LOCUS       sequence-196991-        7306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  deletes glgP_gap1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: glgP-gap1-
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(1166..1981)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1166..1981)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1889..1908
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1973..1992
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3134..4324
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3486..3505)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4963..5154
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5148..5170)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5256..5396
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5311..5330
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5411..5428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5582..6170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5662..5681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6341..7132,7133..7201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6964..6983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7202..7306)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
      121 ttatcatcga tacaaaccaa tcagcagttc gctgatgccc aaggtgcgag caaccgcgac
      181 agaattatca atcaggagct ttgccccgag gatcaggagc cccagaccag ctgcgattag
      241 caagagttgc tgccataggg gtgggcgatc gcccaatcca tattcctcgg catattccgc
      301 ctgaacctct gggtcaggtt cttctccccc ctggcgaact tggaaaacgg tgtagagaac
      361 gcccaacacc accaagacaa tgccatcgag gcggctgatc cgaccatcta gggccatccc
      421 atagaccaat cctgccgtgg caatcatgat cggcacatcg agccgaacca actgttgcgc
      481 cacctgtagg ggtgtgatta aggccgagat cccgagaata aacaacacat tgaagatgtt
      541 gctgcccacc acattcccga gcgaaatatc gttggagccc tccagaaccg cctgaatact
      601 gactgcgagc tctggagcgc tcgtcccgta ggccaccaca gtgaggccaa tgatgagtgg
      661 tgataacccc acgagtgccg ccagttttga ggctccgcgg actaaaaact cagctccggc
      721 aacgaggata actaaaccga gcagcaagaa cagtaaattg atcagcattg aggcgatggc
      781 caacatcacc ccttcatctt acggacgcgt ctgcgatcgc ccataaaaaa accccgcgtg
      841 ggcagggttt aacggtattg ctctctgact actgactggg aatggaaatc tacagcaggc
      901 caatttgaga caggatgcct tggccggtca gcaattcggt ggccaaacca accacgaacc
      961 cgatcatagc cagacggcca ttccaggttt ctgcgaatgc ggtgaaacca aacttggtat
     1021 tttgctcaga catgacaggg acctccctac ggggtggttt ggtagctgtt aagcaatgta
     1081 acgcaaaaac tcaaaatccg caacatttct tcaaaaaatc ttggttcagt tcctgccagt
     1141 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     1201 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     1261 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     1321 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     1381 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt
     1441 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     1501 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     1561 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1621 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     1681 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1741 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     1801 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1861 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     1921 catccatgtt tgaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca
     1981 taacacccct tgtattactg tttatgtaag catacagttt tattgttcat gatgatatat
     2041 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     2101 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagggtagc
     2161 cgggagaggg gaattgccct gactatagac aggggatcac ggcggtcgat cggccttaag
     2221 aggaatgaaa gaaagccgta gtgctttgca atgctcttct cagatcaggt ttgggatccc
     2281 atgcagatca aattcggctc agccgttggc tgggcaggct ggtagaaccg gcaagacgtg
     2341 attgaatgaa ggcgatcctt ttatccggcg gacccatgcc tgcgatcgcc tcgcgctttc
     2401 ttttggtact gctttgcttt ttaccgctag cagcctgtcg tagcttgggt ggcaatgaga
     2461 ctgagagcaa tagtcgcctc aaccaagtgc aggcgcgcgg taagttgctc tgcggagttg
     2521 aaggccgact gcctggcttt agcttccttg attcccaggg caattacagt ggcttggatg
     2581 tcgacatttg taaggcgatc gccgcagcac tgttcaatga tcccaaggcg atcgaatacc
     2641 gcagtctcga ttctgttgaa cggtttccag ccttagcaag tggtgaagtc gatttgctct
     2701 cgcgcaacac cacttggacc ctgagtcgcg atgccaaggg cggcaacaac cttgagtttg
     2761 cgccaaccac tttctatgac ggccaagggt tgatggtgcg gcgcaatagt ggcatccaat
     2821 cgctgcagga ttttcagggc aagtcaatct gcgtggagac gggcaccaca tcggaactca
     2881 atctggcgga cacgatgcgc gagctgggtg tccaatatca ggagattaaa tttccgaact
     2941 ccgatgccaa ctacgctgcc tatgcccaag gccgttgtga gggggtaacc tccgatcgct
     3001 ctcagctggc cgcccgtcgg acgaccttgt ccgatgccga tcagcaccaa ttgctggatg
     3061 cagtgatctc caaagagccg cttaatgcgg tagtttatca cagttaaatt gctaacgcag
     3121 tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
     3181 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
     3241 ccattccgac agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca
     3301 atttctatgc gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct
     3361 gctcgcttcg ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
     3421 gtggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     3481 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     3541 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     3601 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     3661 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
     3721 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     3781 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     3841 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     3901 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     3961 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     4021 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     4081 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     4141 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc tgatcgtcac
     4201 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     4261 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     4321 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     4381 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     4441 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     4501 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     4561 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     4621 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     4681 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     4741 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     4801 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     4861 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     4921 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     4981 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     5041 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     5101 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5161 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5221 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     5281 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5341 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     5401 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5461 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5521 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5581 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5641 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5701 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5761 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5821 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5881 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5941 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6001 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6061 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6301 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6361 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6421 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6481 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6541 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6601 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     6661 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6721 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6781 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6841 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6901 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     6961 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7021 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7081 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7141 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7201 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7261 acatatttga atgtatttag aaaaataaac aaataggggt tccgcg
//
