LOCUS       sequence-196954-       14205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 14205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..14205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1221..1243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1235..1252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1236..1252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1295..2377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIDVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGYWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2339..2358
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    2761..2785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4463..4480
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        12282..12386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12387..12455,12456..13247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12605..12624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13418..14006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13907..13926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14160..14177
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      121 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc tgcatgataa
      181 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      241 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      301 aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc attttcaggt
      361 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      421 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      481 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      541 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      601 ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa aacactaata
      661 tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt aaatatctct
      721 tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc ctcctaaatt
      781 tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga atcaatcctt
      841 ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc
      901 aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa
      961 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
     1021 atcttgataa taagggtaac tattgccgtc gtccattccg acagcatcgc cagtcactat
     1081 ggcgtgctgc tagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
     1261 gctcaggcct taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     1441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     1501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     1561 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     1621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     1681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtagccttcc cgttccgcta
     1741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     1801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2041 tgtgcaccgc cgttttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2161 gggccagact ggaggtggca acgtcaatca gcaacgactg tttgcccgcc agttgttgtg
     2221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     2341 catactctgc gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga
     2401 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
     2461 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
     2521 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
     2581 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
     2641 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
     2701 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
     2761 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatccatga gacgcagacg
     2821 cagaagatct ctgactgagg aaaaaccaat ccgtccgatc gcaaactttc cgccgagcat
     2881 ctggggtgat cagttcctga tctaccagaa gcaggtcgag caaggcgtag aacagatcgt
     2941 gaacgacctg aaaaaggagg tacgtcagct gctgaaagag gctctggaca tcccgatgaa
     3001 acatgcgaac ctgctgaagc tgattgatga gattcagcgc ctgggtatcc cataccactt
     3061 cgaacgtgaa attgatcacg cgctgcagtg tatttatgaa acgtatggtg ataactggaa
     3121 cggtgaccgt agctccctgt ggttccgtct gatgcgtaag cagggttatt acgtgacctg
     3181 cgacgtcttc aacaactaca aagacaagaa cggcgcgttt aaacagagcc tggcgaatga
     3241 cgttgaaggc ctgctggagc tgtatgaagc aacctctatg cgtgttccgg gcgaaatcat
     3301 cctggaagat gctctgggtt tcacccgttc tcgcctgtct atcatgacca aggacgcatt
     3361 ttccactaac ccggccctgt tcaccgaaat ccagcgtgcg ctgaaacagc ctctgtggaa
     3421 gcgtctgccg cgtatcgagg cggcacagta catcccgttc tatcagcaac aggattccca
     3481 caacaaaacc ctgctgaaac tggcgaaact ggaatttaac ctgctgcaat ccctgcacaa
     3541 agaagaactg tctcatgttt gcaaatggtg gaaagccttc gacatcaaga aaaacgcgcc
     3601 gtgcctgcgc gaccgtatcg tagaatgcta tttctggggc ctgggctccg gttatgagcc
     3661 tcaatactct cgtgctcgcg tattcttcac caaagcggta gctgtgatca ccctgatcga
     3721 cgatacctac gatgcttacg gtacgtacga agaactgaag atttttaccg aggctgttga
     3781 acgctggtct atcacttgcc tggataccct gccggaatac atgaaaccga tctataaact
     3841 gttcatggat acttataccg aaatggaaga gttcctggca aaagaaggtc gcactgatct
     3901 gttcaactgt ggcaaggagt ttgtgaaaga attcgtccgc aacctgatgg tggaggcgaa
     3961 gtgggcaaac gagggtcaca tcccgaccac cgaagaacac gacccggtag ttatcatcac
     4021 cggtggcgca aacctgctga ctaccacttg ctacctgggt atgtccgaca tttttacgaa
     4081 agaaagcgtt gaatgggcag tgtctgcgcc tccgctgttc cgttactctg gtatcctggg
     4141 ccgtcgcctg aacgatctga tgacccataa agccgagcag gaacgtaaac actccagcag
     4201 ctctctggaa tcttacatga aagaatacaa cgttaacgaa gagtatgcgc agaccctgat
     4261 ctacaaagag gttgaggatg tatggaaaga catcaaccgt gagtacctga ctactaaaaa
     4321 catcccacgc ccgctgctga tggcagtgat ttatctgtgc cagtttctgg aagtgcagta
     4381 tgcgggcaaa gataacttca cccgtatggg tgacgaatac aaacacctga tcaagtccct
     4441 gctggtttac ccaatgtcca tccatcatca tcaccatcac tgatctagag tcgacgtccc
     4501 cggggcagcc cgcctaatga gcgggctttt ttcacgtcac gcgtccatgg agatctttgt
     4561 ctgcaactga aaagtttata ccttacctgg gacataacac tcttaataga agagggtaat
     4621 tctttctctt ttatagaaaa tcaattaatt gaaagtagct ccttcattct taagatcaaa
     4681 cgtgatatag tttgctaacc tttgcgttca cttaactaac ttataggggt aacacttaaa
     4741 aaagaatcaa taacgataga aaccgctcct aaagcaggtg cattttttcc taacgaagaa
     4801 ggcaatagtt cacatttatt gtctaaatga gaatggactc tagaagaaac ttcgttttta
     4861 atcgtattta aaacaatggg atgagattca attatatgat ttctcaagat aacagcttct
     4921 atatcaaatg tattaaggat attggttaat ccaattccga tataaaagcc aaagttttga
     4981 agtgcattta acatttctac atcattttta tttgcgcgtt ccacaatctc ttttcgagaa
     5041 atattctttt cttctttaga gagcgaagcc agtaacgctt tttcagaagc atataattcc
     5101 caacagcctc gatttccaca gctgcatttg ggtccattaa aatctatcgt catatgaccc
     5161 atttccccag aaaaaccctg aacaccttta tacaattcgt tgttaataac aagtccagtt
     5221 ccaattccga tattaatact gatgtaaacg atgttttcat agttttttgt cataccaaat
     5281 actttttcac cgtatgctcc tgcattagct tcattttcaa caaaaaccgg aacattaaac
     5341 tcactctcaa ttaaaaactg caaatctttg atattccaat ttaagttagg catgaaaata
     5401 atttgctgat gacgatctac aaggcctgga acacaaattc ctattccgac tagaccataa
     5461 ggggactcag gcatatgggt tacaaaacca tgaataagtg caaataaaat ctcttttact
