LOCUS       sequence-196961-        5101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E.coli/H.Pylori shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Heuermann D, Haas R
  TITLE     A stable shuttle vector system for efficient genetic complementation
            of Helicobacter pylori strains by transformation and conjugation.
  JOURNAL   Mol Gen Genet. 1998 Mar;257(5):519-28.
  PUBMED    9563837
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Gen 
            Genet."; date: "1998-03"; volume: "257(5)"; pages: "519-28"
COMMENT     Sequence Label: pHel2
FEATURES             Location/Qualifiers
     source          1..5101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3012..3671
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3078..3097)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3800..3848
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      complement(4122..4710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4202..4221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            4936..5045
                     /note="incP origin of transfer"
ORIGIN
        1 agaagcttgt ccgttagcaa atataatgtg tttagcacct aaaagcgcat ctatcttgtg
       61 ttagccatct aacctaaaca aaaccgccta gcgagcgtta gcgagcatgg acaaaagcgc
      121 atcgcagttt gaaatcgtag gcgttagcag gatggggttt tgcgttagca aaatcaaaca
      181 agntagcgga aaaccggggg ggtcaccaaa aaaaacccta aaactaaaat ccccaaaata
      241 tgtagcgcgt catgcgcgtt gtttttatta catgttttaa taactatgtt gtttttacat
      301 gtttttacaa cgcgcgcgcg tgtgagggat tgggggttgc aaccccctaa ataacgaagc
      361 tgtagggttt ctcatttttg ggttaaaatg aataaaacag aacttcttac gagtgataac
      421 agaacttctt acgagtgata acagaacttc ttacgagtga taacagaact tcttacgagt
      481 gataacagaa cttctttatt gtgaagttat gattattaac aatttttagc taaaataaca
      541 acctaaggcg gtgtaacatg gttgctaatc ctagtgttaa caaatttgga gcaattagct
      601 ttaaaagcta gtgggttggg agtttgtagc gggtatgcac tccgttagga ggcacaccat
      661 gaaagcattt ttgatagtag tgattttagt ggtaatcttg acacagccac tatattaaaa
      721 ccttagcgtt ttaataaccc ttataagtcc gccaagactt cttaagggtt tcactcctgt
      781 tattatatcg tcttttgaaa aataagcatt aaaaggcgct taaatgccca tgaatacgaa
      841 ttttgaacag cttagaaaac aagaattgga attacgaaaa ttattagaag aattagaaac
      901 gctcccacaa accccacaaa ttaaactgca aaaacaaaaa atacaaactt acatagacaa
      961 gataacacca agtattttga gcggttttga tcaaaaattc aaagaaatta tagaaaatct
     1021 atcaaatgaa tttgaaaaag aaaaatccac accactcaaa gagccacaaa ccacccccac
     1081 accatgcaaa gatttagtgg ttagcacccc taaagataac acctatacca cctaccacaa
     1141 taacgctaat aaggtcaatc tagggaaatt gagcgaaagg gaagccaatc ttttattcgc
     1201 tatttttcaa aaactcaaag atcaagggaa taccctcatt cgttttgaac cgcaagattt
     1261 gaaacgcatg atcatggtca aatccaacct aaccaacaga caattattgc aaatcctcaa
     1321 aaacttactt gacaacatag gcggtgctaa tttttggatc attagagagc atgttgaaaa
     1381 tggcgaaatc tatgaagatc acactagcta catgcttttc aaacaatttg acattcgtat
     1441 ccataagcca acccaaacta tagaatactt agaagtccaa ctcaacgata gctaccacta
     1501 cctgctcaac aatctaggca tgggcggtca atacacttcc tttaaacttt tagaattcca
     1561 aagggtgagg ggcaaatacg ccaagacgct ctatcgctta ctcaagcaat acaaaagcac
     1621 agggattttg agcgtggaat gggatcaatt cagggagctt ttagacattc caaaagatta
     1681 caaaatggaa aacatcgatc aaaaagtctt aactccaagc ctcaaggaac tcagaaaaat
     1741 ctatcccttt gaacacttga gctacaaaaa agaacgcaaa agccacgaca aacgcaaggt
     1801 aacgcatatt gatttttatt ttgagcaatt gccacagggc gaaaccaaga aacaaaaaca
     1861 agccgacaag caacgcgctc aaagggacat caagctcata gcgtgggata ttcacaacca
     1921 aatcgctaaa agaaacgcta aagccactat agaagctagg tttcttgagc taaaaacctt
     1981 gattggttat cagttcaggc acaacaatgg gactatttta cagattgaca acgccacttt
     2041 tgaaaagaat caaatgtttt tgcatgtttc aaccagcaaa aattctcaaa aattccttgt
     2101 gtccaacaaa acattcgctt tagaacttct gtttgtcaat ggatacagcc taaaaaaaga
     2161 catgttggaa attgatcccc caagtatcca ccccatcacc aacgaaccta tcaaggaatt
     2221 tgcagaatac atcggaaaaa cgatccacat caccaatttc aatgtggatc aatgccctga
     2281 ggggatcaat aactacctga aaatcactag gattgtgaaa ctgaatgaca atcggatctg
     2341 tgtttcagtc caagatgtgg ataagcctga gaaacttcta aaacctttca ttgctaaaga
     2401 tgaaaaacat ttgaaaaatt ggtttaagaa acactatcgg tgaaaaataa gtggtgggat
     2461 taacaatcgt cccccacacc ttgcaatgta gttagttttg cattattttt tctataatct
     2521 ctatcaattc tgagagaaca tagattaatt caatgaaatt tttcattatc tatttccttt
     2581 ctgtaaattt tgttaaatat ttattaaaaa aaaatttaaa gatcactctt taagtggaac
     2641 atacaaaggc tgtagagagt gccacattaa gcctgatgtt ttgcttgtgt atagagtgaa
     2701 aggcaatatt ttaactttag ttaggcttgg cagtcatagt gaattgtttt agacaaattt
     2761 ctttctaaaa tcgcccagaa tgaattttta attctaattt aatataattc atcggttttc
     2821 actcaaaacg atgaaatagg gctattttta gctcattatt ggggtatttt gtttttagtt
     2881 gaaagctaac ttcaaaattc aaaaaaactt catttccctt tctccgccat attgtgttga
     2941 aacaccgccc ggaacccgat ataatccgcc cttcaacaga tccgagattt tcaggagcta
     3001 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     3061 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     3121 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     3181 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     3241 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     3301 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     3361 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     3421 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     3481 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     3541 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     3601 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     3661 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     3721 gaataagtga taataagcgg atgaatggca gaaattcgga tcttccatac ctaccagttc
     3781 tgcgcctgca ggtcgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
     3841 gagattttca acgtggatct gaattcgagc tcggtacccg gggatcctct agagtcgacc
     3901 tgcaggcatg caagctagct ttcgcgagct cgagatctag atatcgatga attcaaaaaa
     3961 aaaaggctcc aaaaggagcc tttaatgatc taattcgtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccaccggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatctctgc agttcagtaa
     4741 tttcctgcat ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg
     4801 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     4861 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     4921 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     4981 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     5041 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagctgca
     5101 g
//
