LOCUS       sequence-197161-        8538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On Snail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, 
            Iacobuzio-Donahue CA, Massague J
  TITLE     TGF-beta Tumor Suppression through a Lethal EMT.
  JOURNAL   Cell. 2016 Feb 25;164(5):1015-30. doi: 10.1016/j.cell.2016.01.009. 
            Epub 2016 Feb 18.
  PUBMED    26898331
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.009"; journalName: "Cell"; date: 
            "2016-02-25- 25"; volume: "164"; issue: "5"; pages: "1015-30"
COMMENT     Sequence Label: pLVX Tight Puro Snail
FEATURES             Location/Qualifiers
     source          1..8538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..321
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    158..176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    193..211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    229..247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     294..318
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             344..367
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1172..1671
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1207..1230)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1604..1623
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1692..2291
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1692..1711)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2188..2208
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2308..2896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2361..2381)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2779..2790)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3103..3736
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3864..3880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3864..3880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3877..3899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3888..3904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3912..3918,3919..3936,3937..3942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3957..3978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4095..4112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4266..4854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4346..4365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5025..5816,5817..5885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5648..5667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5886..5990)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6038..6172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6075..6094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6129..6148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6340..6973
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7020..7145
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7642..7875
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8060..8104
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8367..8482
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tcactcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
       61 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      121 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
      181 atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      241 atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
      301 ttagtgaacc gtcagatcgc ctggagaagg atccgccacc atggattaca aggacgacga
      361 tgacaaatgc cgcgctcctt cctggtcagg aagccgtccg acccccgccg gaagcccaac
      421 tatagcgagc tgcaggacgc gtgtgtggag ttcaccttcc agcagcccta cgaccaggcc
      481 cacctgctgg ccgccatccc tccgcccgag gtcctcaacc ccgccgcttc gctgcccacc
      541 ctcatctggg actctctcct ggtaccccaa gtgcggccgg ttgcctgggc caccctcccg
      601 ctgcgggaga gccccaaggc cgtagagctg acctcgctgt ccgatgagga cagtggcaaa
      661 agctcccagc cgcccagccc gccctcgccg gcgccgtcgt ccttctcgtc cacctcggcc
      721 tcgtccctgg aggccgaggc cttcatcgcc ttccctggct tgggccaact tcccaagcag
      781 ctggccaggc tctcggtggc caaggacccc cagtcgcgga agatcttcaa ctgcaaatat
      841 tgtaacaagg agtacctcag cctgggcgct ctgaagatgc acatccgaag ccacacgctg
      901 ccttgtgtct gcacgacctg tggaaaggcc ttctctaggc cctggctgct tcagggccac
      961 gtccgcaccc acactggtga gaagccattc tcctgctccc actgcaaccg tgcttttgct
     1021 gaccgctcca acctgcgtgc ccacctccaa acccactcgg atgtgaagag ataccagtgc
     1081 caggcctgtg cccgaacctt ctcccgcatg tccttgctcc acaagcacca agagtctggc
     1141 tgctccggag gccctcgctg agaattctac cgggtagggg aggcgctttt cccaaggcag
     1201 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     1261 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     1321 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     1381 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     1441 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     1501 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     1561 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     1621 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     1681 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     1741 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     1801 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     1861 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     1921 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     1981 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2041 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     2101 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     2161 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     2221 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     2281 ccggtgcctg acgggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     2341 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2401 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2461 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2521 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2581 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2641 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2701 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2761 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2821 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2881 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     2941 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     3001 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     3061 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     3121 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     3181 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     3241 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     3301 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     3361 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     3421 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     3481 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     3541 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3721 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     3781 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     3841 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6001 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     6061 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6121 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     6181 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     6241 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     6301 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     6361 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6421 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     6481 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     6541 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     6601 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     6661 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     6721 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     6781 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6961 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7021 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7081 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     7141 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7201 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7261 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7321 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7381 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7441 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     7501 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7561 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7621 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7681 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7741 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7801 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7861 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7921 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     8281 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     8341 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     8401 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     8461 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     8521 gatgaggccc tttcgtct
//
