LOCUS       sequence-196988-        8206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of kaiBC S431A T432A variant from an 
            rpaA-independent promoter in NS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: P0050::kaiBC S431A T432A NS1
FEATURES             Location/Qualifiers
     source          1..8206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..108
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             4347..5138
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7292..7880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7781..7800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8034..8051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8066..8206
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8132..8151)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      121 tcaccgaaac gcgcgaggca ggatcgatcc ggcagccggc ggagcgctgc tttcttggca
      181 agcggtcgcc agccccaacg ccagggctgc cagcccgaaa cagcggggca aggcagcttg
      241 gaagggcgat cgcagcacgg gcatggcaat gtctctctga aggaatgcag accttattcg
      301 tacagccagg gttgaatcgt gggggtccaa tcacttagct ctgctgggct aaaccagaga
      361 gcaatttcct gttgtgctgt ttcgattgca tccgagccat ggatgatgtt gcggccaata
      421 ttgacaccaa aatcaccacg gatggtgccc ggttctgccg tcagcggatt ggtagcgccg
      481 atcaacttgc gagcagccgc cacaacgcct tcgccttcca agacgatcgc cacgatcggc
      541 ccagaggtga tgaactcgac gaggccattg aagaaggggc gctcgcggtg gacagcatag
      601 tgctgttcgg ccagctcgcg actgggcttc agctgcttta ggcccaccag tttgaagcct
      661 ttttgctcaa agcggccgat gatcgtaccg accaaacccc gctgaacgcc atcgggcttg
      721 atggcaataa atgtgcgttc cacagacatc tagatagtcc tcaagacgag gcaagcattg
      781 agcttgcctt cctatggttc gggatcactg ggattcttga caagcgatcg cggtcacatc
      841 gctatctctt aggacttcgc agcgggcgag tcggattgac ccggtaggga tttcgccaga
      901 tcaatgcccg tggtttgttt cagcttctcc agcaagctag cgatttgggt agcgctgcct
      961 tccccttcgc caatcacagt gatcgactcc acgtcgatat ctggcacggt gcctgaaagc
     1021 gtgacgagca gggactcgag gccgcgctgc ctttggttgc aataacggta aatttaccgt
     1081 ctgtcccgat tgtgccagtc acgttaatat tcgctgcttt accacttcca gcagccgtta
     1141 cttggctagc attgccgcaa gttatgttaa agttacctgc actgctacca ataccaagct
     1201 gtgtacaaga cgcattggtg acgtagttag gatctagtgt cgccgcacta agggtacgaa
     1261 gagcagcacc gactgctgat ttgccttcag aggcataggc tttatctgtt tgatccaaca
     1321 ggttcggtaa tgcaactgct gccaagatgc caacgataat gataacgacc agcaactcga
     1381 caagggtaaa acctttttct tcttcacctt gttcgtcttt acgctgggca agatattgta
     1441 gataacgaag acggagggag tcagacattc aggagatcct cagcaagaga gctgttcttg
     1501 aagtaactta cccctacagc aggcttttat ttcaggttcc tgaaaatttc cgcatgagcc
     1561 ctcgtaaaac ctacattctc aagctctacg tcgccggcaa tactccaaac tcagtccgtg
     1621 ccctcaaaac gctcaagaac attctcgaag ttgaatttca aggtgtttat gctctaaagg
     1681 tgatcgatgt tctcaaaaat cctcagttgg cagaagagga taaaatccta gcgacgccaa
     1741 ccctcgccaa ggttctacca ctgcctgtcc gacggattat tggtgattta tccgaccgtg
     1801 agaaagtttt gattggcctt gatttactct acggcgaact tcaagattcc gacgacttct
     1861 aactgtcgtt aactgctttg ttggtactac ctgacttcac cctcttttaa gatgacttcc
     1921 gctgagatga ctagccctaa taataattct gagcaccaag ccatcgctaa gatgcgcacg
     1981 atgattgaag gctttgatga tattagtcat ggcggtcttc caatcgggcg atcgaccctc
     2041 gttagtggta cttcaggaac cggcaagacc cttttttcta ttcaatttct ctataacggt
     2101 attatcgagt ttgatgagcc tggggttttc gttactttcg aagaaacccc gcaagatatc
     2161 attaaaaacg cccgtagttt tggctgggat ttagccaagc tggtcgatga gggcaaacta
     2221 tttattcttg atgcttcacc cgatccagaa ggtcaagagg ttgttggcgg cttcgatctc
     2281 tctgctctga ttgagcggat taattatgca attcaaaagt atcgagcgcg gcgggtttca
     2341 attgactcgg tcacgtccgt tttccagcaa tatgatgcct cttctgtggt tcgccgcgaa
     2401 ctctttcggt tggtagctcg cctaaaacaa attggggcaa ctacggtcat gaccaccgag
     2461 cgtatcgagg aatatggccc gatcgctcgt tacggtgttg aggaatttgt ctccgataac
     2521 gtcgtgattc tccgcaacgt tttggaaggg gagcgccgtc gccgcaccct cgaaatcctc
     2581 aagctacgtg gcaccagcca catgaaaggg gaatatccgt tcacgattac ggatcatggc
     2641 atcaatatct tcccgctcgg ggcaatgcgc cttacgcagc gatcgtcgaa cgtgcgtgtt
     2701 tcatctggtg tcgtccgact cgatgaaatg tgtggtgggg gcttctttaa ggactcaatc
     2761 attctggcaa ctggcgctac aggcactggt aaaactctgt tagttagccg tttcgttgag
     2821 aatgcttgtg ctaacaaaga gcgggcgatt ctgttcgctt atgaagagtc acgagctcag
     2881 ctgctccgca atgcctattc atggggaatg gactttgagg agatggagcg ccaaaacctc
     2941 ctcaaaattg tttgcgccta tcctgaatct gcaggtcttg aagaccattt gcagattatt
     3001 aagtcggaga tcaatgactt taagccagct cgtattgcaa tcgactccct ctctgctttg
     3061 gcgcggggcg ttagcaacaa tgccttccgc caatttgtaa ttggtgtcac tggctacgcg
     3121 aaacaagaag aaatcacggg actattcaca aataccagtg atcaatttat gggagcgcat
