LOCUS       sequence-197146-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged metap2 in mammalian cells to make lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria 
            SM, Harper JW, Gygi SP, Sabatini DM
  TITLE     SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2017 Nov 10;358(6364):813-818. doi: 
            10.1126/science.aao3265.
  PUBMED    29123071
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-10- 10"; volume: "358"; 
            issue: "6364"; pages: "813-818"
COMMENT     Sequence Label: pLJM1-FLAG-metap2
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    850..1083
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1268..1312
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1619..1822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1773..1793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1819..1843
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2053..2071
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2053..2070
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2084..2103
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2162..2185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3698..3815
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3864..4374
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4293..4311
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4396..4995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4396..4415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4892..4912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5123..5356
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5428..5549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5465..5484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5519..5538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5589..5724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5744..5763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5745..5763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5773..5790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5773..5789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5782..5804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5931..6386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6018..6037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6228..6249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6412..6516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6517..6585,6586..7377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6735..6754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7548..8136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8037..8056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8290..8307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8460..8465,8466..8483,8484..8490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8498..8514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8503..8525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8522..8538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8522..8538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8557..8577
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8559..8577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8605..8831
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
      721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
      781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
      841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
      901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
      961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1021 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1081 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1141 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1201 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1261 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1321 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1381 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1441 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggaccccata tgccaagtac
     1501 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1561 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1621 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1681 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1741 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1801 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     1861 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg
     1921 gtgcattgga acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagagtcta
     1981 taggcccacc cccttggctt cgttagaacg cggctacaat taatacataa ccttatgtat
     2041 catacacata cgatttaggt gacactatag aataacatcc actttgcctt tctctccaca
     2101 ggtgtccact cccaggtcca actgcacctc ggttctatcg attgaattca ccatggaaat
     2161 ggactacaag gatgacgatg acaagggtgg tggtggtggt gcgtcgacga tggcgggtgt
     2221 ggaggaggta gcggcctccg ggagccacct gaatggcgac ctggatccag acgacaggga
     2281 agaaggagct gcctctacgg ctgaggaagc agccaagaaa aaaagacgaa agaagaagaa
     2341 gagcaaaggg ccttctgcag gggaacagga acctgataaa gaatcaggag cctcagtgga
     2401 tgaagtagca agacagttgg aaagatcagc attggaagat aaagaaagag atgaagatga
     2461 tgaagatgga gatggcgatg gagatggagc aactggaaag aagaagaaaa agaagaagaa
     2521 gaagagagga ccaaaagttc aaacagaccc tccctcagtt ccaatatgtg acctgtatcc
     2581 taatggtgta tttcccaaag gacaagaatg cgaataccca cccacacaag atgggcgaac
     2641 agctgcttgg agaactacaa gtgaagaaaa gaaagcatta gatcaggcaa gtgaagagat
     2701 ttggaatgat tttcgagaag ctgcagaagc acatcgacaa gttagaaaat acgtaatgag
     2761 ctggatcaag cctgggatga caatgataga aatctgtgaa aagttggaag actgttcacg
     2821 caagttaata aaagagaatg gattaaatgc aggcctggca tttcctactg gatgttctct
     2881 caataattgt gctgcccatt atactcccaa tgccggtgac acaacagtat tacagtatga
     2941 tgacatctgt aaaatagact ttggaacaca tataagtggt aggattattg actgtgcttt
     3001 tactgtcact tttaatccca aatatgatac gttattaaaa gctgtaaaag atgctactaa
     3061 cactggaata aagtgtgctg gaattgatgt tcgtctgtgt gatgttggtg aggccatcca
     3121 agaagttatg gagtcctatg aagttgaaat agatgggaag acatatcaag tgaaaccaat
     3181 ccgtaatcta aatggacatt caattgggca atatagaata catgctggaa aaacagtgcc
     3241 gattgtgaaa ggaggggagg caacaagaat ggaggaagga gaagtatatg caattgaaac
     3301 ctttggtagt acaggaaaag gtgttgttca tgatgatatg gaatgttcac attacatgaa
     3361 aaattttgat gttggacatg tgccaataag gcttccaaga acaaaacact tgttaaatgt
     3421 catcaatgaa aactttggaa cccttgcctt ctgccgcaga tggctggatc gcttgggaga
     3481 aagtaaatac ttgatggctc tgaagaatct gtgtgacttg ggcattgtag atccatatcc
     3541 accattatgt gacattaaag gatcatatac agcgcaattt gaacatacca tcctgttgcg
     3601 tccaacatgt aaagaagttg tcagcagggg agatgactat taagcggccg cagtgagaat
     3661 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3721 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3781 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3841 atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg
     3901 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     3961 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     4021 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     4081 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     4141 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     4201 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     4261 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     4321 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     4381 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4441 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4501 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4921 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4981 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5041 ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5101 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5161 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5341 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5401 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     5461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5521 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     5581 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5641 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5701 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     5761 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5821 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5881 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     5941 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6001 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6061 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6121 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6181 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6241 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6301 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6361 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8221 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8281 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8521 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8581 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8641 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8701 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8761 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     8821 gatacataaa c
//
