LOCUS       sequence-196925-       10229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses huLbCpf1-T2A-GFP and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY095
FEATURES             Location/Qualifiers
     source          1..10229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..72)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        57..386
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      237..372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     299..318
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             453..1247
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(507..526)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1117..1136
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1421..1542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1458..1477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1512..1531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1591..1607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1591..1607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1604..1626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1615..1631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1639..1645,1646..1663,1664..1669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1684..1705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2752..3543,3544..3612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3375..3394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3613..3717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3792..3811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3918..4166
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3918..3938
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4089..4108
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4287..4666
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4667..4870
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4821..4841
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4915..4934
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4915..4933
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4981..8664
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             8665..8712
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8728..8781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8782..8784,8785..9498)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8782..8784,8785..9495)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8824..8845)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9085..9104)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9432..9453
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9568..9585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9574..9798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9844..10229,1..43)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9931..9950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10141..10162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
       61 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      121 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
      181 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
      241 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      301 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
      361 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
      421 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
      481 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
      541 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
      601 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
      661 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
      721 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
      781 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
      841 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
      901 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
      961 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     1021 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     1081 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     1141 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     1201 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     1261 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     1321 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     1381 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     1441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     1561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3721 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     3781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     3841 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     3901 ttgaccgact taattaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     3961 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     4021 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     4081 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     4141 atatcttgtg gaaaggacga aacaccgtaa tttctactaa gtgtagatgg agacgatata
     4201 tcgtctcttt ttcaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     4261 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     4321 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4381 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4441 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4501 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4561 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4621 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4681 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4741 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4801 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4861 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
     4921 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgccacc
     4981 atgagcaagc tggagaagtt tacaaactgc tactccctgt ctaagaccct gaggttcaag
     5041 gccatccctg tgggcaagac ccaggagaac atcgacaata agcggctgct ggtggaggac
     5101 gagaagagag ccgaggatta taagggcgtg aagaagctgc tggatcgcta ctatctgtct