     5521 tcactagcgg aagaactaga caagtcagaa gtcttctcga gaataatatt tccttctaag
     5581 tcggttagaa ttccgttaag atagtcgact cctatatcaa taccaatcga gtagcctgca
     5641 ttcttattaa aaacaagcat tacaggtctt ctgccgcctc tagattgccc tgccccaatt
     5701 tcaaaaataa aatctttttc aagcagtgta tttacttgag aggagacagt agacttgttt
     5761 aatcctgtaa tctcagagag agttgccctg gagacagggg agttcttcaa aatttcatct
     5821 aatattaatt tttgattcat tttttttact aaagcttgat ctgcaatttg aataataacc
     5881 actcctttgt ttatccaccg aactaagttg gtgttttttg aagcttgaat tagatattta
     5941 aaagtatcat atctaatatt ataactaaat tttctaaaaa aaacattgaa ataaacattt
     6001 attttgtata tgatgagata aagttagttt attggataaa caaactaact caattaagat
     6061 agttgatgga taaacttgtt cacttaaatc aaagggggaa atgacaaatg gtccaaacta
     6121 gtgatctttt atcaatacag gacccgtcgt ttttaaaaaa catgtccatt gatgaattag
     6181 agaaattaag tgatgaaatc cgtcagtttt taattacaag tttatccgct tccggcggcc
     6241 acatcggccc aaacttaggt gtcgtagagc ttactgttgc cctgcataag gaatttaaca
     6301 gcccgaaaga caaattttta tgggatgtag gccatcagtc gtatgtccat aagctgctga
     6361 caggacgcgg aaaagaattt gcgacgcttc gccagtacaa agggctttgc ggatttccaa
     6421 agcggagtga aagcgagcac gatgtttggg aaaccgggca cagctcgact tctctgtcag
     6481 gcgcgatggg aatggcagct gcccgtgata ttaaaggaac ggatgaatat attattccga
     6541 tcattggtga cggcgcgctg accggcggta tggcgctcga agcccttaac cacatcggcg
     6601 acgagaaaaa agacatgatt gtcatcctta atgataatga aatgagtatt gcgccaaacg
     6661 tcggtgccat tcactctatg ctcggacggc tccgcactgc ggggaaatac cagtgggtca
     6721 aagatgagct tgaatactta tttaaaaaga ttccggcagt tgggggcaag cttgccgcca
     6781 cggcggaacg ggtcaaagac agcctgaaat acatgctcgt ctccggaatg tttttcgagg
     6841 agctcggttt tacgtatttg ggcccagtgg acggacattc ttatcatgag ctgattgaga
     6901 atcttcaata cgccaaaaaa acgaaaggcc ctgttcttct gcacgtcatt acgaaaaaag
     6961 ggaaggggta caaaccggct gagaccgata cgattgggac atggcatggt accggaccat
     7021 ataaaattaa taccggtgac tttgtaaagc cgaaagccgc agctccttcg tggagcggtc
     7081 ttgtcagcgg aactgtgcag cgaatggcgc acgaggacgg acgcattgta gccattacgc
     7141 cggctatgcc tgtcggttca aagcttgaag gcttcgcaaa ggaattccct gaccggatgt
     7201 tcgacgtagg aatcgcagaa cagcatgccg caacaatggc tgcagctatg gcaatgcagg
     7261 gtatgaagcc gtttttggcg atttactcaa ccttcctgca aagggcatat gaccaagttg
     7321 ttcatgacat ctgccgccaa aacgctaatg tgtttattgg aattgaccgt gctggactcg
     7381 ttggcgctga tggagagaca catcaaggcg tgtttgatat tgcgtttatg cgccacattc
     7441 caaacatggt cttaatgatg ccgaaagacg aaaatgaagg ccagcacatg gttcatacag
     7501 cacttagcta tgacgaaggc ccgatagcaa tgcgttttcc gcgcggaaac ggactcggcg
     7561 taaaaatgga tgaacagttg aaaacgattc cgatcggtac gtgggaggtg ctgcgtccag
     7621 ggaacgatgc tgtcatctta acattcggca caacaatcga aatggcgatt gaagcagccg
     7681 aagagctgca gaaagaaggc ctttccgtgc gcgttgtgaa tgcgcgtttt attaagccga
     7741 ttgatgaaaa gatgatgaag agtatcctaa aagaaggctt gccaatttta acaattgaag
     7801 aagcggtctt agaaggcggt ttcggaagct cgattttaga attcgctcat gatcaaggtg
     7861 aatatcatac tccgattgac agaatgggta tacctgatcg gtttattgaa cacggaagtg
     7921 taacagcgct tcttgaggaa attggactga caaaacagca ggtggcaaat cgtattagat
     7981 tactgatgcc accaaagaca cacaaaggaa ttggatcatg aggatccaaa ggaggaagga
     8041 accatgactc gagcagaacg aaaaagacaa cacatcaatc atgccttgtc catcggccag
     8101 aagcgggaaa caggtcttga tgatattacg tttgttcacg tcagtctgcc cgatcttgca
     8161 ttagaacaag tagatatttc cacaaaaatc ggcgaacttt caagcagttc gccgattttt
     8221 atcaatgcaa tgactggcgg cggcggaaaa cttacatatg agattaataa atcgcttgcg
     8281 cgagcggctt ctcaggctgg aattcccctt gctgtgggat cgcaaatgtc agcattaaaa
