LOCUS       sequence-196975-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses spoTD297A (from native promoter) from the NS1 site in the 
            S. elongatus PCC7942 genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     ppGpp Controls Global Gene Expression in Light and in Darkness in S.
            elongatus.
  JOURNAL   Cell Rep. 2017 Dec 12;21(11):3155-3165. doi: 
            10.1016/j.celrep.2017.11.067.
  PUBMED    29241543
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.11"; journalName: "Cell Rep"; date: 
            "2017-12-12- 12"; volume: "21"; issue: "11"; pages: "3155-3165"
COMMENT     Sequence Label: spoTD297A_NS1
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..108
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             5055..5846
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8000..8588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8489..8508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8742..8759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8774..8914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8840..8859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      121 tcaccgaaac gcgcgaggca ggatcgatcc ggcagccggc ggagcgctgc tttcttggca
      181 agcggtcgcc agccccaacg ccagggctgc cagcccgaaa cagcggggca aggcagcttg
      241 gaagggcgat cgcagcacgg gcatggcaat gtctctctga aggaatgcag accttattcg
      301 tacagccagg gttgaatcgt gggggtccaa tcacttagct ctgctgggct aaaccagaga
      361 gcaatttcct gttgtgctgt ttcgattgca tccgagccat ggatgatgtt gcggccaata
      421 ttgacaccaa aatcaccacg gatggtgccc ggttctgccg tcagcggatt ggtagcgccg
      481 atcaacttgc gagcagccgc cacaacgcct tcgccttcca agacgatcgc cacgatcggc
      541 ccagaggtga tgaactcgac gaggccattg aagaaggggc gctcgcggtg gacagcatag
      601 tgctgttcgg ccagctcgcg actgggcttc agctgcttta ggcccaccag tttgaagcct
      661 ttttgctcaa agcggccgat gatcgtaccg accaaacccc gctgaacgcc atcgggcttg
      721 atggcaataa atgtgcgttc cacagacatc tagatagtcc tcaagacgag gcaagcattg
      781 agcttgcctt cctatggttc gggatcactg ggattcttga caagcgatcg cggtcacatc
      841 gctatctctt aggacttcgc agcgggcgag tcggattgac ccggtaggga tttcgccaga
      901 tcaatgcccg tggtttgttt cagcttctcc agcaagctag cgatttgggt agcgctgcct
      961 tccccttcgc caatcacagt gatcgactcc acgtcgatat ctggcacggt gcctgaaagc
     1021 gtgacgagca gggactcgac ccctgctatc cgtgacattt tggtcaacag tcatgacctc
     1081 ttgaattggt agagcgcttt gcaagctcag accaggacct gtgtagcgga gccgcaccac
     1141 aaaccgatcg ctcaagggtt tcaagggggt tgtgctgagt gacgagccac cgccagccgg
     1201 cacggtcggc agtggaatac tggactgggc ccaagcggtc gaagctgaca gtgcggctaa
     1261 cacgccagcg atcgcgactg cagatttgcg catcatttcc atccatcgcc tttcgcacag
     1321 ctagcttgcc attttcccac caggaatcaa gacctaggtt tgccgtttgc accaagaatg
     1381 actggattca gacccagaat cttgcgattc agtccactag cattcctgtc acccccctga
     1441 ctcacaggaa attaacggtt aagatcggtg cacaactgcc gagcttccca atgaactgct
     1501 gcagttgcac cagccaaaag gggagcgatc gcacgttcga tgccgtcagc cggtttgccg
     1561 gcagtccctt tctccgctga tgccatgaac gtcgctgccc cagccgccaa tttcccttca
     1621 acctttgctg ttgagttacc ggcttggctt gagcaaagtc tcagccacga ggaacagcag
     1681 agtgaggagt cgcctgacgg cagtgatcac tgtctaattg cccgcgcttt tcgcttcgcc
     1741 tattccctac atgaagggca ataccgcgcc tcgggcgagc cctacattgc ccatcctgta
     1801 gctgttgctg ggctgctccg cgatctgggt ggcagtgctg cagtgatctg tgcgggtttt
     1861 ctccacgatg ttgtcgaaga cactgaagtc acacccgagg agattgaaga gcgctttggt
     1921 gcggaagttc gtcagctcgt tgaaggcgtg accaagcttt cgaagttcaa cttctcgagc
     1981 aaaactgaac agcaggctga gaattttcgg cgcatgtttt tggccatggc ccaggacatc
     2041 cgcgtcattt tagtcaaatt ggccgatcgc ctccacaaca tgcggacgtt ggagcatctt
     2101 gcctccacaa agcagaagcg gatcgctaaa gaaacgatgg atatctttgc gccgctggcc
