LOCUS       sequence-197122-       10138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TREAT-ARE reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger 
            J, Stadler MB, Giorgetti L, Chao JA
  TITLE     The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in
            Single Cells.
  JOURNAL   Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 
            10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
  PUBMED    29056324
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.09.030"; journalName: "Mol Cell"; date: 
            "2017-11-2- 2"; volume: "68"; issue: "3"; pages: "615-625.e9"
COMMENT     Sequence Label: pSF4 TetCMV intron Renilla STOP 12xPP7 
            TNF-alpha-AR...
FEATURES             Location/Qualifiers
     source          1..10138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3716..3763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3809..3827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3845..3863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3881..3899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3917..3935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3953..3971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3989..4007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4025..4043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4088..4112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4189..4321
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4304..4323
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4366..4384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4388..4397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4394..5329
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    7931..8065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8009..8028
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8173..8206)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8259..8275)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(8291..8313)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8724..9081
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9144..9737
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtcatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcc
     2641 aagctaagtg aaagactctg tctcaaaaac caacctgaca cctgagccgg agcgttggct
     2701 ctcactacca tagaatattt tttcacaaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca cacagtcata cacatagaca cagtcacaca cacacacatg cacacacaca
     2821 cacacacact cacacacaca catacataca cacattcaca cacacccata cacacatttt
     2881 cacagtcaca cacacaaaca caatcacaca cacacacatc aatcaatcaa tcaatcaata
     2941 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
     3001 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
     3061 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
     3121 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
     3181 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
     3241 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
     3301 accaaattaa ttttgataca tgctgcatgt gtgaaagacc cccgctgacg ggtagtcaat
     3361 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3421 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3481 tgaggggttg tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct
     3541 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
     3601 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
     3661 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt
     3721 tcctatactt tctagagaat aggaacttcg gaataggaac ttcgcggcag atcttttgcg
     3781 gccgctttgg gccggccgaa ttctttactc cctatcagtg atagagaacg tatgaagagt
     3841 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3901 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3961 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     4021 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     4081 aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt
     4141 cttataccaa ctttccgtac cacttcctac cctcgtaaaa ccggtcaggt aagtatcaag
     4201 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     4261 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     4321 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     4381 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
     4441 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
     4501 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
     4561 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
     4621 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
     4681 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
     4741 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
     4801 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
     4861 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
     4921 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
     4981 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
     5041 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
     5101 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
     5161 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
     5221 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
     5281 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaag tcgacagatc
     5341 ctacctgcaa agcaccagag gatatggcct cggtgcctca gcatcttcag atgtgctgtc
     5401 actgtccaat cttcaagcca gcagagcata tgggctcgct ggctccactg tcattacagt
     5461 ggacctgatc ttctccaatc aaaggagcag aggatatggc ctcgctccct ccgactacat
     5521 caggctggaa gtgacatcca tcattcatcc cagcagagca tatgggctcg ctgggaccac
