LOCUS       sequence-197089-        6007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with ISG15 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_I...
FEATURES             Location/Qualifiers
     source          1..6007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1836..1853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2011..2079,2080..2871)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3061..3649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3141..3160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3748..3757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3754..5406
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5450..5571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5461..5480)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5515..5534
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctctactaaa aatacaaaaa attagccggg cctggtggtg cacccctgtg gtcccagcta
       61 cgtgggaggc tgaggtgaga ggatcgcttt agcctggcag gcggaggttg caatgagatg
      121 agatcgtgcc tctgcactcc agcctgggcg acagagtgag agaccctgtc tcaaaaacac
      181 aaaaacaaca acaacaaaaa aacaccaatc tgagcaaata ctgccctaaa ccgagtgttg
      241 ttatctctgg gtagtttgga gttcttgttt ctcaattaac catggggatg ttttccaagt
      301 ttactaattt tgcaagttgg taaatggaaa atgaaaccat tagtccatgt gatgacagct
      361 ttagtgcatc ctgtgaagga tctggaatgc gcgatattta ggtgtttcca gggtgttggg
      421 tgggggtggg gatgccgtcc gctgtccgga gtccccacca cttttgcttt tccctgtctt
      481 tcggtcattc ggttttgttt cttccgctca ctctggggca tgcctcggga aagggaaacc
      541 gaaactgaag ccaaatttgg ccaccagcgc aggctcggcg gcacgccccc tgacgtgtgt
      601 gcctcaggct taataatagg gccggtgctg cctgcggaag ccggcggctg agaggcagcg
      661 aactcatctt tgccagtaca ggagctcgtg ccgtggccca cagcccacag cccacagcca
      721 tggtaaggca gatgtcacag gtggggggag gtgggctctg tgccagccaa ttttcgtctc
      781 cctcccccag ccaaggtctc ccaggggtgc agggagagcg gagctgctca gagcttggcc
      841 aggttctaag tgtgctcctg aaagcaggtc acccctgaga tcctcagggt ggggcacaga
      901 ggggcaccct agcaggtaaa gggaggccac gggatggcgg tgggcagctg gccttctagt
      961 aacgagccct cagtgccttc tgtgcctggg gtccctgccg acgggatgta gaggacagac
     1021 aggagggagc actgtccctg ggtacaggag ctcgccctgc agccagtgcc ttgtgtgtgg
     1081 tgggcctggg gctggcgccg cagtctctga acctgtgtga cgcctgcagg gctgggacct
     1141 gacggtgaag atgctggcgg gcaacgaatt ccaggtgtcc ctgagcagct ccatgtcggt
     1201 gtcagagctg aaggcgcaga tcacccagaa gatcggcgtg cacgccttcc agcagcgtct
     1261 ggctgtccac ccgagcggtg tggcgctgca ggacagggtc ccccttgcca gccagggcct
     1321 gggccccggc agcacggtcc tgctggtggt ggacaaatgc gacgaacctc tgaacatcct
     1381 ggtgaggaat aacaagggcc gcagcagcac ctacgaggtg cggctgacgc agaccgtggc
     1441 ccacctgaag cagcaagtga gcgggctgga gggtgtgcag gacgacctgt tctggctgac
     1501 cttcgagggg aagcccctgg aggaccagct cccgctgggg gagtacggcc tcaagcccct
     1561 gagcaccgtg ttcatgaatc tgcgcctgcg gggaggcggc acagagcctg gcgggcggag
     1621 ctaagggcct ccaccagcat ccgagcagga tcaagggccg gaaataaagg ctgttgtaaa
     1681 gagaaatggc tgcctctgtg tctgtgcttg cccctcctcc agcgtccgcc tcagacccct
     1741 ggtgatgggg cgggagaagt ctcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     1801 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1921 gaatcagggg ataacgcagg aaagaacatg tcttgagaga cgtactagta accctgataa
     1981 atgcttcaat aatattgaaa aaggacgagt atgagcatcc aacattttcg tgtcgcactc
     2041 attcccttct ttgcggcatt ttgcttgcct gtttttgcac accccgaaac gctggtgaaa
     2101 gtaaaagatg ctgaagatca actgggtgca agagtgggct atatcgaact ggatctcaat
     2161 agcggcaaga tccttgagtc tttccgcccc gaagaacgat tcccgatgat gagcactttc
     2221 aaagtactgc tatgtggcgc ggtgttgtcc cgtatagacg ccgggcaaga gcagcttggt
     2281 cgccgtatac actactcaca aaacgacttg gttgagtact cgccggtcac ggaaaagcat
     2341 cttacggatg gcatgacggt aagagaattg tgtagtgctg ccattaccat gagcgacaac
     2401 accgcggcca acttacttct gacaacgatc ggaggcccta aggagctgac tgcatttctt
     2461 cataatatgg gtgatcatgt gacccggctt gaccgctggg aaccagagtt gaacgaagcc
     2521 ataccgaacg acgagcgtga taccacgatg ccagtagcaa tggccacaac tcttcggaaa
     2581 ctactcactg gcgaacttct tactctagca tcacgacagc agctcataga ctggatggag
     2641 gcggacaaag tagcaggacc acttcttcgc tcggccctcc ctgctggctg gttcattgct
     2701 gacaaatcgg gggccggtga acgcggctct cgcggcatca ttgctgcgct ggggcctgat
     2761 ggtaagccct cacgaatcgt agtgatctac acgacgggga gtcaggccac tatggacgaa
     2821 cgaaataggc agatcgctga gatcggtgcc tcactgatca agcactggta accactgcag
     2881 tggtttagca tttgcggccg ctgtcagacc aagtttactc atatatactt tagattgatt
     2941 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttgaa
     3721 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
     3781 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     3841 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     3901 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     3961 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     4021 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     4081 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     4141 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     4201 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     4261 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     4321 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     4381 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     4441 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     4501 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     4561 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     4621 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     4681 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     4741 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     4801 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     4861 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     4921 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     4981 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     5041 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     5101 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     5161 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     5221 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     5281 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     5341 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     5401 gtgtaataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     5461 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5521 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5581 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     5641 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     5701 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcctgg atgctatgcc
     5761 ctcatcagat agataatttg caaatatttc ttcccattct atggattgtc ttttcacttt
     5821 ctcaatagtg tcccagagtt catttttgta gaaaataaaa gataggtctc ttttattaaa
     5881 aaacaatctg aggctccggg tgcagtggct cacgcctgta atcccagcag tttcagaggc
     5941 cgaggcaggt ggatcacttg agcccaggag ttcgagatca gcctgggcga catggcgaga
     6001 cccccat
//