     5161 tttatcaacg acgtgctgca cagcatcaag ctgaagaatc tgaacaatta catcagcctg
     5221 ttccggaaga aaaccagaac cgagaaggag aataaggagc tggagaacct ggagatcaat
     5281 ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg gctacaagtc cctgtttaag
     5341 aaggatatca tcgagacaat cctgccagag ttcctggacg ataaggacga gatcgccctg
     5401 gtgaacagct tcaatggctt taccacagcc ttcaccggct tctttgataa cagagagaat
     5461 atgttttccg aggaggccaa gagcacatcc atcgccttca ggtgtatcaa cgagaatctg
     5521 acccgctaca tctctaatat ggacatcttc gagaaggtgg acgccatctt tgataagcac
     5581 gaggtgcagg agatcaagga gaagatcctg aacagcgact atgatgtgga ggatttcttt
     5641 gagggcgagt tctttaactt tgtgctgaca caggagggca tcgacgtgta taacgccatc
     5701 atcggcggct tcgtgaccga gagcggcgag aagatcaagg gcctgaacga gtacatcaac
     5761 ctgtataatc agaaaaccaa gcagaagctg cctaagttta agccactgta taagcaggtg
     5821 ctgagcgatc gggagtctct gagcttctac ggcgagggct atacatccga tgaggaggtg
     5881 ctggaggtgt ttagaaacac cctgaacaag aacagcgaga tcttcagctc catcaagaag
     5941 ctggagaagc tgttcaagaa ttttgacgag tactctagcg ccggcatctt tgtgaagaac
     6001 ggccccgcca tcagcacaat ctccaaggat atcttcggcg agtggaacgt gatccgggac
     6061 aagtggaatg ccgagtatga cgatatccac ctgaagaaga aggccgtggt gaccgagaag
     6121 tacgaggacg atcggagaaa gtccttcaag aagatcggct ccttttctct ggagcagctg
     6181 caggagtacg ccgacgccga tctgtctgtg gtggagaagc tgaaggagat catcatccag
     6241 aaggtggatg agatctacaa ggtgtatggc tcctctgaga agctgttcga cgccgatttt
     6301 gtgctggaga agagcctgaa gaagaacgac gccgtggtgg ccatcatgaa ggacctgctg
     6361 gattctgtga agagcttcga gaattacatc aaggccttct ttggcgaggg caaggagaca
     6421 aacagggacg agtccttcta tggcgatttt gtgctggcct acgacatcct gctgaaggtg
     6481 gaccacatct acgatgccat ccgcaattat gtgacccaga agccctactc taaggataag
     6541 ttcaagctgt attttcagaa ccctcagttc atgggcggct gggacaagga taaggagaca
     6601 gactatcggg ccaccatcct gagatacggc tccaagtact atctggccat catggataag
     6661 aagtacgcca agtgcctgca gaagatcgac aaggacgatg tgaacggcaa ttacgagaag
     6721 atcaactata agctgctgcc cggccctaat aagatgctgc caaaggtgtt cttttctaag
     6781 aagtggatgg cctactataa ccccagcgag gacatccaga agatctacaa gaatggcaca
     6841 ttcaagaagg gcgatatgtt taacctgaat gactgtcaca agctgatcga cttctttaag
     6901 gatagcatct cccggtatcc aaagtggtcc aatgcctacg atttcaactt ttctgagaca
     6961 gagaagtata aggacatcgc cggcttttac agagaggtgg aggagcaggg ctataaggtg
     7021 agcttcgagt ctgccagcaa gaaggaggtg gataagctgg tggaggaggg caagctgtat
     7081 atgttccaga tctataacaa ggacttttcc gataagtctc acggcacacc caatctgcac
     7141 accatgtact tcaagctgct gtttgacgag aacaatcacg gacagatcag gctgagcgga
     7201 ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg aggagctggt ggtgcaccca
     7261 gccaactccc ctatcgccaa caagaatcca gataatccca agaaaaccac aaccctgtcc
     7321 tacgacgtgt ataaggataa gaggttttct gaggaccagt acgagctgca catcccaatc
     7381 gccatcaata agtgccccaa gaacatcttc aagatcaata cagaggtgcg cgtgctgctg
     7441 aagcacgacg ataaccccta tgtgatcggc atcgataggg gcgagcgcaa tctgctgtat
     7501 atcgtggtgg tggacggcaa gggcaacatc gtggagcagt attccctgaa cgagatcatc
     7561 aacaacttca acggcatcag gatcaagaca gattaccact ctctgctgga caagaaggag
     7621 aaggagaggt tcgaggcccg ccagaactgg acctccatcg agaatatcaa ggagctgaag
     7681 gccggctata tctctcaggt ggtgcacaag atctgcgagc tggtggagaa gtacgatgcc
     7741 gtgatcgccc tggaggacct gaactctggc tttaagaata gccgcgtgaa ggtggagaag
     7801 caggtgtatc agaagttcga gaagatgctg atcgataagc tgaactacat ggtggacaag
     7861 aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct atcagatcac caataagttc
     7921 gagagcttta agtccatgtc tacccagaac ggcttcatct tttacatccc tgcctggctg
     7981 acatccaaga tcgatccatc taccggcttt gtgaacctgc tgaaaaccaa gtataccagc
     8041 atcgccgatt ccaagaagtt catcagctcc tttgacagga tcatgtacgt gcccgaggag
     8101 gatctgttcg agtttgccct ggactataag aacttctctc gcacagacgc cgattacatc
     8161 aagaagtgga agctgtactc ctacggcaac cggatcagaa tcttccggaa tcctaagaag
     8221 aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg cctataagga gctgttcaac
     8281 aagtacggca tcaattatca gcagggcgat atcagagccc tgctgtgcga gcagtccgac
     8341 aaggccttct actctagctt tatggccctg atgagcctga tgctgcagat gcggaacagc
     8401 atcacaggcc gcaccgacgt ggattttctg atcagccctg tgaagaactc cgacggcatc
     8461 ttctacgata gccggaacta tgaggcccag gagaatgcca tcctgccaaa gaacgccgac
     8521 gccaatggcg cctataacat cgccagaaag gtgctgtggg ccatcggcca gttcaagaag
     8581 gccgaggacg agaagctgga taaggtgaag atcgccatct ctaacaagga gtggctggag
     8641 tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca cgaaaaaggc cggccaggca
     8701 aaaaagaaaa agggatccgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac
     8761 gtcgaggaga atcctggccc agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     8821 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     8881 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     8941 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     9001 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     9061 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     9121 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     9181 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     9241 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     9301 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9361 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     9421 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     9481 gacgagctgt acaagtaaaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
     9541 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     9601 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9661 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9721 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     9781 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     9841 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9901 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9961 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10021 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10081 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10141 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10201 tataagggat tttgccgatt tcggcctat
//
