LOCUS       sequence-197-exp        4634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3691
FEATURES             Location/Qualifiers
     source          1..4634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1005..1033)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1781..1801
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2379..2483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2484..2552,2553..3344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2702..2721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3515..4103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4004..4023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4257..4274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4391..4412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4427..4432,4433..4450,4451..4457)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4465..4481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4470..4492
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4489..4505
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4489..4505
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcgccaa atgtaaacct
       61 gtcatttctg tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg
      121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat
      181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc
      241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt
      301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc
      361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt
      421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa
      481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc
      541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc
      601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc
      661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa
      721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg
      781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag
      841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagcaacat ccgcgagatc
      901 gtggatccta tcgattcgcg gccgctgtac acccgggtgc cctatagtga gtcgtattgg
      961 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1141 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     1201 cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga
     1261 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     1321 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
     1381 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
     1441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1501 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1561 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact
     1621 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1681 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1741 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1801 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gctttggaat
     1861 tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa
     1921 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt
     1981 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt
     2041 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct
     2101 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat
     2161 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga
     2221 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc
     2281 aaagaaatta ggcgaacgat gagaattgtc ctcgcttggg cccaaaaggc cttttttttt
     2341 tggcgccgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2461 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2521 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2581 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2641 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2701 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2761 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2821 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2881 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2941 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3001 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3061 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3121 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3181 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3241 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3301 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3361 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3421 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3661 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3901 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4201 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4261 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4321 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4381 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     4441 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     4501 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
     4561 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa
     4621 cttggaaatg aaat
//
