LOCUS       sequence-197040-       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington 
            LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
  TITLE     Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
  JOURNAL   Nature. 2017 Sep 20. doi: 10.1038/nature24268.
  PUBMED    28931002
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Sep 20. doi: 10.1038/nature24268."
COMMENT     Sequence Label: pJSC131 - Bacterial expression plasmid for
            SpC...
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     946..965
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1049..1068
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1049..1067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1156..1178
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1198..1227
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1237..2337
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2311..2334
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2392..2412
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2419..6525
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTAFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGALSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     ALIHDDCLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKCKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(6651..6669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6665..6712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(7078..7083,7084..7100,7101..7106))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7133..7151)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7219..7323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7324..7392,7393..8184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7542..7561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8355..8943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8844..8863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9097..9114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9129..9268
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9194..9213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9354..9376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9370..9561)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc
       61 gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc
      121 ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac
      181 aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc
      241 ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag
      301 cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
      361 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
      421 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat
      481 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
      541 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
      601 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
      661 gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
      721 tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag
      781 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
      841 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
      901 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      961 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     1021 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg
     1081 tttccctcta gtgccggctc cggagagctc tttaattaag cggccgccct gcaggactcg
     1141 agttctagaa ataattttgt ttaactttaa gaaggagata tacatatgaa atcttctcac
     1201 catcaccatc accatcacca tcaccatggt tcttctatga aaatcgaaga aggtaaactg
     1261 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
     1321 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
     1381 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt
     1441 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
     1501 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
     1561 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa
     1621 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
     1681 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat
     1741 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc
     1801 gcaaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac
     1861 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac
     1921 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg
     1981 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac
     2041 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat
     2101 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac
     2161 gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc ccagaaaggt
     2221 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
     2281 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat
     2341 tcgagctcga acaacaacaa caataacaat aacaacaacc tcgggatcga ggaaaacctg
     2401 tacttccaat ccaatgcaat ggataagaaa tactcaatag gcttagatat cggcacaaat
     2461 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
     2521 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
     2581 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
     2641 cggaagaatc gtatttctta tctacaggag attttttcaa atgagatggc gaaagtagat
     2701 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
     2761 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
     2821 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
     2881 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta
     2941 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa
     3001 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca
     3061 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa
     3121 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca
     3181 aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat
     3241 ttagataatt tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag
     3301 aatttatcag atgctatttt actttcagat atcctaagag taaatactga aataactaag
     3361 gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt
     3421 ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa
     3481 tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa
     3541 tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat
     3601 cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt
     3661 cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa
     3721 gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta tgttggtcca
     3781 ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc
     3841 ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc
     3901 atgacagcgt ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt
     3961 tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg
     4021 cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa
     4081 acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatct
     4141 tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac
     4201 catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat
     4261 atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa
     4321 agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc
     4381 cgttatactg gttggggagc gttgtctcga aaattgatta atggtattag ggataagcaa
     4441 tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg
     4501 gccctgatcc atgatgattg tttgacattt aaagaagaca ttcaaaaagc acaagtgtct
     4561 ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa
     4621 aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat
     4681 aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag
     4741 aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag
     4801 attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat
     4861 tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt
     4921 gattatgatg tcgatcacat tgttccacaa agtttcctta aagacgattc aatagacaat
     4981 aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa
     5041 gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa
     5101 cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct
     5161 ggttttatca aacgccaatt ggttgaaact cgcgccatca ctaagcatgt ggcacaaatt
     5221 ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa
     5281 gtgattacct taaaatgtaa attagtttct gacttccgaa aagatttcca attctataaa
     5341 gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga
     5401 actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa
     5461 gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca
     5521 aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat
     5581 ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg
     5641 gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt
     5701 gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt accaaaaaga
     5761 aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt
     5821 gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg
     5881 aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag aagttccttt
     5941 gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta
     6001 atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg
     6061 gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat
     6121 tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa
     6181 aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt
     6241 gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat
     6301 aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg
     6361 ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa
     6421 cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt
     6481 ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actaataaca ttggaagtgg
     6541 ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc
     6601 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     6661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     6721 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     6781 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     6841 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     6901 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     6961 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     7021 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     7081 aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7141 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataacattg aaaaaggaag
     7321 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7381 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7441 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7501 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7561 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7621 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7681 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7741 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7801 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7861 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7921 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7981 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8041 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8101 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8161 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8221 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8281 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8341 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8401 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8461 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8521 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8581 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8641 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8701 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8761 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8821 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8881 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8941 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9001 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9061 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9121 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
     9181 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     9241 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     9301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     9361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     9421 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     9481 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     9541 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     9601 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     9661 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     9721 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     9781 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     9841 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     9901 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     9961 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
    10021 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tgg
//
