LOCUS       sequence-196962-        5509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E.coli/H.Pylori shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Heuermann D, Haas R
  TITLE     A stable shuttle vector system for efficient genetic complementation
            of Helicobacter pylori strains by transformation and conjugation.
  JOURNAL   Mol Gen Genet. 1998 Mar;257(5):519-28.
  PUBMED    9563837
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Gen 
            Genet."; date: "1998-03"; volume: "257(5)"; pages: "519-28"
COMMENT     Sequence Label: pHel3
FEATURES             Location/Qualifiers
     source          1..5509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2969..3763)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(4533..5120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4613..4632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            5346..5455
                     /note="incP origin of transfer"
ORIGIN
        1 agaagcttgt ccgttagcaa atataatgtg tttagcacct aaaagcgcat ctatcttgtg
       61 ttagccatct aacctaaaca aaaccgccta gcgagcgtta gcgagcatgg acaaaagcgc
      121 atcgcagttt gaaatcgtag gcgttagcag gatggggttt tgcgttagca aaatcaaaca
      181 agntagcgga aaaccggggg ggtcaccaaa aaaaacccta aaactaaaat ccccaaaata
      241 tgtagcgcgt catgcgcgtt gtttttatta catgttttaa taactatgtt gtttttacat
      301 gtttttacaa cgcgcgcgcg tgtgagggat tgggggttgc aaccccctaa ataacgaagc
      361 tgtagggttt ctcatttttg ggttaaaatg aataaaacag aacttcttac gagtgataac
      421 agaacttctt acgagtgata acagaacttc ttacgagtga taacagaact tcttacgagt
      481 gataacagaa cttctttatt gtgaagttat gattattaac aatttttagc taaaataaca
      541 acctaaggcg gtgtaacatg gttgctaatc ctagtgttaa caaatttgga gcaattagct
      601 ttaaaagcta gtgggttggg agtttgtagc gggtatgcac tccgttagga ggcacaccat
      661 gaaagcattt ttgatagtag tgattttagt ggtaatcttg acacagccac tatattaaaa
      721 ccttagcgtt ttaataaccc ttataagtcc gccaagactt cttaagggtt tcactcctgt
      781 tattatatcg tcttttgaaa aataagcatt aaaaggcgct taaatgccca tgaatacgaa
      841 ttttgaacag cttagaaaac aagaattgga attacgaaaa ttattagaag aattagaaac
      901 gctcccacaa accccacaaa ttaaactgca aaaacaaaaa atacaaactt acatagacaa
      961 gataacacca agtattttga gcggttttga tcaaaaattc aaagaaatta tagaaaatct
     1021 atcaaatgaa tttgaaaaag aaaaatccac accactcaaa gagccacaaa ccacccccac
     1081 accatgcaaa gatttagtgg ttagcacccc taaagataac acctatacca cctaccacaa
     1141 taacgctaat aaggtcaatc tagggaaatt gagcgaaagg gaagccaatc ttttattcgc
     1201 tatttttcaa aaactcaaag atcaagggaa taccctcatt cgttttgaac cgcaagattt
     1261 gaaacgcatg atcatggtca aatccaacct aaccaacaga caattattgc aaatcctcaa
     1321 aaacttactt gacaacatag gcggtgctaa tttttggatc attagagagc atgttgaaaa
     1381 tggcgaaatc tatgaagatc acactagcta catgcttttc aaacaatttg acattcgtat
     1441 ccataagcca acccaaacta tagaatactt agaagtccaa ctcaacgata gctaccacta
     1501 cctgctcaac aatctaggca tgggcggtca atacacttcc tttaaacttt tagaattcca
     1561 aagggtgagg ggcaaatacg ccaagacgct ctatcgctta ctcaagcaat acaaaagcac
     1621 agggattttg agcgtggaat gggatcaatt cagggagctt ttagacattc caaaagatta
     1681 caaaatggaa aacatcgatc aaaaagtctt aactccaagc ctcaaggaac tcagaaaaat
     1741 ctatcccttt gaacacttga gctacaaaaa agaacgcaaa agccacgaca aacgcaaggt
     1801 aacgcatatt gatttttatt ttgagcaatt gccacagggc gaaaccaaga aacaaaaaca
     1861 agccgacaag caacgcgctc aaagggacat caagctcata gcgtgggata ttcacaacca
     1921 aatcgctaaa agaaacgcta aagccactat agaagctagg tttcttgagc taaaaacctt
     1981 gattggttat cagttcaggc acaacaatgg gactatttta cagattgaca acgccacttt
     2041 tgaaaagaat caaatgtttt tgcatgtttc aaccagcaaa aattctcaaa aattccttgt
     2101 gtccaacaaa acattcgctt tagaacttct gtttgtcaat ggatacagcc taaaaaaaga
     2161 catgttggaa attgatcccc caagtatcca ccccatcacc aacgaaccta tcaaggaatt
     2221 tgcagaatac atcggaaaaa cgatccacat caccaatttc aatgtggatc aatgccctga
     2281 ggggatcaat aactacctga aaatcactag gattgtgaaa ctgaatgaca atcggatctg
     2341 tgtttcagtc caagatgtgg ataagcctga gaaacttcta aaacctttca ttgctaaaga
     2401 tgaaaaacat ttgaaaaatt ggtttaagaa acactatcgg tgaaaaataa gtggtgggat
     2461 taacaatcgt cccccacacc ttgcaatgta gttagttttg cattattttt tctataatct
     2521 ctatcaattc tgagagaaca tagattaatt caatgaaatt tttcattatc tatttccttt
     2581 ctgtaaattt tgttaaatat ttattaaaaa aaaatttaaa gatcactctt taagtggaac
     2641 atacaaaggc tgtagagagt gccacattaa gcctgatgtt ttgcttgtgt atagagtgaa
     2701 aggcaatatt ttaactttag ttaggcttgg cagtcatagt gaattgtttt agacaaattt
     2761 ctttctaaaa tcgcccagaa tgaattttta attctaattt aatataattc atcggttttc
     2821 actcaaaacg atgaaatagg gctattttta gctcattatt ggggtatttt gtttttagtt
     2881 gaaagctaac ttcaaaattc aaaaaaactt catttccctt tctagaggat cgatccccgg
     2941 ggatctttta gacatctaaa tctaggtact aaaacaattc atccagtaaa atataatatt
     3001 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     3061 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     3121 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     3181 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     3241 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     3301 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     3361 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     3421 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     3481 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     3541 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     3601 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     3661 ccaacttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     3721 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     3781 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     3841 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     3901 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat tatgatagaa
     3961 tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg
     4021 ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc
     4081 cttacatacg gcgatatctt ctatataagc gtaccggttc caattttttc gcagtttaac
     4141 ttttccgacg catttatcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc
     4201 tgtaaaggtc caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg
     4261 ttcgctgggt ttgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     4321 tgcaagctag ctttcgcgag ctcgagatct agatatcgat gaattcaaaa aaaaaggctc
     4381 caaaaggagc ctttaatgat ctaattcgta tcagctcact caaaggcggt aatacggtta
     4441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccg
     4801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4861 acgacttatc gccaccggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4981 ttggtatctg cgctctgctg aaaccagtta ccttcggaaa aagagttggt agctcttgat
     5041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5101 gcagaaaaaa aggatctcaa gaagatcctt tgatctctgc agttcagtaa tttcctgcat
     5161 ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg cttttccgct
     5221 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     5281 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     5341 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     5401 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc
     5461 gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagctgc
//