     3181 tcgattactg actcccatat cgcagcaatt acggatacga ttatcttgct ccaatacgtc
     3241 gagattcgtg gcgaaatgtc ccgcgccatt aacgtcttca agatgcgcgg atcttggcat
     3301 gacaaagcaa tccgcgaatt catgatcagc gacaaagggc cggacatcaa ggattctttc
     3361 cggaactttg agcggattat ttcaggttcg ccaacacgga ttaccgtcga tgagaaaagc
     3421 gaactctcgc gaattgtgcg cggcgttcaa gaaaaagggc cggagagcta gtgcatgcct
     3481 gcaggtcgac tctagagctt tatgcttgta aaccgttttg tgaaaaaatt tttaaaataa
     3541 aaaaggggac ctctagggtc cccaattaat tagtaatata atctattaaa ggtcattcaa
     3601 aaggtcatcc accggatcaa ttcccctgct cgcgcaggct gggtgccaag ctctcgggta
     3661 acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga
     3721 aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca
     3781 gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat
     3841 ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc
     3901 agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac
     3961 gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt
     4021 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     4081 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     4141 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     4201 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     4261 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     4321 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     4381 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     4441 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     4501 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     4561 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     4621 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     4681 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     4741 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     4801 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     4861 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4921 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4981 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     5041 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     5101 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     5161 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     5221 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     5281 acacaaattg ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc
     5341 gaagtaatcg caacatccgc attaaaatct agcgagggct ttactaagct gatccggtgg
     5401 atgacctttt gaatgacctt taatagatta tattactaat taattgggga ccctagaggt
     5461 cccctttttt attttaaaaa ttttttcaca aaacggttta caagcatact agaggatcgg
     5521 cggccgcgga tccccgggta ccgagctcga atttcgagct tctggagcag gaagatgtcg
     5581 cgggcattag caccagcggt ctgccaagcc tccgccagcc gttgggtccc ttccgcttga
     5641 gcttttccat cttcgacgat acgggcggcg gccccccgcg cttccgcgat cgcccgttta
     5701 caagctgcct cagctggggc gatcacatcg gcttgaagtt gctgctgcac ctgtttgatc
     5761 cgctcctgct gcacagggag ttctgcttgg ctacgagcga cttcggtagc aatgtccgct
     5821 tcagcttcgg ccaccaccgc ttcgcgccgc gtcaacgcat cctgaatccg gcgctcggcc
     5881 tcggcttggg cgatcgctac atcgcgatcg atccgacgca gggccgtgat cttgtcattt
     5941 tcggccgttt ggatcgcaga ggcagcctgg gcatcggctt cagcaattcg ggcatctcgc
     6001 tgcagatcag cccgctgctt gcgtccacta gccgagagat aaccgacctc atcggaaatg
     6061 ttctggactt gcagcgtatc gaggactaga cccagctgct caaggtcatc ctccgcctct
     6121 tccagcagac ttttggcaaa ggcaattttg tcctcgttga tctgctccgg cgtgaggctg
     6181 gctaaaacac cacgcaagtt gccttcgagg gtctccttgg caatttgctc gatttcctta
     6241 cggtttttgc caagcagccg ctcgatcgcg ttgtggatgg tcggttcttc cccagcaatc
     6301 ttgatattgg caacgccttc aacagtcagg ggaatgccgc ccttggagaa ggcattggaa
     6361 acgcgcaact caatgatcat gttggtcaga tccatgcgga gcgctttttc cagcagaggt
     6421 acccgcaggc tgctgccgcc cttgaccaag cgatagccaa ctcggcggcc atcactactg
     6481 cggcgactac tgccagcaaa gatcaaaatt tcactgggtt ggcagatgta gtagagattg
     6541 cgcaggacta agctgccagc cccggcgact caccagtcac agaaaagcat cttacggatg
     6601 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6661 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6721 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6781 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     6841 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6901 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6961 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7021 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7081 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7981 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8041 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     8101 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8161 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcg
//
