LOCUS       sequence-197090-        6158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with MX1 enhancer inserted downstream 
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_MX1
FEATURES             Location/Qualifiers
     source          1..6158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1891..1908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2066..2134,2135..2926)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3116..3704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3196..3215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3803..3812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3809..5461
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5505..5626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5516..5535)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5570..5589
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggccacctgt acacaatgcc tgaacccagg tcctgcagcc cccagcttgg ctctccaaag
       61 ctcaccagta tcaaaggatt tcagcttctg ctcctctccg cagggctctc tggtgggccc
      121 cagacagaga tgaaggaaaa gtctggcctt gaccttgagg accaaaagcg accacgtcag
      181 acgccagcag tctcgtgccc agttgccaag ggatcacctg aggaaggccc ttaaaataca
      241 ggtggtaatg caggggtctt gggatggccc cggaccgttc atttctaata atttcccagg
      301 tgaagccaat gctgctgttc caggaaccac cctttgagaa gcacgggtct ataccacacg
      361 cacagaagag gaaacgaaac gaggcaaagg aaaccagtcc ctttactcgc gcccaggcag
      421 gacaaagcgg gcaagggtgg gggcgggggc gcgggcgcgg gcgcgggcag gcggacggac
      481 ttgcaggaaa tgcagccggg aggatgggca ggttgtggga tgctccgccg ttccgcgacc
      541 acccgcctcc ccggcccagc agaaccgggt ccaggctggg ggaggtgcta gcggtgtatg
      601 tgagtaacac tgccccctcg tcgtggcacc gcccccttct tccctgcccc cttccccgcc
      661 ccctggattc tgaagtctga agtcatgcaa tttgactttt cctttctgga aaccagcgag
      721 cagaaatgaa accgaaactg aattgtccgg gaaattcgcg gtgggggcgg agagcgcagg
      781 gagaagtaag cccagtgcag gatcctgagg cccgtgtttg caggaccagg gccggccttc
      841 cgattcccca ttcattccag aagcaccgaa ccacgctgtg cccggatccc aagtgcagcg
      901 gcacccagcg tgggcctggg gttgccggtt gacccggtcc tcagcctggt agcagaggcc
      961 aggccagtgc cacaaggtca ggctccactg ggccctgccc gcccaggagc ctgtggtgtc
     1021 aggcacctga aggggtgggt tgcccctcca cacctgtggg tgtttctcgt taggtggaag
     1081 gagagacttg gaaaagaaaa agacacagag acaaagtata gagaaagaaa taaggggacc
     1141 cagggaacca gcgttcagca tatggaggat cccgccagcc tctgagttcc cttagtattt
     1201 attgatcatt tgtgggtgtt tctccgagag ggggatgtgt cagggtcaca agacaatagt
     1261 ggggagagag tcagcagaca aacacgtgaa caaaggtctt tgcatcatag acagggtaaa
     1321 gaatcaagtg ctgtgctttt agatatgcat acacataaac gtctcaatgc tttacaaagc
     1381 agtattgctg cctgcatgtc ccacctccag ccttaaagtg gtttttccct atctcagtag
     1441 atggaacgta caatcgggtt ttataccgag acattccatt gcccagggac gggcaggaga
     1501 cagatgcctt cctcttgtct caactgcaag aggcatgcct tcctcttata ttaatcctcc
     1561 tcagcacaga ccctttatgg gtgtcggact gggggacggt caggtctttc ccttcccacg
     1621 aggccgtatt tcagactatc acatggggag aaaccttgga caatacctgg ctttcctagg
     1681 cagaggtccc tgcggccttc cgcagtgttt gtgtccctgg gtacttgaga ttagggcgtg
     1741 gtgatgactc ttaaggagca tgctgccttc aagcatctgt ttaacaaagc acatcttgca
     1801 ccgcccttaa tccatttcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     1861 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1981 aggggataac gcaggaaaga acatgtcttg agagacgtac tagtaaccct gataaatgct
     2041 tcaataatat tgaaaaagga cgagtatgag catccaacat tttcgtgtcg cactcattcc
     2101 cttctttgcg gcattttgct tgcctgtttt tgcacacccc gaaacgctgg tgaaagtaaa
     2161 agatgctgaa gatcaactgg gtgcaagagt gggctatatc gaactggatc tcaatagcgg
     2221 caagatcctt gagtctttcc gccccgaaga acgattcccg atgatgagca ctttcaaagt
     2281 actgctatgt ggcgcggtgt tgtcccgtat agacgccggg caagagcagc ttggtcgccg
     2341 tatacactac tcacaaaacg acttggttga gtactcgccg gtcacggaaa agcatcttac
     2401 ggatggcatg acggtaagag aattgtgtag tgctgccatt accatgagcg acaacaccgc
     2461 ggccaactta cttctgacaa cgatcggagg ccctaaggag ctgactgcat ttcttcataa
     2521 tatgggtgat catgtgaccc ggcttgaccg ctgggaacca gagttgaacg aagccatacc
     2581 gaacgacgag cgtgatacca cgatgccagt agcaatggcc acaactcttc ggaaactact
     2641 cactggcgaa cttcttactc tagcatcacg acagcagctc atagactgga tggaggcgga
     2701 caaagtagca ggaccacttc ttcgctcggc cctccctgct ggctggttca ttgctgacaa
     2761 atcgggggcc ggtgaacgcg gctctcgcgg catcattgct gcgctggggc ctgatggtaa
     2821 gccctcacga atcgtagtga tctacacgac ggggagtcag gccactatgg acgaacgaaa
     2881 taggcagatc gctgagatcg gtgcctcact gatcaagcac tggtaaccac tgcagtggtt
     2941 tagcatttgc ggccgctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3001 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgacatgt
     3061 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3541 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3601 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttgaagcttg
     3781 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
     3841 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
     3901 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
     3961 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
     4021 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
     4081 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
     4141 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
     4201 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
     4261 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
     4321 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
     4381 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
     4441 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
     4501 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
     4561 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
     4621 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
     4681 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     4741 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     4801 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     4861 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     4921 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
     4981 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
     5041 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
     5101 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
     5161 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
     5221 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
     5281 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
     5341 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
     5401 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
     5461 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     5521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5641 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5701 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     5761 acccagtcag ctccttccgg tgggcgcggg gcatgactaa agaaatagat tttgggccgg
     5821 gcacggtggc tcatgcctgt aatcccagca ctttaggagg ccgaggcgtg tggatcacct
     5881 gaagtcagga gttcaagacc agcctggcca acatggtgag accttgtatc tactaaaaat
     5941 acaaaaacta gccgggcttg gtggcgcatg cctgtagtcc cagctactca ggaggctgag
     6001 gcaggagaat cgcttgaacc caggaggtaa atgttgcagt aagccgagat tgccccactg
     6061 cactccagcc tggatgacaa gagtgagact ccatctcaaa aaaaaaaaaa aaaaaaaaga
     6121 ttttaggggt aaaatatttt gctttccttc agtggctg
//