     2161 aatcgattgg gcattggtcg tgttaagtgg gagctggaag atctctcatt caaatatttg
     2221 gatgcagagc aatatcgctc aatccaaggc cacgtcgctg aaaaacgagc cgatcgtgag
     2281 gcgcggctag agcaatcggt acagattctg cgcgatcgct tatcacaaat tggaattgag
     2341 cccgttgatg tcagcggtcg tcccaagcac ctctacagca tctatcgcaa gatgcagatg
     2401 cagcagaagg agttccacga aatttttgct gtagcagcgc tgcggattat cgtcaatagc
     2461 aatgatgaat gctatcgagc acttgctgtt gttcacgatg cctttcgacc gattccaggt
     2521 cgctttaaag actatatcgg tctgcctaag cccaatcgct atcaatccct tcacacgacg
     2581 gtgattggcc tttcaggtcg tcccctagaa attcaaattc ggacgctgga aatgcaccga
     2641 attgctgaat acgggatcgc tgcccactgg aaatacaagg aaagcggcgg ctcagctggg
     2701 aaattttcga ccgaagacga gaagtttact tggctgcgtc agttgctgga atggcaacac
     2761 gatctcaaag atgccaagga gtatctggag aatattaaag acaacctgtt tgatgaggat
     2821 gtttacgtct tcactcccgg cggcgatgtg attgccttag cccagcgatc gacaccggtt
     2881 gactttgcct atcgcattca caccgaagtg gggaaccgtt gtgcaggtgc aaaggttaat
     2941 ggccggatag taccgctgga gactcgcctc aataacggcg atatcgtcga gattctgacc
     3001 caaaaaaatg ctcgtcctag tttggactgg ctcaattttg ttgttacttc agcggccaaa
     3061 aaccggattc gtcagtggta taaacgtagc catcgcgatg aaaacattgc acgcggccgc
     3121 gaaatgctgg agaaagagct gggcaagccc ggctttgaag cgctgctgaa atcggagccg
     3181 atgcaaaagg ttgcggagcg ctgcaactac cccagtcccg atgacctgct ggcggcgatc
     3241 ggctacggcg aaatgaccat tactttggtg gtcaatcgca tccgtgatgc tgtgcgatcg
     3301 cagcaaccgg cactgctgga aggcacagac acagcgctct cggatgcgga tctagctgcg
     3361 acgctcagcc aagccaccca gcgccacgat gcccagcgcc cggttagccg atcgccgatt
     3421 attggtgttg aagggctggt gtatcggttg gcgggctgtt gtaatcctct gcctggcgaa
     3481 tcaattctgg cagtagtttc gcggggtaac catggcattg cagtccatcg ccagagctgc
     3541 ccaaacgtgg aagggattgc gggcgatcgc ttaattccgg tgtgctggaa tccggacgaa
     3601 gtcaaagcac ctcggccaca gacctatccg gtcaatgttc agatcaccgt gctcgatcgc
     3661 gttggcgttt tacgcgatat cctgacccgc ctcagcgata acaacatcaa cgtccgcaat
     3721 gcgcaagtga aaacgacccc aggcaagcca gcgattatcg atctctgtat cgatcttgct
     3781 agtgcagatc agctgggacg gacgttttct caaatccgcc agatgagcga tgtgctgcat
     3841 atccggcggc tgtcaagcgg cagcgatgat gagctgtgat cactcagcta tccaactgat
     3901 gctgataaat accgcctaca ggcacttatt gcgctgcagg agttagcaat tgagccgcga
     3961 tcgccgctag tttttgggcc gcaccggcct caccgcgaac cgctctgagt tccgcttgca
     4021 tggccgacag tttctctggg ttctggagat agtcgagagc gatcgcggca atatcctcgg
     4081 gatagtattc gccgatcaac tcgggcacga tcgcccgctt cgcccaaata ttcggccaag
     4141 ccagcagacc cactttgcgc agcgccaagg tattaatcat gcatgcctgc aggtcgactc
     4201 tagagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct
     4261 ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     4321 cggatcaatt cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
     4381 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
     4441 tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga
     4501 acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca
     4561 ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg
     4621 tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga
     4681 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
