LOCUS       sequence-196930-        7157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_ZNF511
FEATURES             Location/Qualifiers
     source          1..7157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             840..863
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             867..890
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             894..917
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             933..989
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1002..1796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1056..1075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1666..1685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2659..2675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2668..2690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2894..3709)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3617..3636
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3701..3720
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4089..4677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4578..4597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4831..4848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5148..5169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5184..5189,5190..5207,5208..5214)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5222..5238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5227..5249
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5246..5262
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5246..5262
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5439..5641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5592..5612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5638..5662
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5738..6868
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
ORIGIN
        1 gaatgccaag caccactcac ttttcctcat tgtctgagtg ggatggaaag agtctcactt
       61 gcagttgctt cagtcacaac ccaggcgtct gccttaatag catcacctgt gccgtctgcg
      121 tttctagcgt cacctgtgcc gtctgcgttt ctagcgtcac ctgtgccgtc tgccttccta
      181 gcatgacctg tgccgtctgc ctttctagca tgacctgtac tgtctgcctt tctagcgtca
      241 cctgtgccgt ctgcctttct agttaaagtt gattttaaat taatttggga aaaacggtga
      301 acaagtgggg aaaaacatca ccaaagactc caaacaggtg gaacgatgaa cttaatgttc
      361 cctgcaccct ccttacagat gcctggactt tggcctgtga atgagccccg gggggctggg
      421 acagtagggg ttttcatgga atggacatgg ggtagcagga agtctcagcc ccagagggct
      481 tggcaggagc cgccctctct gcggagttct tcccagcggg tgtgcgttgg tggctggcgg
      541 ggacccccca caggaactag gtctcaggct gtggaaaggg agctgcccca gggcccagga
      601 gggccggctc tgcattccgc atgttcccag agtgaaagag aatgagaagg agacacacaa
      661 gtgctgttat gacctgggtt gttggttggc aaatacagca tctaatagaa actttcttcc
      721 atcccagaat accctctacc atctgctttg gtcagggtgc cgctcgagga tttaaaagca
      781 acaagaagaa aaccaaacaa tgcgggagcg gaggaggttc cggtggaggt ggttctggag
      841 attacaagga tgacgacgat aagggcgatt acaaggatga cgacgataag ggagattaca
      901 aggatgacga cgataaggtt tcaggaagcg gagctactaa cttcagcctg ctgaagcagg
      961 ctggagacgt ggaggagaac cctggacctg gatcgtttcg catgattgaa caagatggat
     1021 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     1081 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     1141 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     1201 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     1261 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     1321 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     1381 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     1441 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     1501 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     1561 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     1621 tcgactgtgg ccggctgggt gtggccgacc gctatcagga catagcgttg gctacccgtg
     1681 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     1741 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgattcg
     1801 aacatcgtgg ggggcagtca ccactgctgg gcgtggcatc cgccttgggc ctttctccga
     1861 ccttctggtg tggccgccct gggggccagg ccctcgccat cctccccatc cttgttcctc
     1921 agcaaatggc attagcagct gtcatcagaa gctgtgaggg acccacagat tttggaaacg
     1981 acctggacac actattggga aggagatgtg gacggcctgt ctcctcctgc agggcccacc
     2041 ctaagaatgt atttttaaac acatgaaata agtatttttc actgatggtc ttgagtcttc
     2101 atttctgtgt tgagtcaggt gcggagtggt tattgttacc catgggtatt ctgttttcca
     2161 ccctcagttt tcaggaagat ttataaagtc ctggcacccc tcagaggtgg gtgatggggt
     2221 aggatacagc actccacctc gtcccggacc tcctggcttt gggagccaca cccagttgtc
     2281 cttgccacac cacttaggga gtagacctgg tgggggtagg atctgatgcc cacagggagg
     2341 ggcactggct ctgggggcat ggggtgcttg ggggtgtgtg ggatgctggg ggagtggggg