     8341 gatccatcag agcgtctttc ctatgaaatt gttcgaaagg aaaacccaaa cgggctgatt
     8401 tttgccaacc tgggaagcga ggcaacggct gctcaggcaa aggaagccgt tgagatgatt
     8461 ggagcaaacg cactgcagat ccacctcaat gtgattcagg aaattgtgat gcctgaaggg
     8521 gacagaagct ttagcggcgc attgaaacgc attgaacaaa tttgcagccg ggtcagtgta
     8581 ccggtcattg tgaaagaagt cggcttcggt atgagcaaag catcagcagg aaagctgtat
     8641 gaagctggtg ctgcagctgt tgacattggc ggttacgggg gaacaaattt ctcgaaaatc
     8701 gaaaatctcc gaagacagcg gcaaatctcc ttttttaatt cgtggggcat ttcgacagct
     8761 gcaagtttgg cggaaatccg ctctgagttt cctgcaagca ccatgatcgc ctctggcggt
     8821 ctgcaagatg cgcttgacgt ggcaaaggca attgcgctgg gggcctcttg caccggaatg
     8881 gcagggcatt ttttaaaagc gctgactgac agcggtgagg aaggactgct tgaggagatt
     8941 cagctgatcc ttgaggaatt aaagttgatt atgaccgtgc tgggtgccag aacaattgcc
     9001 gatttacaaa aggcgcccct tgtgatcaaa ggtgaaaccc atcattggct cacagagaga
     9061 ggggtcaata catcaagcta tagtgtgcga taaagatcta gatatcgatg aattgatccg
     9121 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccaacaa atggttgaaa
     9181 catacgaggc taatatcggc ttattaggaa tagtccctgt actaataaaa tcaggtggat
     9241 cagttgatca gtatattttg gacgaagctc ggaaagaatt tggagatgac ttgcttaatt
     9301 ccacaattaa attaagggaa agaataaagc gatttgatgt tcaaggaatc acggaagaag
     9361 atactcatga taaagaagct ctaaaactat tcaataacct tacaatggaa ttgatcgaaa
     9421 gggtggaagg ttaatggtac gaaaattagg ggatctacct agaaagccac aaggcgatag
     9481 gtcaagctta aagaaccctt acatggatct tacagattct gaaagtaaag aaacaacaga
     9541 ggttaaacaa acagaaccaa aaagaaaaaa agcattgttg aaaacaatga aagttgatgt
     9601 ttcaatccat aataagatta aatcgctgca cgaaattctg gcagcatccg aagggaattc
     9661 atattactta gaggatacta ttgagagagc tattgataag atggttgaga cattacctga
     9721 gagccaaaaa actttttatg aatatgaatt aaaaaaaaga accaacaaag gctgagacag
     9781 actccaaacg agtctgtttt tttaaaaaaa atattaggag cattgaatat atattagaga
     9841 attaagaaag acatgggaat aaaaatattt taaatccagt aaaaatatga taagattatt
     9901 tcagaatatg aagaactctg tttgtttttg atgaaaaaac aaacaaaaaa aatccaccta
     9961 acggaatctc aatttaacta acagcggcca aactgagaag ttaaatttga gaaggggaaa
    10021 aggcggattt atacttgtat ttaactatct ccattttaac attttattaa accccataca
    10081 agtgaaaatc ctcttttaca ctgttccttt aggtgatcgc ggagggacat tatgagtgaa
    10141 gtaaacctaa aaggaaatac agatgaatta gtgtattatc gacagcaaac cactggaaat
    10201 aaaatcgcca ggaagagaat caaaaaaggg aaagaagaag tttattatgt tgctgaaacg
    10261 gaagagaaga tatggacaga agagcaaata aaaaactttt ctttagacaa atttggtacg
    10321 catatacctt acatagaagg tcattataca atcttaaata attacttctt tgatttttgg
    10381 ggctattttt taggtgctga aggaattgcg ctctatgctc acctaactcg ttatgcatac
    10441 ggcagcaaag acttttgctt tcctagtcta caaacaatcg ctaaaaaaat ggacaagact
    10501 cctgttacag ttagaggcta cttgaaactg cttgaaaggt acggttttat ttggaaggta
    10561 aacgtccgta ataaaaccaa ggataacaca gaggaatccc cgatttttaa gattagacgt
    10621 aaggttcctt tgctttcaga agaactttta aatggaaacc ctaatattga aattccagat
    10681 gacgaggaag cacatgtaaa gaaggcttta aaaaaggaaa aagagggtct tccaaaggtt
    10741 ttgaaaaaag agcacgatga atttgttaaa aaaatgatgg atgagtcaga aacaattaat
    10801 attccagagg ccttacaata tgacacaatg tatgaagata tactcagtaa aggagaaatt
    10861 cgaaaagaaa tcaaaaaaca aatacctaat cctacaacat cttttgagag tatatcaatg
    10921 acaactgaag aggaaaaagt cgacagtact ttaaaaagcg aaatgcaaaa tcgtgtctct
    10981 aagccttctt ttgatacctg gtttaaaaac actaagatca aaattgaaaa taaaaattgt
    11041 ttattacttg taccgagtga atttgcattt gaatggatta agaaaagata tttagaaaca
