LOCUS       sequence-197092-        5986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with OAS3 enhancer inserted downstream 
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase INF enhancer vector_ORI_OAS3
FEATURES             Location/Qualifiers
     source          1..5986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1831..1848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2006..2074,2075..2866)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3056..3644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3136..3155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3743..3752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3749..5401
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    5445..5566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5456..5475)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5510..5529
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agatggaatt tttattggaa ctgcattaca cgtataaatt atttaggaag agttggcttc
       61 tgaagagggc agctctgtgg gcttgggctg ggtttgaatc cagccagaca actttccagc
      121 tgtgttacct tggacagtta cctagttcct ctgtaccttg acttcctcat ctgtcaaatg
      181 ggtgatgata atagcaccta caccatggtc gttgggagga gtcagtgaga gtctccaagt
      241 gtgctttatt atcgttggtg gtggtagtgg tgtttagaac gcatccctca gtcagacagc
      301 gaggtaggac ttctccgttt attaaatcct gacctttttt ttccatgtaa aacctgcacg
      361 tttctgaaat gctcagagta cgttactcag tatgtaccca tacgttctgt gggtatactt
      421 tgttaggttg tgattagttc gttggagctg gtggttgcag ggacggctgg aagcaaggag
      481 atgaaggaga ggaagtcgta gctgggaagg ggaccaggaa gtgggtgtca ggtccaagag
      541 ctgctagaaa gaaacgaaac tgaaagcagg gaatttccca agtttgggga agacaggaac
      601 tgcagcgccc ctccccgttt cacgccacgc gcgggaccga ggacctagga cctggccagc
      661 tgggcgtggt tcggagagcc gggcgggaaa acgaaaccag aaatccgaag gccgcgccag
      721 agccctgctt ccccttgcat ctgcgccggg aggccatgga cttgtacagc accccggccg
      781 ctgcgctgga caggttcgtg gccagaaagc tgcagccgcg gaaggagttc gtagagaagg
      841 cgcggcgcgc tctgggcgcc ctggccgctg ctctgaggga gcgcgggggc cgcctcggtg
      901 ctgctgcccc gcgggtgctg aaaactgtca aggtgaggtc ccacctcggg gtctttatgt
      961 gtccaaaggg caaaggggag tcctgggagg acgcttaagc ctcacatagg cttacggtgg
     1021 gggtggcttt atccgcttgt gcacctcatt catttcttta acaaatactt ccaatgtgcc
     1081 agccccgtgc taactccccc gaacatacga gccaggtagg tactcttaag cccgcttgac
     1141 agatagagaa aatgaggcac agagaggtac agtgacgtgt ccgatcctgt aagaggccca
     1201 gccaggattc aaatccaagt agcctgattc ctgcatctct actctcagag gctgcttctt
     1261 atccattcat tcactcattc actcattctc ccagtcattc aaacagactg tgggcctcac
     1321 actatgccag accttaagga tacagaagta ataacactcc gcgtaacagc cagagtagcc
     1381 caatttattg agacattatc aaataacaat gaaaattatc tcattaaatg agtattacct
     1441 catttcatcc ccacgttcag tgaggcaggt attattgtac ctgttttatg gatagggaaa
     1501 ctgaggctag acgctaaatt actactctga gtcacatctt tttttttttt tttttttttt
     1561 ttgagacagg gtctcgctct gtcacccagg ctggagtgca gtagcatgat cttggctcac
     1621 agcaacctcc gcctcctggg ctcaagttat tctcacacct cagcctccca agtagctggc
     1681 actacaggtg cacactacca tgcctggcta attatttgca ttttttgtag agatagagtt
     1741 ttgccgtgtt gcccagtcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     1801 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1921 aggggataac gcaggaaaga acatgtcttg agagacgtac tagtaaccct gataaatgct
     1981 tcaataatat tgaaaaagga cgagtatgag catccaacat tttcgtgtcg cactcattcc
     2041 cttctttgcg gcattttgct tgcctgtttt tgcacacccc gaaacgctgg tgaaagtaaa
     2101 agatgctgaa gatcaactgg gtgcaagagt gggctatatc gaactggatc tcaatagcgg
     2161 caagatcctt gagtctttcc gccccgaaga acgattcccg atgatgagca ctttcaaagt
     2221 actgctatgt ggcgcggtgt tgtcccgtat agacgccggg caagagcagc ttggtcgccg
     2281 tatacactac tcacaaaacg acttggttga gtactcgccg gtcacggaaa agcatcttac
     2341 ggatggcatg acggtaagag aattgtgtag tgctgccatt accatgagcg acaacaccgc
     2401 ggccaactta cttctgacaa cgatcggagg ccctaaggag ctgactgcat ttcttcataa
     2461 tatgggtgat catgtgaccc ggcttgaccg ctgggaacca gagttgaacg aagccatacc
     2521 gaacgacgag cgtgatacca cgatgccagt agcaatggcc acaactcttc ggaaactact
     2581 cactggcgaa cttcttactc tagcatcacg acagcagctc atagactgga tggaggcgga
     2641 caaagtagca ggaccacttc ttcgctcggc cctccctgct ggctggttca ttgctgacaa
     2701 atcgggggcc ggtgaacgcg gctctcgcgg catcattgct gcgctggggc ctgatggtaa
     2761 gccctcacga atcgtagtga tctacacgac ggggagtcag gccactatgg acgaacgaaa
     2821 taggcagatc gctgagatcg gtgcctcact gatcaagcac tggtaaccac tgcagtggtt
     2881 tagcatttgc ggccgctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2941 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgacatgt
     3001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttgaagcttg
     3721 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
     3781 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
     3841 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
     3901 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
     3961 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
     4021 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
     4081 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
     4141 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
     4201 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
     4261 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
     4321 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
     4381 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
     4441 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
     4501 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
     4561 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
     4621 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     4681 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     4741 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     4801 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     4861 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
     4921 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
     4981 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
     5041 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
     5101 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
     5161 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
     5221 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
     5281 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
     5341 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
     5401 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     5461 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5521 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5581 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5641 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     5701 acccagtcag ctccttccgg tgggcgcggg gcatgactag gccatgaaga tagaagcgca
     5761 atggtcctgg attcaaatct ccactctgca gcttatagct tacagtccgc ttagctttgt
     5821 gcccattcca agaggatttc cctattgtag ctttacaatg ttttaatatg tagtattgtt
     5881 gtgcaaggta ccatcctaaa ctgactgact ttccttcctc cttctcctcc aaaataattt
     5941 ttaattcttg cagacagact ttagatcgtg tgtttgaagt ttttaa
//
