac ctgcattgtt
      361 ggcaacacga ccgccatcag ccgtaggcgg gccggtgatg acctctgaaa tggcatagat
      421 cggtactgag actccattga ggacaagcgc ttgtgcaaca aaccgactgc cctgctgatt
      481 agtttcaaag cggccgagca ctggcgtccc gatcggtagg atgacctggc ccctgctatc
      541 cgtgacattt tggtcaacag tcatgacctc ttgaattggt agagcgcttt gcaagctcag
      601 accaggacct gtgtagcgga gccgcaccac aaaccgatcg ctcaagggtt tcaagggggt
      661 tgtgctgagt gacgagccac cgccagccgg cacggtcggc agtggaatac tggactgggc
      721 ccaagcggtc gaagctgaca gtgcggctaa cacgccagcg atcgcgactg cagatttgcg
      781 catcatttcc atccatcgcc tttcgcacag ctagcttgcc attttcccac caggaatcaa
      841 gacctaggtt tgccgtttgc accaagaatg actggattca gacccagaat cttgcgattc
      901 agtccactag cattcctgtc acccccctga ctcacaggaa attaacggtt aagatcggtg
      961 cacaactgcc gagcttccca atgaactgct gcagttgcac cagccaaaag gggagcgatc
     1021 gcacgttcgc tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
     1081 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     1141 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     1201 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     1261 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     1321 caaaagctta tgcatttctt tccagacttt ttcaacaggc cagccattac gctcgtcatc
     1381 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     1441 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     1501 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     1561 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     1621 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     1681 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     1741 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     1801 atacccatat aaatcagcat caatgttgga atttaatcgc ggcctcgagc aagacgtttc
     1861 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     1921 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac
     1981 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc
     2041 cgacaacgca gtcactcagc tatccaactg atgctgataa ataccgccta caggcactta
     2101 ttgcgctgca ggagttagca attgagccgc gatcgccgct agtttttggg ccgcaccggc
     2161 ctcaccgcga accgctctga gttccgcttg catggccgac agtttctctg ggttctggag
     2221 atagtcgaga gcgatcgcgg caatatcctc gggatagtat tcgccgatca actcgggcac
     2281 gatcgcccgc ttcgcccaaa tattcggcca agccagcaga cccactttgc gcagcgccaa
     2341 ggtattaatc aggctagcga tcgcattgcc caacagtggc accttgacca gcaagcccgg
     2401 caagccgtcc caggctttca tcgcatcccg tttttgggtt ggcagcagca ccaacatcgg
     2461 caccgccagc gcgcctaatt ctgctgtatt cgcgccgatc gtggtcaggc agatctggca
     2521 ctgggccaac tcgacatagg ctggaaattc cgtgatcaac cttacggtta gccctttttc
     2581 ggtcgtcagc acccaacctg tgggttgctc ttctaaacgg gcggaactac cccgaaacat
     2641 tgcaatgacg ggattttgat ccgcatccgc atagcgagcg atctgctgcg gttgcacggt
     2701 cggtgcgagt ggcagaataa actccatctc tggttgctga gccgcgatcg cttcagccgc
     2761 cgccagcatg aagggcaagc cgatctgcaa tttagctgct ttggatccgg gcagaagacc
     2821 cacccgcgat cgtgtggatt cagatccagc ctgcgctgag acctccgcca ttaaatcgcc
     2881 aaccaactga aacttgtgac ggtctttctg cggtgctttc tcaatcacct ggggcgtcat
     2941 taccgcaaag gcatcagccc agccagtcca gcgggcttcc cattccgcgt agatcaagca
     3001 gcggtagccc aagcgtcggg caatccagag ggcaaaaaac tgatcgccgc cttaatgcgg
     3061 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
     3121 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
     3181 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     3241 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     3301 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     3361 ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac gcatcgtggc
     3421 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     3481 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     3541 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     3601 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     3661 gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3721 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3781 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     3841 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     3901 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     3961 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     4021 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     4081 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     4141 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     4201 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     4261 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
     4321 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     4381 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
     4441 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
     4501 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
     4561 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
     4621 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
     4681 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     4741 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     4801 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     4861 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     4921 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     4981 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     5041 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     5101 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5161 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5221 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     5281 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5341 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     5401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6601 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     6661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6901 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     6961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7321 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