     5581 agcatgcctc gaagtgctga gagactatca tcaaaccgac agaggatatg gcctcgtcgg
     5641 ctcctactcc ctcctggtgg aatacttctc cactcttcaa cccagcagag catatgggct
     5701 cgctgggtcc atcagagata caagatctct cgacagatcc tacctgcaaa gcaccagagg
     5761 atatggcctc ggtgcctcag catcttcaga tgtgctgtca ctgtccaatc ttcaagccag
     5821 cagagcatat gggctcgctg gctccactgt cattacagtg gacctgatct tctccaatca
     5881 aaggagcaga ggatatggcc tcgctccctc cgactacatc aggctggaag tgacatccat
     5941 cattcatccc agcagagcat atgggctcgc tgggaccaca gcatgcctcg aagtgctgag
     6001 agactatcat caaaccgaca gaggatatgg cctcgtcggc tcctactccc tcctggtgga
     6061 atacttctcc actcttcaac ccagcagagc atatgggctc gctgggtcca tcagagatac
     6121 aagatctctc gagccgcggt gcgtaagtct gttaacagag attatttatt atttatttat
     6181 tatttattta tttactgaca ctgaacgtaa tccagacgcg taagtcaggc cggaaaattc
     6241 ccgccaccgg aagttgagta gacggtgctg cctgcgactc aaccccagga ggactgggtg
     6301 aacaaagctg cgaagtgatc catgtaagcc ctcagaaccg tctcggaaag aggaccccac
     6361 atgttgtagc ttcaaggccc aatgtcagac cacgccatgg cgtgccactc tgcggagagt
     6421 gcagtctgcg acagtgcccc aggaggactg ggtgaggatc ctacctacaa acggcacgag
     6481 catcagccgt gccgcccagc atcttcatca ctgtccaatc ttcaagcgtg cacgatcacg
     6541 gcacgctcca ctgtcattac agtcttatcc aatcaaactc cagcagcatc agcgctggag
     6601 ctccgactac atcaggacat ccatcattca acgctcgacg atcacgcgag cgtccacagc
     6661 atgcctcaga cactatcatc aaacggctgc acgatcacgg cagccgctcc tactgcctct
     6721 acgttatcga ctcttctgcg acgagcatca gccgtcgcaa catcggacat accagatcct
     6781 acctacaaac ggcacgagca tcagccgtgc cgcccagcat cttcatcact gtccaatctt
     6841 caagcgtgca cgatcacggc acgctccact gtcattacag tcttatccaa tcaaactcca
     6901 gcagcatcag cgctggagct ccgactacat caggacatcc atcattcaac gctcgacgat
     6961 cacgcgagcg tccacagcat gcctcagaca ctatcatcaa acggctgcac gatcacggca
     7021 gccgctccta ctgcctctac gttatcgact cttctgcgac gagcatcagc cgtcgcaaca
     7081 tcggacatac cagatcctac ctacaaacgg cacgagcatc agccgtgccg cccagcatct
     7141 tcatcactgt ccaatcttca agcgtgcacg atcacggcac gctccactgt cattacagtc
     7201 ttatccaatc aaactccagc agcatcagcg ctggagctcc gactacatca ggacatccat
     7261 cattcaacgc tcgacgatca cgcgagcgtc cacagcatgc ctcagacact atcatcaaac
     7321 ggctgcacga tcacggcagc cgctcctact gcctctacgt tatcgactct tctgcgacga
     7381 gcatcagccg tcgcaacatc ggacatacca gatcctacct acaaacggca cgagcatcag
     7441 ccgtgccgcc cagcatcttc atcactgtcc aatcttcaag cgtgcacgat cacggcacgc
     7501 tccactgtca ttacagtctt atccaatcaa actccagcag catcagcgct ggagctccga
     7561 ctacatcagg acatccatca ttcaacgctc gacgatcacg cgagcgtcca cagcatgcct
     7621 cagacactat catcaaacgg ctgcacgatc acggcagccg ctcctactgc ctctacgtta
     7681 tcgactcttc tgcgacgagc atcagccgtc gcaacatcgg acataccaga tcttcaatcg
     7741 atttcgcgcg ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
     7801 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
     7861 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
     7921 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     7981 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     8041 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     8101 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     8161 tgattatgat ctgaagttcc tatactattt gaagaatagg aacttcggaa taggaacttc
     8221 ggatctcata tggccatatg ggggcgccta gagaaggagt gagggctgga taaagggagg
     8281 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
     8341 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
     8401 cttcccaggt cacgatgtac cccacctggt ctgggtgtcc atgcgggcca ggtgaaaaga
     8461 ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga
     8521 cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta
     8581 ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg
     8641 gagtggtaac aggctagccc gggcccatat tttcagacaa atacagaaac acagtcagac
     8701 agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa
     8761 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
     8821 ccacatatcc ctcctctaag gggggtgcac caaagagtcc taaacgatcg ggattttagg
     8881 actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc agggttgcgg
     8941 ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
     9001 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
     9061 agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc
     9121 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
     9181 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     9241 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     9301 gttgtgggct cttttattga gctcggggag cataagcgcg cgaacagaag cgagaagcga
     9361 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
     9421 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
     9481 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     9541 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
     9601 ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttt tctattctca
     9661 gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
     9721 aggtggggtc tttcattcac atgtatatgt caaaaataaa aatcaactaa ttgactagta
     9781 attaatatga ctggcataat gggaaattga tcctgacaga tgcaaactgg cttgctcagc
     9841 agcgcattta tgttgtcaac tgaggaagga aacgttaatg acagaaactc taagtaattt
     9901 ccacgtttat ctatttttat ttatactagc tttggtaaca ggaatattgc agcattcatg
     9961 cacattgaaa cccttatgaa ataaaaacat ctgtgcattt aaaatggaat taacatttta
    10021 aatgttaaaa aaagctggct tagcttcccc ccgcccccta gggcatagaa caagtcaaat
    10081 gctttatata tttgagtttg ggatgtatta ggaaactcct aagagcaaag ctagcttg
//