     4741 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     4801 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     4861 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     4921 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     4981 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     5041 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     5101 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     5161 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     5221 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     5281 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     5341 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     5401 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     5461 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     5521 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     5581 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     5641 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     5701 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     5761 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     5821 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
     5881 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     5941 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     6001 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     6061 acatccgcat taaaatctag cgagggcttt actaagctga tccggtggat gaccttttga
     6121 atgaccttta atagattata ttactaatta attggggacc ctagaggtcc ccttttttat
     6181 tttaaaaatt ttttcacaaa acggtttaca agcatactag aggatcggcg gccgcggatc
     6241 cccgggtacc gagctcgaat ttcgagcttc tggagcagga agatgtcgcg ggcattagca
     6301 ccagcggtct gccaagcctc cgccagccgt tgggtccctt ccgcttgagc ttttccatct
     6361 tcgacgatac gggcggcggc cccccgcgct tccgcgatcg cccgtttaca agctgcctca
     6421 gctggggcga tcacatcggc ttgaagttgc tgctgcacct gtttgatccg ctcctgctgc
     6481 acagggagtt ctgcttggct acgagcgact tcggtagcaa tgtccgcttc agcttcggcc
     6541 accaccgctt cgcgccgcgt caacgcatcc tgaatccggc gctcggcctc ggcttgggcg
     6601 atcgctacat cgcgatcgat ccgacgcagg gccgtgatct tgtcattttc ggccgtttgg
     6661 atcgcagagg cagcctgggc atcggcttca gcaattcggg catctcgctg cagatcagcc
     6721 cgctgcttgc gtccactagc cgagagataa ccgacctcat cggaaatgtt ctggacttgc
     6781 agcgtatcga ggactagacc cagctgctca aggtcatcct ccgcctcttc cagcagactt
     6841 ttggcaaagg caattttgtc ctcgttgatc tgctccggcg tgaggctggc taaaacacca
     6901 cgcaagttgc cttcgagggt ctccttggca atttgctcga tttccttacg gtttttgcca
     6961 agcagccgct cgatcgcgtt gtggatggtc ggttcttccc cagcaatctt gatattggca
     7021 acgccttcaa cagtcagggg aatgccgccc ttggagaagg cattggaaac gcgcaactca
     7081 atgatcatgt tggtcagatc catgcggagc gctttttcca gcagaggtac ccgcaggctg
     7141 ctgccgccct tgaccaagcg atagccaact cggcggccat cactactgcg gcgactactg
     7201 ccagcaaaga tcaaaatttc actgggttgg cagatgtagt agagattgcg caggactaag
     7261 ctgccagccc cggcgactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7321 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7381 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7441 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7501 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7561 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7621 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7681 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7741 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7801 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7981 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8041 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8101 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8161 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8221 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8341 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8521 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8581 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8641 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8701 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8761 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8821 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8881 tccgctatcg ctacgtgact gggtcatggc tgcg
//
