LOCUS       sequence-197105-        8373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To define the role of Mena/VASP in tension-sensitive actin assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman 
            BD, Grashoff C, Schwartz MA, Yap AS
  TITLE     Tension-sensitive actin assembly supports contractility at the 
            epithelial zonula adherens.
  JOURNAL   Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 
            10.1016/j.cub.2014.06.028. Epub 2014 Jul 24.
  PUBMED    25065757
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.06.028"; journalName: "Curr Biol"; date: 
            "2014-08-4- 4"; volume: "24"; issue: "15"; pages: "1689-99"
COMMENT     Sequence Label: MRLC-DD-pIRES-EGFP-AP4-mito
FEATURES             Location/Qualifiers
     source          1..8373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        23..402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        403..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     557..577
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     651..670
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        651..669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1255..1828
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1278..1830
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1422..1439)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1649..1668
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1845..1854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1851..1853,1854..1856,1857..2567)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1851..1853,1854..1856,1857..2567)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1896..1917)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2157..2176)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2504..2525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3752..3769)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3758..3982
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4028..4456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4115..4134)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4325..4346
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4465..4485)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4470..4799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4650..4785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4712..4731
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4866..5660
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4920..4939)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5530..5549
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5834..5955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5871..5890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5925..5944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6004..6020)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6004..6020)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6017..6039)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6028..6044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6052..6058,6059..6076,6077..6082))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6097..6118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6235..6252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6406..6994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6486..6505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7165..7956,7957..8025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7788..7807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8026..8130)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8205..8224)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
       61 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      121 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      181 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      241 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      301 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      361 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      421 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      481 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      541 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      601 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      661 cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagacg ccaccatgtc
      721 cagcaagcgg gccaaagcca agaccaccaa gaagcggcca cagcgggccg acgacaatgt
      781 cttcgcaatg tttgaccagt cccagatcca ggagtttaag gaagctttca acatgattga
      841 ccagaaccgt gatggcttca ttgacaagga ggacctgcac gacatgctgg cctcgctggg
      901 gaagaacccc acagacgaat acctggaggg catgatgagc gaggccccgg ggcccatcaa
      961 cttcaccatg ttcctcacca tgtttgggga gaagctgaac ggcacggacc ccgaggatgt
     1021 gattcgcaac gcctttgcct gcttcgacga ggaagcctca ggtttcatcc atgaggacca
     1081 cctccgggag ctgctcacca ccatgggtga ccgcttcaca gatgaggaag tggacgagat
     1141 gtaccgggag gcacccattg ataagaaagg caacttcaac tacgtggagt tcacccgcat
     1201 cctcaaacat ggcgccaagg ataaagacga ctaactcgag cggccgccaa ttggcccctc
     1261 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     1321 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     1381 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     1441 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     1501 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     1561 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     1621 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     1681 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     1741 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     1801 ggttttcctt tgaaaaacac gatgataata tggccacaga attcgccacc atggtgagca
     1861 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1921 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1981 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2041 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2101 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2161 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2221 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2281 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2341 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     2401 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     2461 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2521 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     2581 ctgtaaatgc ttcggacgcc ccgccaccac ctacggatga agagttagga cttgctttgc
     2641 cagagacacc aatgcttctt ggttttaatg ctcctgctac atcagaaccg agctcattcg
     2701 aagctccacc accacctacg gatgaagagt taagacttgc tttgccagag acgccaatgc
     2761 ttcttggttt taatgctcct gctacatcgg aaccgagctc gttcgaagct ccaccgcctc
     2821 caacagaaga tgaactagaa atcatccggg aaacagcatc ctcgctagat tctagtttta
     2881 caagagggga tttagctagt ttgagaaatg ctattaatcg ccatagtcaa aatttctctg
     2941 atgccccacc aatcccaaca gaagaagagt tgaacgggag aggcggtaga ccaacatctg
     3001 aagaatttag ttcgctgaat agtggtgatt ttacagatga cgaaaacagc gagacaacag
     3061 aagaagaaat tgatcgccta gctgatttaa gagatagagg aacaggaaaa cactcaagaa
     3121 atgcgggttt tttaccatta aatccgtttg ctagcagccc ggttccttcg ttaagtccaa
     3181 aggtatcgaa aataagcgca ccggctctga taagtgacat aactaaaaaa acgccattta
     3241 agaatccatc acagccatta aatgtgttta ataaaaaaac tacaacgaaa acagtgacta
     3301 aaaaaccaac ccctgtaaag accgcaccaa agctagcaga acttcctgcc acaaaaccac
     3361 aagaaaccgt acttagggaa aataaaacac cctttataga aaaacaagca gaaacaaaca
     3421 agcagtcaat taatatgccg agcctaccag taatccaaaa agaagctaca gagagcgata
     3481 aagaggaaat gaaaccacaa accgaggaaa aaatggtaga ggaaagcgaa tcagctaata
     3541 acgcaaacgg aaaaaatcgt tctgctggca ttgaagaagg aaaactaatt gctaaaagtg
     3601 cagaagacga aaaagcgaag gaagaaccag ggaaccatac gacgttaatt cttgcaatgt
     3661 tagctattgg cgtgttctct ttaggggcgt ttatcaaaat tattcaatta agaaaaaata
     3721 attaaaagct taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3781 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3841 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3901 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3961 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4021 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4441 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4501 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4621 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4801 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4861 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4921 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4981 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5041 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5101 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5161 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5221 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5281 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5341 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5401 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5461 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5521 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5581 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5641 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5701 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5761 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5821 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5881 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5941 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6001 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6061 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6841 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7141 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7201 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7261 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7321 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7381 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7441 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7501 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7561 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7621 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7681 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7741 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7801 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7861 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7921 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7981 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8041 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8101 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8161 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8221 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8281 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8341 cttagggtta ggcgttttgc gctgcttcgc gat
//
