LOCUS       sequence-197029-        9129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of gnd using Ptrc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::gnd
FEATURES             Location/Qualifiers
     source          1..9129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        449..526
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             527..1609
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(546..565)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1841..1846,1847..1863,1864..1870)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1883..1905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             2976..4166
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3328..3347)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4805..4996
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    5098..5238
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5153..5172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5253..5270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5424..6012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5504..5523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6183..6974,6975..7043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6806..6825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7044..7148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7216..7234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(8314..9129)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8314..9129)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9037..9056
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     join(9121..9129,1..11)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
       61 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
      121 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagtgctagc
      181 tcgagcccgc gacatcttcc tgctccagaa gctcgaaatt cgagctcggt acccggggat
      241 ctgggccgcg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
      301 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      361 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
      421 cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg
      481 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
      541 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
      601 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
      661 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
      721 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
      781 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
      841 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
      901 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
      961 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     1021 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     1081 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     1141 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     1201 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     1261 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     1321 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     1381 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     1441 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     1501 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1561 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     1621 ttaatgtaag ttagcgcgaa ttgatctggt ttgacagctt atcatcgact gcacggtgca
     1681 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa
     1741 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
     1801 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc
     1861 tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatggaa
     1921 ttcatggcac tgcaacagtt cggcctcatc gggctggcag tgatgggtga gaatctcgcc
     1981 ctgaacattg agcgcaacgg tttttccttg acggtctata accgcacggc cgaaaagact
     2041 gaagcgttca tggccgatcg cgcccaaggc aagaacattg tgccggctta cagtctggaa
     2101 gactttgttg ccagcttgga acgtccgcgc cgcattttgg tgatggttaa agcgggcggc
     2161 ccggtggatg ccgtggtcga gcagctcaaa cccctgctag atcccggtga cttgatcatc
     2221 gatgggggca actcactgtt taccgacacc gagcgccgcg tcaaagatct agaagcgcta
     2281 ggtctgggtt tcatgggcat gggcgtcagt ggtggcgaag agggggcttt gaatggcccc
     2341 agtttgatgc cggggggcac ccaagcggcc tacgaagccg tggagccgat cgtgcgcagc
     2401 attgctgccc aagtcgacga tggcccctgc gttacctaca tcggtcctgg tggctctggg
     2461 cactacgtca agatggtgca caacggcatt gaatatggcg atatgcagct gatcgccgaa
     2521 gcctatgacc tcctcaaatc ggtggcaggc ctcaatgcca gtgaactgca tgatgtcttc
     2581 gccgcctgga acaaaacgcc ggaactcgac tcgttcttga ttgaaatcac ggcggatatt
     2641 ttcaccaaag tcgatgactt gggtactggt cagcccttgg tcgagctgat tttagatgcc
     2701 gctggtcaaa aaggaaccgg tcgctggacg gtggaaacgg cactggaaat tggcgttgcg
     2761 attccaacca tcattgctgc tgtcaacgcc cgcatcctgt cctcgatcaa agccgagcgt
     2821 caggcagctt ctgaaattct ttcaggaccg attaccgagc ccttcagtgg cgatcgccaa
     2881 gcctttatcg acagtgtgcg cgatgcgctc tactgctcga aaattaatgc ggtagtttat
     2941 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
     3001 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
     3061 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
     3121 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
     3181 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
     3241 tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca
     3301 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     3361 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     3421 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     3481 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     3541 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     3601 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     3661 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     3721 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     3781 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     3841 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     3901 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     3961 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     4021 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     4081 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     4141 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     4201 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     4261 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     4321 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     4381 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     4441 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     4501 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
     4561 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     4621 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
     4681 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     4741 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
     4801 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
     4861 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
     4921 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
     4981 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     5041 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     5101 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     5161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5221 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5581 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5821 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6481 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6781 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     6841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7141 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7201 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctcatgt
     7261 ttgacagctt atcatcgata agccagccta agccgtctgt cgatcccagc agcgatagag
     7321 atggaggagt tgatcgaaat agtgacgagc taattccagc tgagggaaag acttcaccga
     7381 aagctgatcg ctccggcaat ccaaaacacg aacttgatga tcttcaatat tggcaactgc
     7441 aggactgaga atgacgatcc agcgaccgat atgttgtcgt tgcgtttcca taagaaagac
     7501 agatgattgt gcgattaaag tagcgacaac aaggctaaca aatcgttacc tagggcacga
     7561 ttttgctctg aaccagagtc agtcagcaca acgctaaact tttgtagagc tttatcctcg
     7621 gctcctaaac ggttgcgatc gccatgttga ttcagcctgc tgcaacgacg tctaccccag
     7681 aggtggctct cgcaatcatc acccaaggtc aacagtggtt attgcagctg cgtgatgact
     7741 tagattggat tctctatccg gggaagtggg gcttatttgg cggccattta gaaccggatg
     7801 aaactcccct tgccgccctc tatcgcgaac tccaagaaga gattggctac tacccctccg
     7861 ccgcaacccc actctggacg gaaacagaag aaagggtcac tcgctacctc ttccatgtgc
     7921 ctttgacctg tgaactatcg actttgcggc tcggcgaagg gcaggatttc tgcctcgtcg
     7981 atgccgccgc gatcgcccaa ggtcgagttt tctcgccccg tttacagcag gaacgagcga
     8041 tcggtgcgcc ccaccagcag attttgctgc gcttccaagg ggagtattcg cacaagcttg
     8101 cccccgtcat cagggcctga ccggtgcgat cgccgtcaag aatccacccc caggcattca
     8161 atttttctct gatctgtaaa taaatccgcg atcgccgccg tgatccccgc aaagaaagtc
     8221 taaagaggtt gccagagaac cccaaattgg caggctaacc tgaggtcatt cgttgtccta
     8281 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     8341 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     8401 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     8461 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     8521 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     8581 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     8641 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     8701 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     8761 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     8821 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     8881 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     8941 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     9001 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     9061 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     9121 atggctcat
//
