LOCUS       sequence-197162-        5575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV expression of CaMPARI, a photoconvertible fluorescent 
            protein-based calcium integrator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, 
            Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER
  TITLE     Neural circuits. Labeling of active neural circuits in vivo with 
            designed calcium integrators.
  JOURNAL   Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922.
  PUBMED    25678659
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-02-13- 13"; volume: "347"; 
            issue: "6223"; pages: "755-60"
COMMENT     Sequence Label: pAAV.hSyn.CaMPARI.WPRE.SV40
FEATURES             Location/Qualifiers
     source          1..5575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             340..357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             364..804
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             1525..1581
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     misc_feature    1597..2185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1650..1670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2068..2079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2188..2204)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2189..2205)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2234..2355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2245..2264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2299..2318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2517..2646
                     /label=AAV2 ITR
     rep_origin      complement(2883..3471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2963..2982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3642..4433,4434..4502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4265..4284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4503..4607)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4633..5088)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4770..4791)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4982..5001
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5160..5289
                     /label=AAV2 ITR
     promoter        join(5371..5575,1..243)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
       61 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
      121 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
      181 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
      241 cagtcgaatt caagctgcta gcaaggatcc gccaccatgc tgcagaacga gcttgctctt
      301 aagttggctg gacttgatat taacaagact ggaggttctc atcatcacca ccaccatgga
      361 tccgaccaac tgactgaaga gcagatcgca gaatttaaag aggctttctc cctatttgac
      421 aaggacgggg atgggacaat aacaaccaag gagctgggga cggtgatgcg gtctctgggg
      481 cagaacccca cagaagcaga gctgcaggac atgatcaatg aagtagatgc cgacggtgac
      541 ggcacaatcg acttccctga gttcctgaca atgatggcaa gaaaaatgaa agacacagac
      601 agtgaagaag aaattagaga agcgttccgt gtgtttgata aggatggcaa tggctacatc
      661 agtgcagcag agcttcgcca cgtgatgaca aaccttggag agaagttaac agatgaagag
      721 gttgatgaaa tgatcaggga agcagacatc gatggggatg gtcaggtaaa ctacgaagag
      781 tttgtacaaa tgatgacagc gaagctcgag tgcgagaaaa tctatgtgcg tgatggagtg
      841 ctgacgggtg atattcatat gatcttgttg cttgaaggaa atgcccatta ccgatgtgac
      901 ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcgt gcactttgtg
      961 gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag
     1021 tatgctgttg ctcattctgg attgcctgac aatgccagac gaggcggtac cggcggatcc
     1081 atggtgagtg cgattaagcc agacatgaag atcaaactcc gtatggaagg caacgtaaac
     1141 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc
     1201 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
     1261 gcattccatt acggcaacag ggtattcgtg aaatatccag acaacataca agactatttt
     1321 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagca tgactttcga agacgggggc
     1381 atttgctatg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
     1441 ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
     1501 atgccgagct ggacgcgttc atcacgtcgt aagtggaata agacaggtca cgcagtcaga
     1561 gctataggtc ggctgagctc ataaaagctt atcgataatc aacctctgga ttacaaaatt
     1621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     1921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     1981 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2161 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt
     2221 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2281 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2341 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2401 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     2461 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
     2521 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     2581 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     2641 gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3781 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4621 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4681 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4741 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4801 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4861 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4921 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4981 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5041 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     5101 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     5161 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg
     5221 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
     5281 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta
     5341 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag
     5401 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc
     5461 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg
     5521 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggc
//
