LOCUS       sequence-197030-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces native promoter of glgP_gap1 with Ptrc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: Ptrc::glgP_gap1
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1381..1485)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1688..1790
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1791..2450
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1857..1876)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        2992..3069
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3070..4152
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3089..3108)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(4384..4389,4390..4406,4407..4413)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4421..4437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4426..4448
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             5516..6706
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5868..5887)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             7345..7536
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    7638..7778
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7693..7712
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7793..7810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7964..8552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8044..8063)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8723..9514,9515..9583))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9584..9688)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
      121 ttatcatcga tatgatacca aactcctccg cctgaatcgt ggcattgacc acccatttga
      181 caacgtcaaa ccaaggcgag tcattgttga gggtggctgg gctcagcggc tctttggaga
      241 tcactgcatc cagcaattgg tgctgatcgg catcggacaa ggtcgtccga cgggcggcca
      301 gctgagagcg atcggaggtt accccctcac aacggccttg ggcataggca gcgtagttgg
      361 catcggagtt cggaaattta atctcctgat attggacacc cagctcgcgc atcgtgtccg
      421 ccagattgag ttccgatgtg gtgcccgtct ccacgcagat tgacttgccc tgaaaatcct
      481 gcagcgattg gatgccacta ttgcgccgca ccatcaaccc ttggccgtca tagaaagtgg
      541 ttggcgcaaa ctcaaggttg ttgccgccct tggcatcgcg actcagggtc caagtggtgt
      601 tgcgcgagag caaatcgact tcaccacttg ctaaggctgg aaaccgttca acagaatcga
      661 gactgcggta ttcgatcgcc ttgggatcat tgaacagtgc tgcggcgatc gccttacaaa
      721 tgtcgacatc caagccactg taattgccct gggaatcaag gaagctaaag ccaggcagtc
      781 ggccttcaac tccgcagagc aacttaccgc gcgcctgcac ttggttgagg cgactattgc
      841 tctcagtctc attgccaccc aagctacgac aggctgctag cggtaaaaag caaagcagta
      901 ccaaaagaaa gcgcgaggcg atcgcaggca tgggtccgcc ggataaaagg atcgccttca
      961 ttcaatcacg tcttgccggt tctaccagcc tgcccagcca acggctgagc cgaatttgat
     1021 ctgcatggga tcccaaacct gatctgagaa gagcattgca aagcactacg gctttctttc
     1081 attcctctta aggccgatcg accgccgtga tcccctgtct atagtcaggg cagtcacacg
     1141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1501 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1561 tatcacgagg ccctttcgtc ttcgaataaa tacctgtgac ggaagatcac ttcgcagaat
     1621 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
     1681 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     1741 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     1801 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     1861 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     1921 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     1981 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     2041 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     2101 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     2161 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     2221 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     2281 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     2341 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     2401 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     2461 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
     2521 tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta
     2581 aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac
     2641 cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat
     2701 tgacgatatg atcgactgct agctcgagcc cgcgacatct tcctgctcca gaagctcgaa
     2761 attcgagctc ggtacccggg gatctgggcc gcgggtcatg gctgcgcccc gacacccgcc
     2821 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2881 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2941 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc
     3001 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     3061 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     3121 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     3181 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     3241 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     3301 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     3361 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     3421 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     3481 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     3541 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     3601 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     3661 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     3721 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     3781 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     3841 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     3901 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     3961 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     4021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     4081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4141 agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgatct ggtttgacag
     4201 cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt
     4261 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg
     4321 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag
     4381 ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     4441 cacacaggaa acagaccatg gaattcatgt ccaaaattcg tgttggaatt aacggctttg
     4501 gccgtattgg tcgtctctgc ttacgggctg gatttgatga tccgcaactc gattttgttg
     4561 cgattaacga cctcgttccg gccaaaaatc ttgcctatct tcttcactac gactccaccc
     4621 acggccgcct acaacaaaca gtgcgggcag aagaagatcg catttgtgcc ggcgatcgct
     4681 gcattcaatg tttctccgaa actgatccgg cagctattcc ttggggggag ttaggggttg
     4741 atgtggtgat tgaggctacg gggcgcttca ccgattacgc tgccgccagt cgccacctgg
     4801 aagcaggcgc tcaacgggta gtgatttcgg ccccgacctc ggagaaagat cccgatcgcg
     4861 ttcccacctt cgtctacggc gtcaatcatc agaactacga cccgcaacag catcgcattc
     4921 tctccaacgc cagctgcacc accaactgcc tggctccgat cgccaaggtg cttcaagagc
     4981 actgcgggat tgaagacggt ctgatgacta ccatccatgc ggtcaccgcc acccaaccaa
     5041 ctgtcgatgg acccagccgt aaagatatgc gcggcgggcg cagtgcggct caaaacatca
     5101 ttccggccag tacgggcgct gccaaagccg tgggcttggt gctgcccgaa ctccagggtc
     5161 acctgaccgg tatggcgttg cgggttccga cggcggatgt ctcggtggtg gatctgacct
     5221 tccgtagccg ccaatccacc acctatgccg ccatctgtga ggtgatgcaa aaggcggcca
     5281 acacccaact ccaaggcatc ctgggctata ccgaggatga cctcgtttcg agtgacttta
     5341 ttggcgatgc tcggtccagc atttttgatg ccacggcagg catggctctc aacgatcgct
     5401 tcttcaaagt gatcgcttgg tacgacaacg aatggggata cagcaaccgc atgttggatc
     5461 tgattaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     5521 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     5581 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     5641 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     5701 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     5761 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg
     5821 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     5881 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     5941 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     6001 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     6061 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     6121 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     6181 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     6241 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     6301 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     6361 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     6421 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     6481 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     6541 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     6601 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     6661 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     6721 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     6781 gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc
     6841 cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     6901 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     6961 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     7021 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     7081 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
     7141 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
     7201 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
     7261 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
     7321 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
     7381 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
     7441 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
     7501 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7561 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7621 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     7681 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7741 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     7801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8401 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9001 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     9061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     9361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9661 agaaaaataa acaaataggg gttccgcg
//
