LOCUS       sequence-19714-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3-ELAM-luc
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(362..380)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 agaatcatgc atttttgtca tattaataaa attgcatata cgatataaag gcatggacaa
       61 aggtgaagta gcttcaagag acagagtttc tgacatcatt gtaattttaa gcatcgtgga
      121 tattcccggg aaagtttttg gatgccattg gggatttcct ctttactgga tgtggacaat
      181 atcctcctat aagcttgggg attacatagt tcaggacttg ggcataaaag gcagagcagg
      241 gcacggatct ggatctaagt aagcttggca ttccggtact gttggtaaag ccaccatgga
      301 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      361 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      421 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      481 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      541 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      601 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      661 gcctaccgtg gtgttcgttt cc
//