    11101 attaaaacag tccttgaaga agctggatat gttttcgaaa aaatcgaact aagaaaagtg
    11161 caataaactg ctgaagtatt tcagcagttt tttttattta gaaatagtga aaaaaatata
    11221 atcagggagg tatcaatatt taatgagtac tgatttaaat ttatttagac tggaattaat
    11281 aattaacacg tagactaatt aaaatttaat gagggataaa gaggatacaa aaatattaat
    11341 ttcaatccct attaaatttt aacaaggggg ggattaaaat ttaattagag gtttatccac
    11401 aagaaaagac cctaataaaa tttttactag ggttataaca ctgattaatt tcttaatggg
    11461 ggagggatta aaatttaatg acaaagaaaa caatctttta agaaaagctt ttaaaagata
    11521 ataataaaaa gagctttgcg attaagcaaa actctttact ttttcattga cattatcaaa
    11581 ttcatcgatt tcaaattgtt gttgtatcat aaagttaatt ctgttttgca caaccttttc
    11641 aggaatataa aacacatctg aggcttgttt tataaactca gggtcgctaa agtcaatgta
    11701 acgtagcata tgatatggta tagcttccac ccaagttagc ctttctgctt cttctgaatg
    11761 tttttcatat acttccatgg gtatctctaa atgattttcc tcatgtagca aggtatgagc
    11821 aaaaagttta tggaattgat agttcctctc tttttcttca acttttttat ctaaaacaaa
    11881 cactttaaca tctgagtcaa tgtaagcata agatgttttt ccagtcataa tttcaatccc
    11941 aaatctttta gacagaaatt ctggacgtaa atcttttggt gaaagaattt ttttatgtag
    12001 caatatatcc gatacagcac cttctaaaag cgttggtgaa tagggcattt tacctatctc
    12061 ctctcatttt gtggaataaa aatagtcata ttcgtccatc tacctatcct attatcgaac
    12121 agttgaactt tttaatcaag gatcagtcct ttttttcatt attcttaaac tgtgctctta
    12181 actttaacaa ctcgatttgt ttttccagat ctcgagggta actagcctcg ccgatcccgc
    12241 aagaggcccg gcagtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    12301 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    12361 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    12421 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    12481 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    12541 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    12601 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    12661 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    12721 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    12781 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    12841 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    12901 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    12961 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    13021 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    13081 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    13141 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    13201 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    13261 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    13321 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    13381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    13441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    13501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    13561 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    13621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    13681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    13741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    13801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    13861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    13921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    13981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    14041 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    14101 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    14161 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg catgc
//
