LOCUS       sequence-197148-        8708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria 
            SM, Harper JW, Gygi SP, Sabatini DM
  TITLE     SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2017 Nov 10;358(6364):813-818. doi: 
            10.1126/science.aao3265.
  PUBMED    29123071
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-10- 10"; volume: "358"; 
            issue: "6364"; pages: "813-818"
COMMENT     Sequence Label: pLJM1-FLAG-Kaptin
FEATURES             Location/Qualifiers
     source          1..8708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    281..398
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        447..957
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     876..894
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             979..1578
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(979..998)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1475..1495
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1706..1939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2011..2132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2048..2067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2102..2121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2172..2307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2327..2346)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2328..2346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2356..2373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2356..2372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2365..2387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2514..2969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2601..2620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2811..2832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2995..3099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3100..3168,3169..3960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3318..3337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4131..4719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4620..4639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4873..4890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5007..5028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5043..5048,5049..5066,5067..5073)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5081..5097
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5086..5108
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5105..5121
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5105..5121
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5140..5160
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5142..5160
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5188..5414
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5415..5595
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5642..5767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6264..6497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6682..6726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7033..7236
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7187..7207
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7233..7257
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        7467..7485
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7467..7484
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     7498..7517
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7576..7599
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccagtccc ctgctggcca tggctcacgt ggacctgacc ggggatgggc tgcaggagct
       61 tgccgtggtc tccctgaagg gcgtgcacat cctgcagcac agcctgattc aggcctcaga
      121 gctggtcttg acccggcttc gacatcaagt ggagcagagg agacgtcggc tacaggggtt
      181 ggaggacggg gcaggtgcag ggcctgctga gaatgcagcc tcttaagcgg ccgcagtgag
      241 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      301 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      361 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      421 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
      481 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
      541 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
      601 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
      661 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
      721 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
      781 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
      841 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
      901 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
      961 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     1021 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     1081 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     1141 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     1201 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     1261 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     1321 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     1381 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     1441 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     1501 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     1561 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     1621 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     1681 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     1741 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1801 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1861 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1921 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     1981 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     2041 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2101 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     2161 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2221 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2281 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     2341 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2401 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2461 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     2521 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2581 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2641 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2701 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2761 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2821 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2881 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2941 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3001 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3061 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3121 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3181 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3241 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3301 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3361 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3421 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3481 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3541 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3601 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3661 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3721 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3781 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3841 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3901 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3961 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4021 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4081 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4141 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4201 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4261 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4321 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4381 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4441 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4501 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4561 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4621 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4681 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4741 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4801 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4861 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4921 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4981 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     5041 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     5101 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     5161 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     5221 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     5281 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     5341 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     5401 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5461 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5521 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5581 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     5641 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     5701 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     5761 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     5821 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     5881 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     5941 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6001 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6061 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     6121 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6181 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     6241 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     6301 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     6361 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     6421 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     6481 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6541 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6601 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6661 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6721 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     6781 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     6841 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     6901 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6961 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7021 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     7081 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     7141 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7201 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     7261 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     7321 cgcggccggg aacggtgcat tggaacgcgg attccccgtg ccaagagtga cgtaagtacc
     7381 gcctatagag tctataggcc cacccccttg gcttcgttag aacgcggcta caattaatac
     7441 ataaccttat gtatcataca catacgattt aggtgacact atagaataac atccactttg
     7501 cctttctctc cacaggtgtc cactcccagg tccaactgca cctcggttct atcgattgaa
     7561 ttcaccatgg aaatggacta caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg
     7621 acgatgatgg gggaggcggc cgtggccgcg gggccttgtc cgttgcgcga ggacagcttc
     7681 acgcgcttct cgtcgcagag caatgtgtac gggctggcag gcggcgccgg cgggcgcggg
     7741 gagctgctgg ccgccaccct taaaggcaag gtgctcggct tccgctacca agacctccga
     7801 cagaaaatcc ggccagtggc caaggagctg cagttcaact acattcccgt ggatgcggag
     7861 attgtctcca tcgacacttt caacaagtca ccccccaagc ggggtctggt tgtggggatc
     7921 acgttcatca aggattcagg ggacaagggc agccccttcc tgaacattta ctgcgactac
     7981 gagcccggct ctgagtacaa ccttgactct attgcccaga gctgcctgaa cctggagctc
     8041 cagttcactc cgttccagct gtgccatgcg gaggtccagg tcggggatca acttgagact
     8101 gtgtttctct tgagtgggaa cgacccggcc attcatctct acaaggagaa cgaggggctg
     8161 catcagtttg aggaacagcc cgtggaaaac ctcttcccag agctgacgaa cctgaccagt
     8221 agcgtcctct ggctggacgt ccacaacttc cccggcacgt cccggcgcct ctcagctctg
     8281 ggctgtcaga gtggttatgt ccgtgtcgcc cacgtggacc agcggagtcg agaggttctg
     8341 cagatgtggt cggtcctgca ggacggtccc atctcccgag tgattgtgtt cagcctctcg
     8401 gccgccaagg agaccaagga caggccacta caagatgagt acagcgtgct cgtggccagc
     8461 atgttggagc cagcagtggt gtatcgggac ctgctgaacc ggggtcttga agaccagctt
     8521 ctcctgcccg gcagtgacca gtttgacagc gtcctctgca gcctggtcac cgatgtggat
     8581 ttggatgggc ggccagaagt cctggtggcc acctatggac aggaactgct gtgttataag
     8641 taccggggcc cagagtcggg gcttcctgag gcccagcacg ggttccatct gctgtggcag
     8701 cggagctt
//
