LOCUS       sequence-197087-       10247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA and mouse E-cadherin -mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, 
            Hamilton NA, Neufeld Z, Yap AS
  TITLE     Cortical F-actin stabilization generates apical-lateral patterns of 
            junctional contractility that integrate cells into epithelia.
  JOURNAL   Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 
            2014 Jan 12.
  PUBMED    24413434
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2900"; 
            journalName: "Nat Cell Biol"; date: "2014-02"; volume: "16"; issue: 
            "2"; pages: "167-78"
COMMENT     Sequence Label: pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry
FEATURES             Location/Qualifiers
     source          1..10247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        28..407
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     562..582
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             625..805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    852..977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1474..1707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1892..1936
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2234..2351
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2406..2718
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2637..2657
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2775..2791
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2833..2866)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6097..6106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6103..6813
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6248..6266)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6516..6535
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6839..6872)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6928..7514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6981..7001)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7399..7410)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7517..7533)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8043..8223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8285..8873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8365..8384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9044..9835,9836..9904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9667..9686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9905..10009)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10084..10103)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      781 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      841 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      901 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      961 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1021 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1081 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1141 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1201 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1261 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1321 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1381 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1441 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1501 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1561 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1621 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1681 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1741 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1801 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1861 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1921 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1981 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2041 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2101 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2161 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2221 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2281 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2341 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgcgct
     2401 ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga
     2461 gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag
     2521 aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac
     2581 atgataggct tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc
     2641 acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc
     2701 ttggagaaaa gccttgtttg ggttaagcac aacagcaatt caagagattg ctgttgtgct
     2761 taaccctttt ttctcgaggt cgacggtatc gataagctcg cttcacgaga ttccagcagg
     2821 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccaa
     2881 gcttaagcgg ccgctgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     2941 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
     3001 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3061 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     3121 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     3181 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     3241 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
     3301 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg
     3361 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcatcg aattctgcag
     3421 tcgacggtac cgcggatggg agcccggtgc cgcagctttt ccgcgctcct gctcctgctg
     3481 caggtctcct catggctttg ccaggagctg gagcctgagt cctgcagtcc cggcttcagt
     3541 tccgaggtct acaccttccc ggtgccggag aggcacctgg agagaggcca tgtcctgggc
     3601 agagtgagat ttgaaggatg caccggccgg ccaaggacag ccttcttttc ggaagactcc
     3661 cgattcaaag tggcgacaga cggcaccatc acagtgaagc ggcatctaaa gctccacaag
     3721 ctggagacca gtttcctcgt ccgcgcccgg gactccagtc atagggagct gtctaccaaa
     3781 gtgacgctga agtccatggg gcaccaccat caccggcacc accaccgcga ccctgcctct
     3841 gaatccaacc cagagctgct catgtttccc agcgtgtacc caggtctcag aagacagaaa
     3901 cgagactggg tcatccctcc catcagctgc cccgaaaatg aaaagggtga attcccaaag
     3961 aacctggttc agatcaaatc caacagggac aaagaaacaa aggttttcta cagcatcacc
     4021 ggccaaggag ctgacaaacc ccccgttggc gttttcatca ttgagaggga gacaggctgg
     4081 ctgaaagtga cacagcctct ggatagagaa gccattgcca agtacatcct ctattctcat
     4141 gccgtgtcat caaatgggga agcggtggag gatcccatgg agatagtgat cacagtgaca
     4201 gatcagaatg acaacaggcc agagtttacc caggaggtgt ttgagggatc cgttgcagaa
     4261 ggcgctgttc caggaacctc cgtgatgaag gtctcagcca ccgatgcaga cgatgacgtc
     4321 aacacctaca acgctgccat cgcctacacc atcgtcagcc aggatcctga gctgcctcac
     4381 aaaaacatgt tcactgtcaa tagggacacc ggggtcatca gtgtgctcac ctctgggctg
     4441 gaccgagaga gttaccctac atacactctg gtggttcagg ctgctgacct tcaaggcgaa
     4501 ggcttgagca caacagccaa ggctgtgatc actgtcaagg atattaatga caacgctcct
     4561 gtcttcaacc cgagcacgta tcagggtcaa gtgcctgaga atgaggtcaa tgcccggatc
     4621 gccacactca aagtgaccga tgatgatgcc cccaacactc cggcgtggaa agctgtgtac
     4681 accgtagtca acgatcctga ccagcagttc gttgtcgtca cagaccccac gaccaatgat
     4741 ggcattttga aaacagccaa gggcttggat tttgaggcca agcagcaata catccttcat
     4801 gtgagagtgg agaacgagga accctttgag gggtctcttg tcccttccac agccactgtc
     4861 actgtggacg tggtagacgt gaatgaagcc cccatcttta tgcctgcgga gaggagagtc
     4921 gaagtgcccg aagactttgg tgtgggtcag gaaatcacat cttataccgc tcgagagccg
     4981 gacacgttca tggatcagaa gatcacgtat cggatttgga gggacactgc caactggctg
     5041 gagattaacc cagagactgg tgccattttc acgcgcgctg agatggacag agaagacgct
     5101 gagcatgtga agaacagcac atatgtagct ctcatcatcg ccacagatga tggttcaccc
     5161 attgccactg gcacgggcac tcttctcctg gtcctgttag acgtcaatga caacgctccc
     5221 atcccagaac ctcgaaacat gcagttctgc cagaggaacc cacagcctca tatcatcacc
     5281 atcttggatc cagaccttcc ccccaacacg tcccccttta ctgctgagct aacccatggg
     5341 gccagcgtca actggaccat tgagtataat gacgcagctc aagaatctct cattttgcaa
     5401 ccaagaaagg acttagagat tggcgaatac aaaatccatc tcaagctcgc ggataaccag
     5461 aacaaagacc aggtgaccac gttggacgtc catgtgtgtg actgtgaagg gacggtcaac
     5521 aactgcatga aggcgggaat cgtggcagca ggattgcaag ttcctgccat cctcggaatc
     5581 cttggaggga tcctcgccct gctgattctg atcctgctgc tcctactgtt tctacggagg
     5641 agaacggtgg tcaaagagcc cctgctgcca ccagatgatg atacccggga caatgtgtat
     5701 tactatgatg aagaaggagg tggagaagaa gaccaggact ttgatttgag ccagctgcac
     5761 aggggcctgg atgcccgacc ggaagtgact cgaaatgatg tggctcccac cctcatgagc
     5821 gtgccccagt atcgtccccg tcctgccaat cctgatgaaa ttggaaactt catcgatgaa
     5881 aacctgaagg cagccgacag cgaccccacg gcaccccctt acgactctct gttggtgttc
     5941 gattacgagg gcagtggttc tgaagccgct agcctgagct cactgaactc ctctgagtcg
     6001 gatcaggacc aggactacga ttatctgaac gagtggggca accgattcaa gaagctggcg
     6061 gacatgtacg gcggtggcga ggacgacaaa ccggtcgcca ccatggtgag caagggcgag
     6121 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     6181 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6241 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     6301 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     6361 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6421 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     6481 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     6541 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6601 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6661 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     6721 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     6781 tccaccggcg gcatggacga gctgtacaag taatgcaggc aattcgtcga gggacctaat
     6841 aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag
     6901 gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     6961 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7021 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7081 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7141 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7201 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7261 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7321 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7381 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg gcctgctgcc
     7441 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7501 ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa
     7561 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     7621 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     7681 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7741 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     7801 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     7861 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     7921 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     7981 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     8041 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8101 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8161 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8221 gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8701 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9001 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9061 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9661 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10021 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
    10081 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    10141 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    10201 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttc
//
