LOCUS       sequence-196960-        5866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p57 promoter reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Yeh HW, Hsu EC, Lee SS, Lang YD, Lin YC, Chang CY, Lee SY, Gu DL, 
            Shih JH, Ho CM, Chen CF, Chen CT, Tu PH, Cheng CF, Chen RH, Yang RB,
            Jou YS
  TITLE     PSPC1 mediates TGF-beta1 autocrine signalling and Smad2/3 target 
            switching to promote EMT, stemness and metastasis.
  JOURNAL   Nat Cell Biol. 2018 Apr;20(4):479-491. doi: 
            10.1038/s41556-018-0062-y. Epub 2018 Mar 28.
  PUBMED    29593326
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-018-0062-y"; journalName: "Nat Cell Biol"; date: 
            "2018-04"; volume: "20"; issue: "4"; pages: "479-491"
COMMENT     Sequence Label: pGL3-p57
FEATURES             Location/Qualifiers
     source          1..5866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1137..1146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1143..2795
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1209..1227)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2836..2957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2847..2866)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2901..2920
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3205..3222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3376..3964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3456..3475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4135..4926,4927..4995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4758..4777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4996..5100)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5127..5582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5214..5233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5424..5445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5713..5761
                     /note="synthetic polyadenylation signal"
     misc_feature    5775..5866
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5815..5834
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 atcgataggt acctacccag tacaacagct tccagccagc ctggacaggc agatcccggg
       61 gctggagtgg tctggcccaa ggctgagaag ggctgggacc cccgacagtg acatgatggg
      121 ctcagcccct gttccatcta ctgggcaggg tgggcctccg cagatagcgg cttcagactc
      181 cagctccagg gggctggtgg gagtctctca aggtgtccca cacatggtcc ctcggggaaa
      241 gagtggagct gacccctgtc ccccctcccg actcgagggc cttagggcca gcaggtgtgt
      301 gagggctcca ggcgcgcgga gccctcgaag ggacaccagc ccagcagcag caggccgcgc
      361 gggggcagcc gaagacccca tccggcgcag gccagggccg agctggcagc ggcgggtcca
      421 agcctcgtca gctggcgcag gaggcccacg ggcgagcgcg ggacggagct gggcgccggg
      481 gtccggaccg ggccggggtc gagttgggcc tggccggaca ccggacccgc caggagccgg
      541 cccctgccgc gctgggctga ggcccccgcc tgcagacaaa ggagccggcg gggggcggga
      601 gccggggggg tgggggagtg ttgcaggggg gatggggcga gggggcgggc gtcgcggtgt
      661 cacgttaccg cccgcagcgc cctttaactc ccgcccccgc cccgctcacc gcccccttcc
      721 cctgctacag cgcgctcgcc ccgcggccgc caatcagccg cgcgccccgg ccgcgcgccc
      781 cgccccgccc ccggtgggtg tgcgcgcggc caatgggcgg tgcgcggggg ccgggccgcg
      841 gcggggcggg gcagcggggc ggccgccaat cgccgtggtg ttgttgaaac tgaaaatact
      901 acattatgct aatcgcggcc gggcccgcgc gcacgggggt ggggcccgcg cgtataaagg
      961 gggcgcaggc gggctgggcg ttccacaggc caagtgcgct gtgctcgagg ggtgccggcc
     1021 aggcctgagc gagcgagcta gccagcaggc atcgaggggg cgcggctgcc gtccggacga
     1081 gacaggcgaa cccgacgcag aagagtcaag cttggcattc cggtactgtt ggtaaagcca
     1141 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
     1201 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     1261 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
     1321 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     1381 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     1441 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
     1501 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     1561 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
     1621 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     1681 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     1741 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     1801 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     1861 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     1921 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     1981 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     2041 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     2101 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     2161 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     2221 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     2281 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     2341 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     2401 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     2461 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     2521 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     2581 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     2641 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     2701 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     2761 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc
     2821 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2881 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2941 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3001 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     3061 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     3121 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     3181 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5101 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     5161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5521 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     5581 ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5641 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
     5701 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     5761 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     5821 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