     2401 gtgctgcccc agagggtgca ggatccatca ctagtgccag cggggaccac agagcctgca
     2461 tcaggaaatg caggccagga gattggggct ggcattggat tgcagagata cactgggggc
     2521 agtgtgtcct ggagaaatca ctctgttttg ataaaattag ggcgctcaga gactcccgac
     2581 cacgtcttcc tccagcggcc ctgttccttg gaaagtcctc tccactgact gtagcctcca
     2641 attcactggc cgtcgttcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2701 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2761 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgcgattt
     2821 attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
     2881 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     2941 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     3001 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     3061 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     3121 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     3181 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     3241 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     3301 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     3361 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     3421 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     3481 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     3541 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     3601 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     3661 aatttaatcg cggcttcgag caagacgttt cccgttgaat atggctcata acaccccttg
     3721 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     3781 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg
     3841 ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc
     3901 aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc
     3961 tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt
     4021 cacgaggcag acctctcgac ggagttccac tgagcgtcag accccgtaga aaagatcaaa
     4081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagaa
     4861 gctcgcacat tcagcagcgt ttttcagcgc gttttcgatc aacgtttcaa tgttggtatc
     4921 aacaccaggt ttaactttga acttatcggc actgacggtt actgattttg aacttttgct
     4981 ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa
     5041 aagttcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5101 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5161 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5221 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     5281 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt agcttgcatg
     5341 cctgcaggtc aattccctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5401 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5461 tcaatgggcg tggatagcgg tttgactcac ggggatttca agtctccacc ccattgacgt
     5521 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     5581 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5641 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     5701 gagacaccgg gaccgatcca gcctggggat cccgaccatg gcttcgtacc cctgccatca
     5761 acacgcgtct gcgttcgacc aggctgcgcg ttctcgcggc catagcaacc gacgtacggc
     5821 gttgcgccct cgccggcagc aagaagccac ggaagtccgc ctggagcaga aaatgcccac
     5881 gctactgcgg gtttatatag acggtcctca cgggatgggg aaaaccacca ccacgcaact
     5941 gctggtggcc ctgggttcgc gcgacgatat cgtctacgta cccgagccga tgacttactg
     6001 gcaggtgctg ggggcttccg agacaatcgc gaacatctac accacacaac accgcctcga
     6061 ccagggtgag atatcggccg gggacgcggc ggtggtaatg acaagcgccc agataacaat
     6121 gggcatgcct tatgccgtga ccgacgccgt tctggctcct catatcgggg gggaggctgg
     6181 gagctcacat gccccgcccc cggccctcac cctcatcttc gaccgccatc ccatcgccgc
     6241 cctcctgtgc tacccggccg cgcgatacct tatgggcagc atgacccccc aggccgtgct
     6301 ggcgttcgtg gccctcatcc cgccgacctt gcccggcaca aacatcgtgt tgggggccct
     6361 tccggaggac agacacatcg accgcctggc caaacgccag cgccccggcg agcggcttga
     6421 cctggctatg ctggccgcga ttcgccgcgt ttacgggctg cttgccaata cggtgcggta
     6481 tctgcagggc ggcgggtcgt ggcgggagga ttggggacag ctttcgggga cggccgtgcc
     6541 gccccagggt gccgagcccc agagcaacgc gggcccacga ccccatatcg gggacacgtt
     6601 atttaccctg tttcgggccc ccgagttgct ggcccccaac ggcgacctgt acaacgtgtt
     6661 tgcctgggcc ttggacgtct tggccaaacg cctccgtccc atgcacgtct ttatcctgga
     6721 ttacgaccaa tcgcccgccg gctgccggga cgccctgctg caacttacct ccgggatggt
     6781 ccagacccac gtcaccaccc ccggctccat accgacgatc tgcgacctgg cgcgcacgtt
     6841 tgcccgggag atgggggagg ctaactgagg atcccacgtc actattgtat actctatatt
     6901 atactctatg ttatactctg taatcctact caataaacgt gtcacgcctg tgaaaccgta
     6961 ctaagtctcc cgtgtattct tatcaccatc aggtgacatc ctcgcccagg ctgtcaatca
     7021 tgccggtatc gattccagta gcaccggccc cacgctgaca acccactctt gcagcgttag
     7081 cagcgcccct cttaacaagc cgacccccac cagcgtcgcg gttactaaca ctcctctccc
     7141 cgacctgcag cccagct
//
