LOCUS       sequence-197088-        6376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with IFI27 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_I...
FEATURES             Location/Qualifiers
     source          1..6376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1012..1133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1023..1042)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1077..1096
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3774..3791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(3949..4017,4018..4809)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(4999..5587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5079..5098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      5686..5695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5692..6376,1..968)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
       61 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
      121 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
      181 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
      241 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
      301 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
      361 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
      421 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
      481 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
      541 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
      601 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
      661 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
      721 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
      781 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
      841 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
      901 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
      961 ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     1021 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1081 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1141 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1201 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     1261 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactagcag ttttggattg
     1321 ggtttgtgag acactgtgcc aggcaagtgt ttttgcataa ctggctcgct ctccctgtgc
     1381 ttctagctgt ccttgtgtgg cccatgtgat aagctgcagt ttggaaacat ttcttgtgat
     1441 agttcctgtt agcaggcaaa cgggagagcc tcatcttgcg tgagagccct cccattaaag
     1501 ccttgctggc tctgtctgct gaggttttac ccaagtgact ccattttgaa tcttacaact
     1561 cgcacactac tcatgtggaa gatttaaatg tacattccag gacctggtgc tttctcttcc
     1621 gcctgttctc aaagcagctg cagagtgagc ctgactgtgt catttctcag cttcatctcc
     1681 ctttccccaa aaaggtatag atgataatcc cctgcaatgg cttcaggaca caagatcaag
     1741 tccttgcagt gcttttgtgg cctcacaggg cctggatccc tgttaaactc tgagttcacc
     1801 tcctgcctct ctcccctgcc cacctgctga ggccacactg gcccccttgc tgctcctgcc
     1861 acacgggctc tgccactgct gtggcctcca cctggaatga ctgccccccc ataattctgc
     1921 ctaggtcact gcttcacctc cttttgcttt agcgcttcat caaataacat tctatacatt
     1981 tagttattta ttatgtttag tttgccccca ttgctggtaa actctgtgag gatggatatt
     2041 ttggtctgtt ttgtttacag tcctgtccca gtgcctagtc agtatctagc acagtgggcc
     2101 ttcagttgag actttttttg aaaaacggaa catctgccta tcgcaaggac tactattatt
     2161 ctgaaaatca ccttcttcat tagaaagtaa tatttatcat tttattatag aactttgatc
     2221 ttacttcttg tgacttcatt ctgcgtagag cacactccca tccttgaatt aaatgacaaa
     2281 gcattttata ttaactgaca atgactgatg ccatgggcaa atcctatttc tgtaaataac
     2341 tgaattttcc tctggactgc gcatgagggg agaaagatgt ctgcagtttc ggtttcctgg
     2401 aaaatgaaac ctatctcatt tgttgcctgt gtcaaggggc agtgcttcag tcggggtgga
     2461 gctgcttaaa aggcctggga tcacaccctt tgggaacaca tccaagctta agacggtgag
     2521 gtcagcttca cattctcagg aactctcctt ctttgggtaa gactgggagg gtgggcagga
     2581 gctacccttc ccgtggcccc ggaccttggg tgggctgtgg gctcagggag cggaggggag
     2641 gccttaagca tccactctct gcccggtgtt tttgttctca tcagggagcc tcagatggga
     2701 agggactcga gccccacctg tccctggact ctggaatgta aggacaatta gctttgtcct
     2761 tgcccaggtt ttcctcaggc cttgagggcg gcttggggta aagtgggcaa ggggaagaga
     2821 taagtgttag gaggaaccaa gtcgaagcca ttgcaagtcc atgcctgggc ggggtgttgc
     2881 ttccctatgg aaagcttctg acctggagca aatcactgag ccagatcgcg ctccctcatc
     2941 tgtaacatgc ggaggaggag ggtcccatct ttttcacgtt agtgaggaga ttacataaga
     3001 gcaggcacct cgcctgctgt atatgcctta aaaatgcgat tggttctgat ttcttagttt
     3061 tggtgctttt tcaattgctc cgtggagaga taagggagtc ccggaagtgt ctaagacatt
     3121 ggcgctggga ctttcaggag aaagaaagca gcccccctgg ggaaataaag tccctcaggg
     3181 ccctgaccta acacaggtcc tttggtagcc ccaactcccc aacatccctc cctccctcac
     3241 cccaggatcc tttcaaggcc tgctgctcca caagctcaga gcagcctccc tagccccctg
     3301 gagcccgtca catttttcag gacagtggga agcaagtcag gttgtgtgcc catcccgtcc
     3361 tcagagctcc atcccttcgg caggtctggc tgaagttgag gatctcttac tctctaggcc
     3421 acggaattaa cccgagcagg catggaggcc tctgctctca cctcatcagc agtgaccagt
     3481 gtggccaaag tggtcagggt ggcctctggc tctgccgtag ttttgcccct gggtgagtgt
     3541 tcctgggagg ggctggtgct gggggcgagg aggctgctgg gaagggcggg ggtcctgtcc
     3601 agggacccgt gggagagaaa atgggggaca cccgcagcct tgctgccctg tcctgtcttc
     3661 tcacagcagg cgtccaccct aactttccat ctgggagggt cgtcgccgca cttatgactg
     3721 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3781 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3841 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtc ttgagagacg
     3901 tactagtaac cctgataaat gcttcaataa tattgaaaaa ggacgagtat gagcatccaa
     3961 cattttcgtg tcgcactcat tcccttcttt gcggcatttt gcttgcctgt ttttgcacac
     4021 cccgaaacgc tggtgaaagt aaaagatgct gaagatcaac tgggtgcaag agtgggctat
     4081 atcgaactgg atctcaatag cggcaagatc cttgagtctt tccgccccga agaacgattc
     4141 ccgatgatga gcactttcaa agtactgcta tgtggcgcgg tgttgtcccg tatagacgcc
     4201 gggcaagagc agcttggtcg ccgtatacac tactcacaaa acgacttggt tgagtactcg
     4261 ccggtcacgg aaaagcatct tacggatggc atgacggtaa gagaattgtg tagtgctgcc
     4321 attaccatga gcgacaacac cgcggccaac ttacttctga caacgatcgg aggccctaag
     4381 gagctgactg catttcttca taatatgggt gatcatgtga cccggcttga ccgctgggaa
     4441 ccagagttga acgaagccat accgaacgac gagcgtgata ccacgatgcc agtagcaatg
     4501 gccacaactc ttcggaaact actcactggc gaacttctta ctctagcatc acgacagcag
     4561 ctcatagact ggatggaggc ggacaaagta gcaggaccac ttcttcgctc ggccctccct
     4621 gctggctggt tcattgctga caaatcgggg gccggtgaac gcggctctcg cggcatcatt
     4681 gctgcgctgg ggcctgatgg taagccctca cgaatcgtag tgatctacac gacggggagt
     4741 caggccacta tggacgaacg aaataggcag atcgctgaga tcggtgcctc actgatcaag
     4801 cactggtaac cactgcagtg gtttagcatt tgcggccgct gtcagaccaa gtttactcat
     4861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4921 tttttgataa tctcatgaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5161 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5401 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5641 acgttaaggg attttgaagc ttggcaatcc ggtactgttg gtaaagccac catggaagat
     5701 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
     5761 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
     5821 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
     5881 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
     5941 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
     6001 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
     6061 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
     6121 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
     6181 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
     6241 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
     6301 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     6361 gaccccatct tcggca
//
